Journal: Hepatology Communications

Article Title: Ceramide kinase suppresses ferroptosis and protects against alcohol-associated liver disease through the p38 MAPK–HSPB1 pathway

doi: 10.1097/HC9.0000000000000731

Figure Lengend Snippet: Cerk deficiency exacerbates ALD in vivo, whereas overexpression mitigates it. (A) CERK expression in the liver of Cerk-KO mice was assessed by qRT-PCR and Western blotting. (B, C) Representative images of liver specimens and HE staining (scale bar: 50 µm) from control and Cerk-KO mice on Gao-binge ethanol and pair-fed diets. (D, F) Representative images of liver Oil Red O staining (scale bar: 100 µm) and F4/80 (IHC) (scale bar: 50 µm) from Cerk-KO mice following the Gao-binge protocol; n=6/group. (E, G) Liver triglyceride content and serum ALT/AST levels in Cerk-KO mice following the Gao-binge protocol; n=6/group. (H) CERK expression in the liver of Cerk-OE mice was assessed by qRT-PCR and Western blotting. (I, J) Representative images of liver specimens and HE staining (scale bar: 50 µm) from control and Cerk-OE mice on Gao-binge ethanol and pair-fed diets. (K, M) Representative images of liver Oil Red O staining (scale bar: 100 µm) and F4/80 (IHC) (scale bar: 50 µm) from Cerk-OE mice following the Gao-binge protocol; n=6/group. (L, N) Liver triglyceride content and serum ALT/AST levels in Cerk-OE mice following the Gao-binge protocol; n=6/group. ** p <0.01, *** p <0.001, and n.s., no significance. Abbreviations: ALD, alcohol-associated liver disease; CERK, ceramide kinase; Cerk-KO , Cerk knockout; Cerk-OE , Cerk overexpression; CON, Control; EtOH, ethanol; HE, hematoxylin and eosin; IHC, immunohistochemistry; qRT-PCR, reverse transcription quantitative polymerase chain reaction; TG, triglyceride; WT, wild type.

Article Snippet: Cerk knockout ( Cerk-KO ) (C57BL/6JCya- Cerk em1 /Cya) mice were purchased from Cyagen Bio (Strain S-KO-05738).

Techniques: In Vivo, Over Expression, Expressing, Quantitative RT-PCR, Western Blot, Staining, Control, Knock-Out, Immunohistochemistry, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Hepatology Communications

Article Title: Ceramide kinase suppresses ferroptosis and protects against alcohol-associated liver disease through the p38 MAPK–HSPB1 pathway

doi: 10.1097/HC9.0000000000000731

Figure Lengend Snippet: Cerk deficiency intensifies ferroptosis in ALD-affected liver. (A) GSEA for transcripts from control and Cerk-KO mice after ethanol (EtOH) feeding; n=3/group. (B) Genes enriched in cell death, inflammation, and lipid metabolism pathways in Cerk-KO mice after EtOH feeding; n=3/group. (C) Enrichment score plot of ferroptosis in control and Cerk-KO mice after EtOH feeding. (D) Correlation analysis between Cerk and ferroptosis-related genes. (E, H) Representative images of liver iron staining (scale bar: 50 µm) from Cerk-KO and Cerk-OE mice after EtOH feeding. (F, G, I, J) Liver malondialdehyde content and GSH/GSSG ratio in Cerk-KO and Cerk-OE mice after EtOH feeding; n=6/group. ** p <0.01, *** p <0.001, n.s., no significance. Abbreviations: ALD, alcohol-associated liver disease; Cerk-KO , Cerk knockout; EtOH, ethanol; GSEA, gene set enrichment analysis; GSH, reduced glutathione; GSSG, oxidized glutathione; MDA, malondialdehyde.

Article Snippet: Cerk knockout ( Cerk-KO ) (C57BL/6JCya- Cerk em1 /Cya) mice were purchased from Cyagen Bio (Strain S-KO-05738).

Techniques: Control, Staining, Knock-Out