Review



ctcf  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Proteintech ctcf
    MEG3 interacts with <t>CTCF</t> to restrain the transcriptional activation on CXCR4. (A) Venn diagram illustrates the proteins detected in our MEG3 mass spectrometry data, predicted MEG3 binding proteins from the AnnoLnc2 database, CXCR4-ChIP-seq data from the Cistrome toolkit database, and data from genetic perturbation similarity analysis for CXCR4 upstream regulators from the GPSAdb (Duplicated gene names were removed). (B) RT-PCR was performed to analyze MEG3 lncRNA precipitated from RIP experiment with a CTCF antibody, IgG antibody was used as a control. (C) RNA-FISH was used to demonstrate the co-localization of the full-length MEG3 transcript and the CTCF-EGFP protein. (D) Western blot analysis was conducted on MDA-MB-231 cells following the knockdown of CTCF using two siRNAs to assess the impact on CXCR4 expression (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001). (E) Wound healing assay to evaluate the migratory capacity of MDA-MB-231 cells after the downregulation of CXCR4 caused by CTCF knockdown (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001) (the scale bar represents 500 μm). (F) Transwell assay for MDA-MB-231 after CTCF depletion (n = 3, ∗∗∗p < 0.001) (the scale bar represents 200 μm). (G&H) Wound healing assay and Transwell assay for MEG3 overexpression and CTCF depletion dual treatment (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significance) (the scale bar represents 500 μm for Wound healing assay and 200 μm for Transwell assay). (I) Western blot analysis showing the effect of MEG3 overexpression in combination with CTCF depletion on CXCR4 gene expression (n = 3, ∗∗∗p < 0.001, ns: no significance). (J) Western blot analysis showing the effect of dual overexpression of MEG3 and CTCF on CXCR4 gene expression (n = 3, ∗p < 0.05, ∗∗p < 0.01, ns: no significance).
    Ctcf, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ctcf/product/Proteintech
    Average 93 stars, based on 14 article reviews
    ctcf - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "LncRNA MEG3/CTCF-CXCR4 axis functions in the regulation of breast cancer cell migration"

    Article Title: LncRNA MEG3/CTCF-CXCR4 axis functions in the regulation of breast cancer cell migration

    Journal: Non-coding RNA Research

    doi: 10.1016/j.ncrna.2025.05.014

    MEG3 interacts with CTCF to restrain the transcriptional activation on CXCR4. (A) Venn diagram illustrates the proteins detected in our MEG3 mass spectrometry data, predicted MEG3 binding proteins from the AnnoLnc2 database, CXCR4-ChIP-seq data from the Cistrome toolkit database, and data from genetic perturbation similarity analysis for CXCR4 upstream regulators from the GPSAdb (Duplicated gene names were removed). (B) RT-PCR was performed to analyze MEG3 lncRNA precipitated from RIP experiment with a CTCF antibody, IgG antibody was used as a control. (C) RNA-FISH was used to demonstrate the co-localization of the full-length MEG3 transcript and the CTCF-EGFP protein. (D) Western blot analysis was conducted on MDA-MB-231 cells following the knockdown of CTCF using two siRNAs to assess the impact on CXCR4 expression (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001). (E) Wound healing assay to evaluate the migratory capacity of MDA-MB-231 cells after the downregulation of CXCR4 caused by CTCF knockdown (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001) (the scale bar represents 500 μm). (F) Transwell assay for MDA-MB-231 after CTCF depletion (n = 3, ∗∗∗p < 0.001) (the scale bar represents 200 μm). (G&H) Wound healing assay and Transwell assay for MEG3 overexpression and CTCF depletion dual treatment (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significance) (the scale bar represents 500 μm for Wound healing assay and 200 μm for Transwell assay). (I) Western blot analysis showing the effect of MEG3 overexpression in combination with CTCF depletion on CXCR4 gene expression (n = 3, ∗∗∗p < 0.001, ns: no significance). (J) Western blot analysis showing the effect of dual overexpression of MEG3 and CTCF on CXCR4 gene expression (n = 3, ∗p < 0.05, ∗∗p < 0.01, ns: no significance).
    Figure Legend Snippet: MEG3 interacts with CTCF to restrain the transcriptional activation on CXCR4. (A) Venn diagram illustrates the proteins detected in our MEG3 mass spectrometry data, predicted MEG3 binding proteins from the AnnoLnc2 database, CXCR4-ChIP-seq data from the Cistrome toolkit database, and data from genetic perturbation similarity analysis for CXCR4 upstream regulators from the GPSAdb (Duplicated gene names were removed). (B) RT-PCR was performed to analyze MEG3 lncRNA precipitated from RIP experiment with a CTCF antibody, IgG antibody was used as a control. (C) RNA-FISH was used to demonstrate the co-localization of the full-length MEG3 transcript and the CTCF-EGFP protein. (D) Western blot analysis was conducted on MDA-MB-231 cells following the knockdown of CTCF using two siRNAs to assess the impact on CXCR4 expression (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001). (E) Wound healing assay to evaluate the migratory capacity of MDA-MB-231 cells after the downregulation of CXCR4 caused by CTCF knockdown (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001) (the scale bar represents 500 μm). (F) Transwell assay for MDA-MB-231 after CTCF depletion (n = 3, ∗∗∗p < 0.001) (the scale bar represents 200 μm). (G&H) Wound healing assay and Transwell assay for MEG3 overexpression and CTCF depletion dual treatment (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significance) (the scale bar represents 500 μm for Wound healing assay and 200 μm for Transwell assay). (I) Western blot analysis showing the effect of MEG3 overexpression in combination with CTCF depletion on CXCR4 gene expression (n = 3, ∗∗∗p < 0.001, ns: no significance). (J) Western blot analysis showing the effect of dual overexpression of MEG3 and CTCF on CXCR4 gene expression (n = 3, ∗p < 0.05, ∗∗p < 0.01, ns: no significance).

    Techniques Used: Activation Assay, Mass Spectrometry, Binding Assay, ChIP-sequencing, Reverse Transcription Polymerase Chain Reaction, Control, Western Blot, Knockdown, Expressing, Wound Healing Assay, Transwell Assay, Over Expression, Gene Expression



    Similar Products

    ctcf aid egfp e14tga2  (ATCC)
    96
    ATCC ctcf aid egfp e14tga2
    Ctcf Aid Egfp E14tga2, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ctcf aid egfp e14tga2/product/ATCC
    Average 96 stars, based on 1 article reviews
    ctcf aid egfp e14tga2 - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    ctcf  (EpiCypher)
    93
    EpiCypher ctcf
    Ctcf, supplied by EpiCypher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ctcf/product/EpiCypher
    Average 93 stars, based on 1 article reviews
    ctcf - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    hct116 atcc ccl 247 k562 ctcf maid mclover hu  (ATCC)
    99
    ATCC hct116 atcc ccl 247 k562 ctcf maid mclover hu
    Hct116 Atcc Ccl 247 K562 Ctcf Maid Mclover Hu, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hct116 atcc ccl 247 k562 ctcf maid mclover hu/product/ATCC
    Average 99 stars, based on 1 article reviews
    hct116 atcc ccl 247 k562 ctcf maid mclover hu - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    ctcf chiped dna  (Thermo Fisher)
    99
    Thermo Fisher ctcf chiped dna
    Ctcf Chiped Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ctcf chiped dna/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    ctcf chiped dna - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    ctcf  (Proteintech)
    93
    Proteintech ctcf
    MEG3 interacts with <t>CTCF</t> to restrain the transcriptional activation on CXCR4. (A) Venn diagram illustrates the proteins detected in our MEG3 mass spectrometry data, predicted MEG3 binding proteins from the AnnoLnc2 database, CXCR4-ChIP-seq data from the Cistrome toolkit database, and data from genetic perturbation similarity analysis for CXCR4 upstream regulators from the GPSAdb (Duplicated gene names were removed). (B) RT-PCR was performed to analyze MEG3 lncRNA precipitated from RIP experiment with a CTCF antibody, IgG antibody was used as a control. (C) RNA-FISH was used to demonstrate the co-localization of the full-length MEG3 transcript and the CTCF-EGFP protein. (D) Western blot analysis was conducted on MDA-MB-231 cells following the knockdown of CTCF using two siRNAs to assess the impact on CXCR4 expression (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001). (E) Wound healing assay to evaluate the migratory capacity of MDA-MB-231 cells after the downregulation of CXCR4 caused by CTCF knockdown (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001) (the scale bar represents 500 μm). (F) Transwell assay for MDA-MB-231 after CTCF depletion (n = 3, ∗∗∗p < 0.001) (the scale bar represents 200 μm). (G&H) Wound healing assay and Transwell assay for MEG3 overexpression and CTCF depletion dual treatment (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significance) (the scale bar represents 500 μm for Wound healing assay and 200 μm for Transwell assay). (I) Western blot analysis showing the effect of MEG3 overexpression in combination with CTCF depletion on CXCR4 gene expression (n = 3, ∗∗∗p < 0.001, ns: no significance). (J) Western blot analysis showing the effect of dual overexpression of MEG3 and CTCF on CXCR4 gene expression (n = 3, ∗p < 0.05, ∗∗p < 0.01, ns: no significance).
    Ctcf, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ctcf/product/Proteintech
    Average 93 stars, based on 1 article reviews
    ctcf - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    ctgf  (Proteintech)
    93
    Proteintech ctgf
    MEG3 interacts with <t>CTCF</t> to restrain the transcriptional activation on CXCR4. (A) Venn diagram illustrates the proteins detected in our MEG3 mass spectrometry data, predicted MEG3 binding proteins from the AnnoLnc2 database, CXCR4-ChIP-seq data from the Cistrome toolkit database, and data from genetic perturbation similarity analysis for CXCR4 upstream regulators from the GPSAdb (Duplicated gene names were removed). (B) RT-PCR was performed to analyze MEG3 lncRNA precipitated from RIP experiment with a CTCF antibody, IgG antibody was used as a control. (C) RNA-FISH was used to demonstrate the co-localization of the full-length MEG3 transcript and the CTCF-EGFP protein. (D) Western blot analysis was conducted on MDA-MB-231 cells following the knockdown of CTCF using two siRNAs to assess the impact on CXCR4 expression (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001). (E) Wound healing assay to evaluate the migratory capacity of MDA-MB-231 cells after the downregulation of CXCR4 caused by CTCF knockdown (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001) (the scale bar represents 500 μm). (F) Transwell assay for MDA-MB-231 after CTCF depletion (n = 3, ∗∗∗p < 0.001) (the scale bar represents 200 μm). (G&H) Wound healing assay and Transwell assay for MEG3 overexpression and CTCF depletion dual treatment (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significance) (the scale bar represents 500 μm for Wound healing assay and 200 μm for Transwell assay). (I) Western blot analysis showing the effect of MEG3 overexpression in combination with CTCF depletion on CXCR4 gene expression (n = 3, ∗∗∗p < 0.001, ns: no significance). (J) Western blot analysis showing the effect of dual overexpression of MEG3 and CTCF on CXCR4 gene expression (n = 3, ∗p < 0.05, ∗∗p < 0.01, ns: no significance).
    Ctgf, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ctgf/product/Proteintech
    Average 93 stars, based on 1 article reviews
    ctgf - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    primary antibodies  (EpiCypher)
    93
    EpiCypher primary antibodies
    MEG3 interacts with <t>CTCF</t> to restrain the transcriptional activation on CXCR4. (A) Venn diagram illustrates the proteins detected in our MEG3 mass spectrometry data, predicted MEG3 binding proteins from the AnnoLnc2 database, CXCR4-ChIP-seq data from the Cistrome toolkit database, and data from genetic perturbation similarity analysis for CXCR4 upstream regulators from the GPSAdb (Duplicated gene names were removed). (B) RT-PCR was performed to analyze MEG3 lncRNA precipitated from RIP experiment with a CTCF antibody, IgG antibody was used as a control. (C) RNA-FISH was used to demonstrate the co-localization of the full-length MEG3 transcript and the CTCF-EGFP protein. (D) Western blot analysis was conducted on MDA-MB-231 cells following the knockdown of CTCF using two siRNAs to assess the impact on CXCR4 expression (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001). (E) Wound healing assay to evaluate the migratory capacity of MDA-MB-231 cells after the downregulation of CXCR4 caused by CTCF knockdown (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001) (the scale bar represents 500 μm). (F) Transwell assay for MDA-MB-231 after CTCF depletion (n = 3, ∗∗∗p < 0.001) (the scale bar represents 200 μm). (G&H) Wound healing assay and Transwell assay for MEG3 overexpression and CTCF depletion dual treatment (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significance) (the scale bar represents 500 μm for Wound healing assay and 200 μm for Transwell assay). (I) Western blot analysis showing the effect of MEG3 overexpression in combination with CTCF depletion on CXCR4 gene expression (n = 3, ∗∗∗p < 0.001, ns: no significance). (J) Western blot analysis showing the effect of dual overexpression of MEG3 and CTCF on CXCR4 gene expression (n = 3, ∗p < 0.05, ∗∗p < 0.01, ns: no significance).
    Primary Antibodies, supplied by EpiCypher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies/product/EpiCypher
    Average 93 stars, based on 1 article reviews
    primary antibodies - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    resource source identifier antibodies ctcf cutanatm cut run antibody  (EpiCypher)
    93
    EpiCypher resource source identifier antibodies ctcf cutanatm cut run antibody
    MEG3 interacts with <t>CTCF</t> to restrain the transcriptional activation on CXCR4. (A) Venn diagram illustrates the proteins detected in our MEG3 mass spectrometry data, predicted MEG3 binding proteins from the AnnoLnc2 database, CXCR4-ChIP-seq data from the Cistrome toolkit database, and data from genetic perturbation similarity analysis for CXCR4 upstream regulators from the GPSAdb (Duplicated gene names were removed). (B) RT-PCR was performed to analyze MEG3 lncRNA precipitated from RIP experiment with a CTCF antibody, IgG antibody was used as a control. (C) RNA-FISH was used to demonstrate the co-localization of the full-length MEG3 transcript and the CTCF-EGFP protein. (D) Western blot analysis was conducted on MDA-MB-231 cells following the knockdown of CTCF using two siRNAs to assess the impact on CXCR4 expression (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001). (E) Wound healing assay to evaluate the migratory capacity of MDA-MB-231 cells after the downregulation of CXCR4 caused by CTCF knockdown (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001) (the scale bar represents 500 μm). (F) Transwell assay for MDA-MB-231 after CTCF depletion (n = 3, ∗∗∗p < 0.001) (the scale bar represents 200 μm). (G&H) Wound healing assay and Transwell assay for MEG3 overexpression and CTCF depletion dual treatment (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significance) (the scale bar represents 500 μm for Wound healing assay and 200 μm for Transwell assay). (I) Western blot analysis showing the effect of MEG3 overexpression in combination with CTCF depletion on CXCR4 gene expression (n = 3, ∗∗∗p < 0.001, ns: no significance). (J) Western blot analysis showing the effect of dual overexpression of MEG3 and CTCF on CXCR4 gene expression (n = 3, ∗p < 0.05, ∗∗p < 0.01, ns: no significance).
    Resource Source Identifier Antibodies Ctcf Cutanatm Cut Run Antibody, supplied by EpiCypher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resource source identifier antibodies ctcf cutanatm cut run antibody/product/EpiCypher
    Average 93 stars, based on 1 article reviews
    resource source identifier antibodies ctcf cutanatm cut run antibody - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    rabbit anti ctcf  (Proteintech)
    93
    Proteintech rabbit anti ctcf
    MEG3 interacts with <t>CTCF</t> to restrain the transcriptional activation on CXCR4. (A) Venn diagram illustrates the proteins detected in our MEG3 mass spectrometry data, predicted MEG3 binding proteins from the AnnoLnc2 database, CXCR4-ChIP-seq data from the Cistrome toolkit database, and data from genetic perturbation similarity analysis for CXCR4 upstream regulators from the GPSAdb (Duplicated gene names were removed). (B) RT-PCR was performed to analyze MEG3 lncRNA precipitated from RIP experiment with a CTCF antibody, IgG antibody was used as a control. (C) RNA-FISH was used to demonstrate the co-localization of the full-length MEG3 transcript and the CTCF-EGFP protein. (D) Western blot analysis was conducted on MDA-MB-231 cells following the knockdown of CTCF using two siRNAs to assess the impact on CXCR4 expression (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001). (E) Wound healing assay to evaluate the migratory capacity of MDA-MB-231 cells after the downregulation of CXCR4 caused by CTCF knockdown (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001) (the scale bar represents 500 μm). (F) Transwell assay for MDA-MB-231 after CTCF depletion (n = 3, ∗∗∗p < 0.001) (the scale bar represents 200 μm). (G&H) Wound healing assay and Transwell assay for MEG3 overexpression and CTCF depletion dual treatment (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significance) (the scale bar represents 500 μm for Wound healing assay and 200 μm for Transwell assay). (I) Western blot analysis showing the effect of MEG3 overexpression in combination with CTCF depletion on CXCR4 gene expression (n = 3, ∗∗∗p < 0.001, ns: no significance). (J) Western blot analysis showing the effect of dual overexpression of MEG3 and CTCF on CXCR4 gene expression (n = 3, ∗p < 0.05, ∗∗p < 0.01, ns: no significance).
    Rabbit Anti Ctcf, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ctcf/product/Proteintech
    Average 93 stars, based on 1 article reviews
    rabbit anti ctcf - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    MEG3 interacts with CTCF to restrain the transcriptional activation on CXCR4. (A) Venn diagram illustrates the proteins detected in our MEG3 mass spectrometry data, predicted MEG3 binding proteins from the AnnoLnc2 database, CXCR4-ChIP-seq data from the Cistrome toolkit database, and data from genetic perturbation similarity analysis for CXCR4 upstream regulators from the GPSAdb (Duplicated gene names were removed). (B) RT-PCR was performed to analyze MEG3 lncRNA precipitated from RIP experiment with a CTCF antibody, IgG antibody was used as a control. (C) RNA-FISH was used to demonstrate the co-localization of the full-length MEG3 transcript and the CTCF-EGFP protein. (D) Western blot analysis was conducted on MDA-MB-231 cells following the knockdown of CTCF using two siRNAs to assess the impact on CXCR4 expression (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001). (E) Wound healing assay to evaluate the migratory capacity of MDA-MB-231 cells after the downregulation of CXCR4 caused by CTCF knockdown (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001) (the scale bar represents 500 μm). (F) Transwell assay for MDA-MB-231 after CTCF depletion (n = 3, ∗∗∗p < 0.001) (the scale bar represents 200 μm). (G&H) Wound healing assay and Transwell assay for MEG3 overexpression and CTCF depletion dual treatment (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significance) (the scale bar represents 500 μm for Wound healing assay and 200 μm for Transwell assay). (I) Western blot analysis showing the effect of MEG3 overexpression in combination with CTCF depletion on CXCR4 gene expression (n = 3, ∗∗∗p < 0.001, ns: no significance). (J) Western blot analysis showing the effect of dual overexpression of MEG3 and CTCF on CXCR4 gene expression (n = 3, ∗p < 0.05, ∗∗p < 0.01, ns: no significance).

    Journal: Non-coding RNA Research

    Article Title: LncRNA MEG3/CTCF-CXCR4 axis functions in the regulation of breast cancer cell migration

    doi: 10.1016/j.ncrna.2025.05.014

    Figure Lengend Snippet: MEG3 interacts with CTCF to restrain the transcriptional activation on CXCR4. (A) Venn diagram illustrates the proteins detected in our MEG3 mass spectrometry data, predicted MEG3 binding proteins from the AnnoLnc2 database, CXCR4-ChIP-seq data from the Cistrome toolkit database, and data from genetic perturbation similarity analysis for CXCR4 upstream regulators from the GPSAdb (Duplicated gene names were removed). (B) RT-PCR was performed to analyze MEG3 lncRNA precipitated from RIP experiment with a CTCF antibody, IgG antibody was used as a control. (C) RNA-FISH was used to demonstrate the co-localization of the full-length MEG3 transcript and the CTCF-EGFP protein. (D) Western blot analysis was conducted on MDA-MB-231 cells following the knockdown of CTCF using two siRNAs to assess the impact on CXCR4 expression (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001). (E) Wound healing assay to evaluate the migratory capacity of MDA-MB-231 cells after the downregulation of CXCR4 caused by CTCF knockdown (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001) (the scale bar represents 500 μm). (F) Transwell assay for MDA-MB-231 after CTCF depletion (n = 3, ∗∗∗p < 0.001) (the scale bar represents 200 μm). (G&H) Wound healing assay and Transwell assay for MEG3 overexpression and CTCF depletion dual treatment (n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significance) (the scale bar represents 500 μm for Wound healing assay and 200 μm for Transwell assay). (I) Western blot analysis showing the effect of MEG3 overexpression in combination with CTCF depletion on CXCR4 gene expression (n = 3, ∗∗∗p < 0.001, ns: no significance). (J) Western blot analysis showing the effect of dual overexpression of MEG3 and CTCF on CXCR4 gene expression (n = 3, ∗p < 0.05, ∗∗p < 0.01, ns: no significance).

    Article Snippet: Antibodies targeted to CTCF (Proteintech, 30428-1-AP, 1:1000), GAPDH (Proteintech, 10494-1-AP, 1:10000), MYH9 (Proteintech, 14844-1-AP, 1:500), PES1 (Proteintech, 13553-1-AP, 1:1000), IKBIP (Proteintech, 14589-1-AP, 1:500), PHB (Proteintech, 10787-1-AP, 1:1000) and CXCR4 (ABclonal, A19035, 1:1000) were used in Western blot.

    Techniques: Activation Assay, Mass Spectrometry, Binding Assay, ChIP-sequencing, Reverse Transcription Polymerase Chain Reaction, Control, Western Blot, Knockdown, Expressing, Wound Healing Assay, Transwell Assay, Over Expression, Gene Expression