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custom-made multiplex solid phase microarray platform  (Thermo Fisher)


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    Thermo Fisher custom-made multiplex solid phase microarray platform
    Custom Made Multiplex Solid Phase Microarray Platform, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom-made+multiplex+microarray/custom+made+multiplex+solid+phase+microarray+platform/pm40116570-69-14-19
    Average 90 stars, based on 1 article reviews
    custom-made multiplex solid phase microarray platform - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Identification of loci genetically associated with antibodies to specific sets of citrullinated peptides in rheumatoid arthritis patients
    Article Snippet: We combined genotyping data from Illumina Immunochip with serological data for 16 ACPA specificities from a custom-made multiplex microarray (Thermo Fisher Scientific, ImmunoDiagnostic division).

    Article Title: Antibodies to a Citrullinated Porphyromonas gingivalis Epitope Are Increased in Early Rheumatoid Arthritis, and Can Be Produced by Gingival Tissue B Cells: Implications for a Bacterial Origin in RA Etiology
    Article Snippet: Sera from 2,859 RA patients and 372 controls were screened on a custom-made multiplex microarray (ThermoFisher Scientific, ImmunoDiagnostics Division, Uppsala, Sweden) for IgG reactivity against citrullinated peptides derived from Pg PAD (CPP3) and human proteins , as previously described ( , ).

    Microarray:

    Article Title: Identification of loci genetically associated with antibodies to specific sets of citrullinated peptides in rheumatoid arthritis patients
    Article Snippet: We combined genotyping data from Illumina Immunochip with serological data for 16 ACPA specificities from a custom-made multiplex microarray (Thermo Fisher Scientific, ImmunoDiagnostic division).

    Article Title: Antibodies to a Citrullinated Porphyromonas gingivalis Epitope Are Increased in Early Rheumatoid Arthritis, and Can Be Produced by Gingival Tissue B Cells: Implications for a Bacterial Origin in RA Etiology
    Article Snippet: Sera from 2,859 RA patients and 372 controls were screened on a custom-made multiplex microarray (ThermoFisher Scientific, ImmunoDiagnostics Division, Uppsala, Sweden) for IgG reactivity against citrullinated peptides derived from Pg PAD (CPP3) and human proteins , as previously described ( , ).

    Derivative Assay:

    Article Title: Identification of loci genetically associated with antibodies to specific sets of citrullinated peptides in rheumatoid arthritis patients
    Article Snippet: We combined genotyping data from Illumina Immunochip with serological data for 16 ACPA specificities from a custom-made multiplex microarray (Thermo Fisher Scientific, ImmunoDiagnostic division).

    Article Title: Antibodies to a Citrullinated Porphyromonas gingivalis Epitope Are Increased in Early Rheumatoid Arthritis, and Can Be Produced by Gingival Tissue B Cells: Implications for a Bacterial Origin in RA Etiology
    Article Snippet: Sera from 2,859 RA patients and 372 controls were screened on a custom-made multiplex microarray (ThermoFisher Scientific, ImmunoDiagnostics Division, Uppsala, Sweden) for IgG reactivity against citrullinated peptides derived from Pg PAD (CPP3) and human proteins , as previously described ( , ).

    Expressing:

    Article Title: Identification of loci genetically associated with antibodies to specific sets of citrullinated peptides in rheumatoid arthritis patients
    Article Snippet: We combined genotyping data from Illumina Immunochip with serological data for 16 ACPA specificities from a custom-made multiplex microarray (Thermo Fisher Scientific, ImmunoDiagnostic division).

    Article Title: Antibodies to a Citrullinated Porphyromonas gingivalis Epitope Are Increased in Early Rheumatoid Arthritis, and Can Be Produced by Gingival Tissue B Cells: Implications for a Bacterial Origin in RA Etiology
    Article Snippet: Sera from 2,859 RA patients and 372 controls were screened on a custom-made multiplex microarray (ThermoFisher Scientific, ImmunoDiagnostics Division, Uppsala, Sweden) for IgG reactivity against citrullinated peptides derived from Pg PAD (CPP3) and human proteins , as previously described ( , ).

    Affinity Purification:

    Article Title: Identification of loci genetically associated with antibodies to specific sets of citrullinated peptides in rheumatoid arthritis patients
    Article Snippet: We combined genotyping data from Illumina Immunochip with serological data for 16 ACPA specificities from a custom-made multiplex microarray (Thermo Fisher Scientific, ImmunoDiagnostic division).

    Article Title: Antibodies to a Citrullinated Porphyromonas gingivalis Epitope Are Increased in Early Rheumatoid Arthritis, and Can Be Produced by Gingival Tissue B Cells: Implications for a Bacterial Origin in RA Etiology
    Article Snippet: Sera from 2,859 RA patients and 372 controls were screened on a custom-made multiplex microarray (ThermoFisher Scientific, ImmunoDiagnostics Division, Uppsala, Sweden) for IgG reactivity against citrullinated peptides derived from Pg PAD (CPP3) and human proteins , as previously described ( , ).

    Generated:

    Article Title: Identification of loci genetically associated with antibodies to specific sets of citrullinated peptides in rheumatoid arthritis patients
    Article Snippet: We combined genotyping data from Illumina Immunochip with serological data for 16 ACPA specificities from a custom-made multiplex microarray (Thermo Fisher Scientific, ImmunoDiagnostic division).

    Article Title: Antibodies to a Citrullinated Porphyromonas gingivalis Epitope Are Increased in Early Rheumatoid Arthritis, and Can Be Produced by Gingival Tissue B Cells: Implications for a Bacterial Origin in RA Etiology
    Article Snippet: Sera from 2,859 RA patients and 372 controls were screened on a custom-made multiplex microarray (ThermoFisher Scientific, ImmunoDiagnostics Division, Uppsala, Sweden) for IgG reactivity against citrullinated peptides derived from Pg PAD (CPP3) and human proteins , as previously described ( , ).



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    custom-made multiplex microarray  (Thermo Fisher)
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    Thermo Fisher custom-made multiplex microarray
    The anti-CPP3 antibody response in relation to the autoimmune ACPA response. (A) Anti-CPP3 (top panel) and anti-RPP3 (bottom panel) IgG levels in EIRA RA patients (n=2,859) and controls (n=372), measured by multiplex <t>microarray;</t> 98 th percentile cut-off (red line), and 80 th percentile cut-off (blue line) are shown; p-values indicate differences in CPP3/RPP3 IgG levels between groups. (B) Pie chart illustrating the frequency (%) of CPP3+ RA patients (98 th percentile cut-off) in relation to CCP2 status (n=2,859) (top panel), and co-occurrence of anti-CPP3 IgG (98 th percentile cut-off) in relation to eight autoimmune ACPA fine-specificities (measured by multiplex microarray) in EIRA RA patients (n=2,859) (bottom panel); correlation between antibody expression vectors (corrplot package v. 0.77) was used to illustrate co-occurrence, colours signify degree of correlation according to Pearson correlation coefficient (full scale shown on the right). (C) CPP3/RPP3 reactivity in a pool of affinity-purified anti-CCP2 IgG (top panel) and corresponding anti-CCP2 IgG-depleted IgG pool (bottom panel), at different IgG concentrations. AU, arbitrary units; CPP3, citrullinated P. PAD peptide; RPP3, arginine-containing version of CPP3; RA, rheumatoid arthritis; CCP2, cyclic citrullinated peptide(s); OD, optical density; cfc1-cyc, cyclic citrullinated filaggrin peptide 1; Cit-Vim, citrullinated vimentin; Cit-Fib, citrullinated fibrinogen; CEP-1, citrullinated α-enolase peptide 1; Cit-C1, citrullinated triple helical C1 epitope on collagen type II.
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    Image Search Results


    The anti-CPP3 antibody response in relation to the autoimmune ACPA response. (A) Anti-CPP3 (top panel) and anti-RPP3 (bottom panel) IgG levels in EIRA RA patients (n=2,859) and controls (n=372), measured by multiplex microarray; 98 th percentile cut-off (red line), and 80 th percentile cut-off (blue line) are shown; p-values indicate differences in CPP3/RPP3 IgG levels between groups. (B) Pie chart illustrating the frequency (%) of CPP3+ RA patients (98 th percentile cut-off) in relation to CCP2 status (n=2,859) (top panel), and co-occurrence of anti-CPP3 IgG (98 th percentile cut-off) in relation to eight autoimmune ACPA fine-specificities (measured by multiplex microarray) in EIRA RA patients (n=2,859) (bottom panel); correlation between antibody expression vectors (corrplot package v. 0.77) was used to illustrate co-occurrence, colours signify degree of correlation according to Pearson correlation coefficient (full scale shown on the right). (C) CPP3/RPP3 reactivity in a pool of affinity-purified anti-CCP2 IgG (top panel) and corresponding anti-CCP2 IgG-depleted IgG pool (bottom panel), at different IgG concentrations. AU, arbitrary units; CPP3, citrullinated P. PAD peptide; RPP3, arginine-containing version of CPP3; RA, rheumatoid arthritis; CCP2, cyclic citrullinated peptide(s); OD, optical density; cfc1-cyc, cyclic citrullinated filaggrin peptide 1; Cit-Vim, citrullinated vimentin; Cit-Fib, citrullinated fibrinogen; CEP-1, citrullinated α-enolase peptide 1; Cit-C1, citrullinated triple helical C1 epitope on collagen type II.

    Journal: Frontiers in Immunology

    Article Title: Antibodies to a Citrullinated Porphyromonas gingivalis Epitope Are Increased in Early Rheumatoid Arthritis, and Can Be Produced by Gingival Tissue B Cells: Implications for a Bacterial Origin in RA Etiology

    doi: 10.3389/fimmu.2022.804822

    Figure Lengend Snippet: The anti-CPP3 antibody response in relation to the autoimmune ACPA response. (A) Anti-CPP3 (top panel) and anti-RPP3 (bottom panel) IgG levels in EIRA RA patients (n=2,859) and controls (n=372), measured by multiplex microarray; 98 th percentile cut-off (red line), and 80 th percentile cut-off (blue line) are shown; p-values indicate differences in CPP3/RPP3 IgG levels between groups. (B) Pie chart illustrating the frequency (%) of CPP3+ RA patients (98 th percentile cut-off) in relation to CCP2 status (n=2,859) (top panel), and co-occurrence of anti-CPP3 IgG (98 th percentile cut-off) in relation to eight autoimmune ACPA fine-specificities (measured by multiplex microarray) in EIRA RA patients (n=2,859) (bottom panel); correlation between antibody expression vectors (corrplot package v. 0.77) was used to illustrate co-occurrence, colours signify degree of correlation according to Pearson correlation coefficient (full scale shown on the right). (C) CPP3/RPP3 reactivity in a pool of affinity-purified anti-CCP2 IgG (top panel) and corresponding anti-CCP2 IgG-depleted IgG pool (bottom panel), at different IgG concentrations. AU, arbitrary units; CPP3, citrullinated P. PAD peptide; RPP3, arginine-containing version of CPP3; RA, rheumatoid arthritis; CCP2, cyclic citrullinated peptide(s); OD, optical density; cfc1-cyc, cyclic citrullinated filaggrin peptide 1; Cit-Vim, citrullinated vimentin; Cit-Fib, citrullinated fibrinogen; CEP-1, citrullinated α-enolase peptide 1; Cit-C1, citrullinated triple helical C1 epitope on collagen type II.

    Article Snippet: Sera from 2,859 RA patients and 372 controls were screened on a custom-made multiplex microarray (ThermoFisher Scientific, ImmunoDiagnostics Division, Uppsala, Sweden) for IgG reactivity against citrullinated peptides derived from Pg PAD (CPP3) and human proteins , as previously described ( , ).

    Techniques: Multiplex Assay, Microarray, Expressing, Affinity Purification