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murine anti nuclear antibody igg elisa kit  (Cusabio)


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    Cusabio murine anti nuclear antibody igg elisa kit
    GPR183 antagonism suppresses nephritis severity, plasmablast differentiation, and autoantibody production in female mice (A) Line graph shows urine proteinuria score in NIBR189-treated ( n = 14) compared to control ( n = 17) female mice with lupus nephritis. (B) Representative images (left, scale bar = 100 μm) and summary dot plot (right) show histological scoring of kidney pathology in NIBR189-treated ( n = 11) compared to controls ( n = 13) female mice with lupus nephritis. (C) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of splenic CD19 + CD95 + GL7 + Germinal Center (GC) B cells in NIBR189-treated ( n = 14) and control ( n = 18) female mice with lupus nephritis. (D) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of CD19 + CD138 + Blimp-1 + plasmablasts in NIBR189-treated ( n = 14) and control ( n = 17) female mice with lupus nephritis. (E) Summary dot plot shows the abundance of total IgG (μg/mL) in the sera of NIBR189-treated ( n = 7) and control ( n = 8) female mice with lupus nephritis. (F) Summary dot plot shows the abundance <t>of</t> <t>anti-nuclear</t> antibodies (μg/mL) in the sera of NIBR189-treated ( n = 11) and control ( n = 14) female mice with lupus nephritis. Lines in line graphs and dot plots represent mean ± SEM. Cumulative data of at least 3 independent experiments is shown. A two-way ANOVA test and B–F Mann-Whitney test were used to determine the significance of the difference between groups.
    Murine Anti Nuclear Antibody Igg Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/csb+e12912m/Mouse+anti-nuclear+Antibody+(IgG)+ELISA+Kit/pmc12972708-55-0-7
    Average 93 stars, based on 29 article reviews
    murine anti nuclear antibody igg elisa kit - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus"

    Article Title: Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus

    Journal: iScience

    doi: 10.1016/j.isci.2026.114980

    GPR183 antagonism suppresses nephritis severity, plasmablast differentiation, and autoantibody production in female mice (A) Line graph shows urine proteinuria score in NIBR189-treated ( n = 14) compared to control ( n = 17) female mice with lupus nephritis. (B) Representative images (left, scale bar = 100 μm) and summary dot plot (right) show histological scoring of kidney pathology in NIBR189-treated ( n = 11) compared to controls ( n = 13) female mice with lupus nephritis. (C) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of splenic CD19 + CD95 + GL7 + Germinal Center (GC) B cells in NIBR189-treated ( n = 14) and control ( n = 18) female mice with lupus nephritis. (D) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of CD19 + CD138 + Blimp-1 + plasmablasts in NIBR189-treated ( n = 14) and control ( n = 17) female mice with lupus nephritis. (E) Summary dot plot shows the abundance of total IgG (μg/mL) in the sera of NIBR189-treated ( n = 7) and control ( n = 8) female mice with lupus nephritis. (F) Summary dot plot shows the abundance of anti-nuclear antibodies (μg/mL) in the sera of NIBR189-treated ( n = 11) and control ( n = 14) female mice with lupus nephritis. Lines in line graphs and dot plots represent mean ± SEM. Cumulative data of at least 3 independent experiments is shown. A two-way ANOVA test and B–F Mann-Whitney test were used to determine the significance of the difference between groups.
    Figure Legend Snippet: GPR183 antagonism suppresses nephritis severity, plasmablast differentiation, and autoantibody production in female mice (A) Line graph shows urine proteinuria score in NIBR189-treated ( n = 14) compared to control ( n = 17) female mice with lupus nephritis. (B) Representative images (left, scale bar = 100 μm) and summary dot plot (right) show histological scoring of kidney pathology in NIBR189-treated ( n = 11) compared to controls ( n = 13) female mice with lupus nephritis. (C) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of splenic CD19 + CD95 + GL7 + Germinal Center (GC) B cells in NIBR189-treated ( n = 14) and control ( n = 18) female mice with lupus nephritis. (D) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of CD19 + CD138 + Blimp-1 + plasmablasts in NIBR189-treated ( n = 14) and control ( n = 17) female mice with lupus nephritis. (E) Summary dot plot shows the abundance of total IgG (μg/mL) in the sera of NIBR189-treated ( n = 7) and control ( n = 8) female mice with lupus nephritis. (F) Summary dot plot shows the abundance of anti-nuclear antibodies (μg/mL) in the sera of NIBR189-treated ( n = 11) and control ( n = 14) female mice with lupus nephritis. Lines in line graphs and dot plots represent mean ± SEM. Cumulative data of at least 3 independent experiments is shown. A two-way ANOVA test and B–F Mann-Whitney test were used to determine the significance of the difference between groups.

    Techniques Used: Control, Flow Cytometry, MANN-WHITNEY

    GPR183 antagonism does not suppress nephritis severity, plasmablast differentiation, and autoantibody production in male mice (A) Line graph shows urine proteinuria score in NIBR189-treated ( n = 14) compared to control ( n = 23) male mice with lupus nephritis. (B) Representative images (left, scale bar = 100 μm) and summary dot plot (right) show histological scoring of kidney pathology in NIBR189-treated ( n = 9) compared to controls ( n = 14) male mice with lupus nephritis. (C) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of splenic CD19 + CD95 + GL7 + Germinal Center (GC) B cells in NIBR189-treated ( n = 11) and control ( n = 17) male mice with lupus nephritis. (D) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of CD19 + CD138 + Blimp-1 + plasmablasts in NIBR189-treated ( n = 13) and control ( n = 20) male mice with lupus nephritis. (E) Summary dot plot shows abundance of total IgG (μg/mL) in the sera of NIBR189-treated ( n = 7) and control ( n = 8) male mice with lupus nephritis. (F) Summary dot plot shows abundance of anti-nuclear antibodies (μg/mL) in the sera of NIBR189-treated ( n = 11) and control ( n = 18) male mice with lupus nephritis. Lines in line graphs and dot plots represent mean ± SEM. Cumulative data of at least 3 independent experiments is shown. A two-way ANOVA test and B–F Mann-Whitney test were used to determine the significance of the difference between groups.
    Figure Legend Snippet: GPR183 antagonism does not suppress nephritis severity, plasmablast differentiation, and autoantibody production in male mice (A) Line graph shows urine proteinuria score in NIBR189-treated ( n = 14) compared to control ( n = 23) male mice with lupus nephritis. (B) Representative images (left, scale bar = 100 μm) and summary dot plot (right) show histological scoring of kidney pathology in NIBR189-treated ( n = 9) compared to controls ( n = 14) male mice with lupus nephritis. (C) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of splenic CD19 + CD95 + GL7 + Germinal Center (GC) B cells in NIBR189-treated ( n = 11) and control ( n = 17) male mice with lupus nephritis. (D) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of CD19 + CD138 + Blimp-1 + plasmablasts in NIBR189-treated ( n = 13) and control ( n = 20) male mice with lupus nephritis. (E) Summary dot plot shows abundance of total IgG (μg/mL) in the sera of NIBR189-treated ( n = 7) and control ( n = 8) male mice with lupus nephritis. (F) Summary dot plot shows abundance of anti-nuclear antibodies (μg/mL) in the sera of NIBR189-treated ( n = 11) and control ( n = 18) male mice with lupus nephritis. Lines in line graphs and dot plots represent mean ± SEM. Cumulative data of at least 3 independent experiments is shown. A two-way ANOVA test and B–F Mann-Whitney test were used to determine the significance of the difference between groups.

    Techniques Used: Control, Flow Cytometry, MANN-WHITNEY

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Microbiota‐Derived Inosine Suppresses Systemic Autoimmunity via Restriction of B Cell Differentiation and Migration
    Article Snippet: Mouse anti‐double stranded DNA antibody (IgG) ELISA Kit , CUSABIO , CSB‐E11194m. .. Mouse ANA (IgG) ELISA Kit , CUSABIO , CSB‐E12912m. .. Precision Plus Protein Dual Color Standards , BIO‐RAD , 1610394.

    Article Title: Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Picopure TM RNA isolation kit ThermoFisher Scientific Cat# KIT0204 iScript TM cDNA synthesis kit Biorad Cat# 1708891 iQ TM SYBR® green supermix Biorad Cat# 1708882 NEBNext® Single Cell/Low Input RNA Library Prep Kit Illumina Cat# E6420S Murine IgG ELISA kit ThermoFisher Scientific Cat# 88-50470-88 Murine anti-nuclear antibody (IgG) ELISA kit Cusabio Cat# CSB-E12912m Deposited data JSLE and HC GPR183 + CD27 + B cell bulk RNAseq This paper NCBI Sequence Read Archive: PRJNA1248172 Experimental models: Organisms/strains Mouse, C57BL/6 Envigo (UK) or Charles River (UK) N/A Mouse, DBA/2 Envigo (UK) or Charles River (France/Netherlands) N/A Oligonucleotides Human GPR183 F Thermo 5 ′ -GAATCGGAGATGCCTTGTGT-3 ′ Human GPR183 R – 5 ′ - GCCTCCTGCTTTGACATAGG-3 ′ Human 18S F – 5 ′ -CTTATTAACGTTAGGGGCTA-3 ′ Human 18S R – 5 ′ -GCTAACCTACCAAATCACTC-3 ′ Software and algorithms NDP.view2 Image viewing software Hamamatsu https://www.hamamatsu.com/eu/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html FlowJo 10 v9 or v10 FlowJo https://www.flowjo.com/flowjo/download BCL Convert Software v3.7.5 Illumina https://emea.support.illumina.com/sequencing/ sequencing_software/bcl-convert/downloads.html R Studio version 4.4 R https://cran.r-project.org/bin/macosx/ RNA-STAR Encode https://www.encodeproject.org/software/star/ Fiji (ImageJ) ImageJ https://imagej.net/software/fiji/downloads ZEISS ZEN 3.9 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen-lite.html GraphPad Prism version 9 or 10 GraphPad https://www.graphpad.com Adobe Illustrator Adobe https://www.adobe.com/uk/products/illustrator BioRender BioRender https://www.biorender.com Other RPMI-1640 media Sigma-Aldrich Cat# R8758 Foetal bovine serum Cytiva Cat# SV30160.03 Penicillin/Streptomycin Sigma-Aldrich Cat# P0781 Ethylenediaminetetraacetic acid ThermoFisher Scientific Cat# AM9260G Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 Hanks balanced salt solution Gibco Cat# 14175-053 Neutral buffered formalin Scytek Cat# FRN999 LIVE/DEAD Fixable Blue Dead Cell Stain Invitrogen/Thermo Cat# L34961 Lightning Link Conjugation Kit - Alexa Fluor 700 Abcam Cat# Ab269824 Oligo dT beads New England Biolabs Cat# S1419S Normal Goat Serum Sigma-Aldrich Cat# G9023 Brilliant stain buffer BD Biosciences Cat# 563794 M. tuberculosis H37 Ra, desiccated BD Biosciences Cat# 231141 (Continued on next page) iScience 29, 114980, March 20, 2026 e2 ..

    Article Title: Single-cell analyses of intestinal epithelium reveal the dysregulation of gut immune microenvironment in systemic lupus erythematosus
    Article Snippet: .. Serum levels of ANA were measured using an ELISA kit from CUSABIO (CSB-E12912m). .. Quantitative analysis of proteinuria was performed using a Bradford assay kit acquired from Nanjing Jiancheng Bioengineering Institute (C035-2-1).

    Isolation:

    Article Title: Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Picopure TM RNA isolation kit ThermoFisher Scientific Cat# KIT0204 iScript TM cDNA synthesis kit Biorad Cat# 1708891 iQ TM SYBR® green supermix Biorad Cat# 1708882 NEBNext® Single Cell/Low Input RNA Library Prep Kit Illumina Cat# E6420S Murine IgG ELISA kit ThermoFisher Scientific Cat# 88-50470-88 Murine anti-nuclear antibody (IgG) ELISA kit Cusabio Cat# CSB-E12912m Deposited data JSLE and HC GPR183 + CD27 + B cell bulk RNAseq This paper NCBI Sequence Read Archive: PRJNA1248172 Experimental models: Organisms/strains Mouse, C57BL/6 Envigo (UK) or Charles River (UK) N/A Mouse, DBA/2 Envigo (UK) or Charles River (France/Netherlands) N/A Oligonucleotides Human GPR183 F Thermo 5 ′ -GAATCGGAGATGCCTTGTGT-3 ′ Human GPR183 R – 5 ′ - GCCTCCTGCTTTGACATAGG-3 ′ Human 18S F – 5 ′ -CTTATTAACGTTAGGGGCTA-3 ′ Human 18S R – 5 ′ -GCTAACCTACCAAATCACTC-3 ′ Software and algorithms NDP.view2 Image viewing software Hamamatsu https://www.hamamatsu.com/eu/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html FlowJo 10 v9 or v10 FlowJo https://www.flowjo.com/flowjo/download BCL Convert Software v3.7.5 Illumina https://emea.support.illumina.com/sequencing/ sequencing_software/bcl-convert/downloads.html R Studio version 4.4 R https://cran.r-project.org/bin/macosx/ RNA-STAR Encode https://www.encodeproject.org/software/star/ Fiji (ImageJ) ImageJ https://imagej.net/software/fiji/downloads ZEISS ZEN 3.9 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen-lite.html GraphPad Prism version 9 or 10 GraphPad https://www.graphpad.com Adobe Illustrator Adobe https://www.adobe.com/uk/products/illustrator BioRender BioRender https://www.biorender.com Other RPMI-1640 media Sigma-Aldrich Cat# R8758 Foetal bovine serum Cytiva Cat# SV30160.03 Penicillin/Streptomycin Sigma-Aldrich Cat# P0781 Ethylenediaminetetraacetic acid ThermoFisher Scientific Cat# AM9260G Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 Hanks balanced salt solution Gibco Cat# 14175-053 Neutral buffered formalin Scytek Cat# FRN999 LIVE/DEAD Fixable Blue Dead Cell Stain Invitrogen/Thermo Cat# L34961 Lightning Link Conjugation Kit - Alexa Fluor 700 Abcam Cat# Ab269824 Oligo dT beads New England Biolabs Cat# S1419S Normal Goat Serum Sigma-Aldrich Cat# G9023 Brilliant stain buffer BD Biosciences Cat# 563794 M. tuberculosis H37 Ra, desiccated BD Biosciences Cat# 231141 (Continued on next page) iScience 29, 114980, March 20, 2026 e2 ..

    cDNA Synthesis:

    Article Title: Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Picopure TM RNA isolation kit ThermoFisher Scientific Cat# KIT0204 iScript TM cDNA synthesis kit Biorad Cat# 1708891 iQ TM SYBR® green supermix Biorad Cat# 1708882 NEBNext® Single Cell/Low Input RNA Library Prep Kit Illumina Cat# E6420S Murine IgG ELISA kit ThermoFisher Scientific Cat# 88-50470-88 Murine anti-nuclear antibody (IgG) ELISA kit Cusabio Cat# CSB-E12912m Deposited data JSLE and HC GPR183 + CD27 + B cell bulk RNAseq This paper NCBI Sequence Read Archive: PRJNA1248172 Experimental models: Organisms/strains Mouse, C57BL/6 Envigo (UK) or Charles River (UK) N/A Mouse, DBA/2 Envigo (UK) or Charles River (France/Netherlands) N/A Oligonucleotides Human GPR183 F Thermo 5 ′ -GAATCGGAGATGCCTTGTGT-3 ′ Human GPR183 R – 5 ′ - GCCTCCTGCTTTGACATAGG-3 ′ Human 18S F – 5 ′ -CTTATTAACGTTAGGGGCTA-3 ′ Human 18S R – 5 ′ -GCTAACCTACCAAATCACTC-3 ′ Software and algorithms NDP.view2 Image viewing software Hamamatsu https://www.hamamatsu.com/eu/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html FlowJo 10 v9 or v10 FlowJo https://www.flowjo.com/flowjo/download BCL Convert Software v3.7.5 Illumina https://emea.support.illumina.com/sequencing/ sequencing_software/bcl-convert/downloads.html R Studio version 4.4 R https://cran.r-project.org/bin/macosx/ RNA-STAR Encode https://www.encodeproject.org/software/star/ Fiji (ImageJ) ImageJ https://imagej.net/software/fiji/downloads ZEISS ZEN 3.9 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen-lite.html GraphPad Prism version 9 or 10 GraphPad https://www.graphpad.com Adobe Illustrator Adobe https://www.adobe.com/uk/products/illustrator BioRender BioRender https://www.biorender.com Other RPMI-1640 media Sigma-Aldrich Cat# R8758 Foetal bovine serum Cytiva Cat# SV30160.03 Penicillin/Streptomycin Sigma-Aldrich Cat# P0781 Ethylenediaminetetraacetic acid ThermoFisher Scientific Cat# AM9260G Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 Hanks balanced salt solution Gibco Cat# 14175-053 Neutral buffered formalin Scytek Cat# FRN999 LIVE/DEAD Fixable Blue Dead Cell Stain Invitrogen/Thermo Cat# L34961 Lightning Link Conjugation Kit - Alexa Fluor 700 Abcam Cat# Ab269824 Oligo dT beads New England Biolabs Cat# S1419S Normal Goat Serum Sigma-Aldrich Cat# G9023 Brilliant stain buffer BD Biosciences Cat# 563794 M. tuberculosis H37 Ra, desiccated BD Biosciences Cat# 231141 (Continued on next page) iScience 29, 114980, March 20, 2026 e2 ..

    SYBR Green Assay:

    Article Title: Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Picopure TM RNA isolation kit ThermoFisher Scientific Cat# KIT0204 iScript TM cDNA synthesis kit Biorad Cat# 1708891 iQ TM SYBR® green supermix Biorad Cat# 1708882 NEBNext® Single Cell/Low Input RNA Library Prep Kit Illumina Cat# E6420S Murine IgG ELISA kit ThermoFisher Scientific Cat# 88-50470-88 Murine anti-nuclear antibody (IgG) ELISA kit Cusabio Cat# CSB-E12912m Deposited data JSLE and HC GPR183 + CD27 + B cell bulk RNAseq This paper NCBI Sequence Read Archive: PRJNA1248172 Experimental models: Organisms/strains Mouse, C57BL/6 Envigo (UK) or Charles River (UK) N/A Mouse, DBA/2 Envigo (UK) or Charles River (France/Netherlands) N/A Oligonucleotides Human GPR183 F Thermo 5 ′ -GAATCGGAGATGCCTTGTGT-3 ′ Human GPR183 R – 5 ′ - GCCTCCTGCTTTGACATAGG-3 ′ Human 18S F – 5 ′ -CTTATTAACGTTAGGGGCTA-3 ′ Human 18S R – 5 ′ -GCTAACCTACCAAATCACTC-3 ′ Software and algorithms NDP.view2 Image viewing software Hamamatsu https://www.hamamatsu.com/eu/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html FlowJo 10 v9 or v10 FlowJo https://www.flowjo.com/flowjo/download BCL Convert Software v3.7.5 Illumina https://emea.support.illumina.com/sequencing/ sequencing_software/bcl-convert/downloads.html R Studio version 4.4 R https://cran.r-project.org/bin/macosx/ RNA-STAR Encode https://www.encodeproject.org/software/star/ Fiji (ImageJ) ImageJ https://imagej.net/software/fiji/downloads ZEISS ZEN 3.9 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen-lite.html GraphPad Prism version 9 or 10 GraphPad https://www.graphpad.com Adobe Illustrator Adobe https://www.adobe.com/uk/products/illustrator BioRender BioRender https://www.biorender.com Other RPMI-1640 media Sigma-Aldrich Cat# R8758 Foetal bovine serum Cytiva Cat# SV30160.03 Penicillin/Streptomycin Sigma-Aldrich Cat# P0781 Ethylenediaminetetraacetic acid ThermoFisher Scientific Cat# AM9260G Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 Hanks balanced salt solution Gibco Cat# 14175-053 Neutral buffered formalin Scytek Cat# FRN999 LIVE/DEAD Fixable Blue Dead Cell Stain Invitrogen/Thermo Cat# L34961 Lightning Link Conjugation Kit - Alexa Fluor 700 Abcam Cat# Ab269824 Oligo dT beads New England Biolabs Cat# S1419S Normal Goat Serum Sigma-Aldrich Cat# G9023 Brilliant stain buffer BD Biosciences Cat# 563794 M. tuberculosis H37 Ra, desiccated BD Biosciences Cat# 231141 (Continued on next page) iScience 29, 114980, March 20, 2026 e2 ..

    RNA sequencing:

    Article Title: Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Picopure TM RNA isolation kit ThermoFisher Scientific Cat# KIT0204 iScript TM cDNA synthesis kit Biorad Cat# 1708891 iQ TM SYBR® green supermix Biorad Cat# 1708882 NEBNext® Single Cell/Low Input RNA Library Prep Kit Illumina Cat# E6420S Murine IgG ELISA kit ThermoFisher Scientific Cat# 88-50470-88 Murine anti-nuclear antibody (IgG) ELISA kit Cusabio Cat# CSB-E12912m Deposited data JSLE and HC GPR183 + CD27 + B cell bulk RNAseq This paper NCBI Sequence Read Archive: PRJNA1248172 Experimental models: Organisms/strains Mouse, C57BL/6 Envigo (UK) or Charles River (UK) N/A Mouse, DBA/2 Envigo (UK) or Charles River (France/Netherlands) N/A Oligonucleotides Human GPR183 F Thermo 5 ′ -GAATCGGAGATGCCTTGTGT-3 ′ Human GPR183 R – 5 ′ - GCCTCCTGCTTTGACATAGG-3 ′ Human 18S F – 5 ′ -CTTATTAACGTTAGGGGCTA-3 ′ Human 18S R – 5 ′ -GCTAACCTACCAAATCACTC-3 ′ Software and algorithms NDP.view2 Image viewing software Hamamatsu https://www.hamamatsu.com/eu/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html FlowJo 10 v9 or v10 FlowJo https://www.flowjo.com/flowjo/download BCL Convert Software v3.7.5 Illumina https://emea.support.illumina.com/sequencing/ sequencing_software/bcl-convert/downloads.html R Studio version 4.4 R https://cran.r-project.org/bin/macosx/ RNA-STAR Encode https://www.encodeproject.org/software/star/ Fiji (ImageJ) ImageJ https://imagej.net/software/fiji/downloads ZEISS ZEN 3.9 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen-lite.html GraphPad Prism version 9 or 10 GraphPad https://www.graphpad.com Adobe Illustrator Adobe https://www.adobe.com/uk/products/illustrator BioRender BioRender https://www.biorender.com Other RPMI-1640 media Sigma-Aldrich Cat# R8758 Foetal bovine serum Cytiva Cat# SV30160.03 Penicillin/Streptomycin Sigma-Aldrich Cat# P0781 Ethylenediaminetetraacetic acid ThermoFisher Scientific Cat# AM9260G Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 Hanks balanced salt solution Gibco Cat# 14175-053 Neutral buffered formalin Scytek Cat# FRN999 LIVE/DEAD Fixable Blue Dead Cell Stain Invitrogen/Thermo Cat# L34961 Lightning Link Conjugation Kit - Alexa Fluor 700 Abcam Cat# Ab269824 Oligo dT beads New England Biolabs Cat# S1419S Normal Goat Serum Sigma-Aldrich Cat# G9023 Brilliant stain buffer BD Biosciences Cat# 563794 M. tuberculosis H37 Ra, desiccated BD Biosciences Cat# 231141 (Continued on next page) iScience 29, 114980, March 20, 2026 e2 ..

    Sequencing:

    Article Title: Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Picopure TM RNA isolation kit ThermoFisher Scientific Cat# KIT0204 iScript TM cDNA synthesis kit Biorad Cat# 1708891 iQ TM SYBR® green supermix Biorad Cat# 1708882 NEBNext® Single Cell/Low Input RNA Library Prep Kit Illumina Cat# E6420S Murine IgG ELISA kit ThermoFisher Scientific Cat# 88-50470-88 Murine anti-nuclear antibody (IgG) ELISA kit Cusabio Cat# CSB-E12912m Deposited data JSLE and HC GPR183 + CD27 + B cell bulk RNAseq This paper NCBI Sequence Read Archive: PRJNA1248172 Experimental models: Organisms/strains Mouse, C57BL/6 Envigo (UK) or Charles River (UK) N/A Mouse, DBA/2 Envigo (UK) or Charles River (France/Netherlands) N/A Oligonucleotides Human GPR183 F Thermo 5 ′ -GAATCGGAGATGCCTTGTGT-3 ′ Human GPR183 R – 5 ′ - GCCTCCTGCTTTGACATAGG-3 ′ Human 18S F – 5 ′ -CTTATTAACGTTAGGGGCTA-3 ′ Human 18S R – 5 ′ -GCTAACCTACCAAATCACTC-3 ′ Software and algorithms NDP.view2 Image viewing software Hamamatsu https://www.hamamatsu.com/eu/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html FlowJo 10 v9 or v10 FlowJo https://www.flowjo.com/flowjo/download BCL Convert Software v3.7.5 Illumina https://emea.support.illumina.com/sequencing/ sequencing_software/bcl-convert/downloads.html R Studio version 4.4 R https://cran.r-project.org/bin/macosx/ RNA-STAR Encode https://www.encodeproject.org/software/star/ Fiji (ImageJ) ImageJ https://imagej.net/software/fiji/downloads ZEISS ZEN 3.9 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen-lite.html GraphPad Prism version 9 or 10 GraphPad https://www.graphpad.com Adobe Illustrator Adobe https://www.adobe.com/uk/products/illustrator BioRender BioRender https://www.biorender.com Other RPMI-1640 media Sigma-Aldrich Cat# R8758 Foetal bovine serum Cytiva Cat# SV30160.03 Penicillin/Streptomycin Sigma-Aldrich Cat# P0781 Ethylenediaminetetraacetic acid ThermoFisher Scientific Cat# AM9260G Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 Hanks balanced salt solution Gibco Cat# 14175-053 Neutral buffered formalin Scytek Cat# FRN999 LIVE/DEAD Fixable Blue Dead Cell Stain Invitrogen/Thermo Cat# L34961 Lightning Link Conjugation Kit - Alexa Fluor 700 Abcam Cat# Ab269824 Oligo dT beads New England Biolabs Cat# S1419S Normal Goat Serum Sigma-Aldrich Cat# G9023 Brilliant stain buffer BD Biosciences Cat# 563794 M. tuberculosis H37 Ra, desiccated BD Biosciences Cat# 231141 (Continued on next page) iScience 29, 114980, March 20, 2026 e2 ..

    Software:

    Article Title: Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Picopure TM RNA isolation kit ThermoFisher Scientific Cat# KIT0204 iScript TM cDNA synthesis kit Biorad Cat# 1708891 iQ TM SYBR® green supermix Biorad Cat# 1708882 NEBNext® Single Cell/Low Input RNA Library Prep Kit Illumina Cat# E6420S Murine IgG ELISA kit ThermoFisher Scientific Cat# 88-50470-88 Murine anti-nuclear antibody (IgG) ELISA kit Cusabio Cat# CSB-E12912m Deposited data JSLE and HC GPR183 + CD27 + B cell bulk RNAseq This paper NCBI Sequence Read Archive: PRJNA1248172 Experimental models: Organisms/strains Mouse, C57BL/6 Envigo (UK) or Charles River (UK) N/A Mouse, DBA/2 Envigo (UK) or Charles River (France/Netherlands) N/A Oligonucleotides Human GPR183 F Thermo 5 ′ -GAATCGGAGATGCCTTGTGT-3 ′ Human GPR183 R – 5 ′ - GCCTCCTGCTTTGACATAGG-3 ′ Human 18S F – 5 ′ -CTTATTAACGTTAGGGGCTA-3 ′ Human 18S R – 5 ′ -GCTAACCTACCAAATCACTC-3 ′ Software and algorithms NDP.view2 Image viewing software Hamamatsu https://www.hamamatsu.com/eu/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html FlowJo 10 v9 or v10 FlowJo https://www.flowjo.com/flowjo/download BCL Convert Software v3.7.5 Illumina https://emea.support.illumina.com/sequencing/ sequencing_software/bcl-convert/downloads.html R Studio version 4.4 R https://cran.r-project.org/bin/macosx/ RNA-STAR Encode https://www.encodeproject.org/software/star/ Fiji (ImageJ) ImageJ https://imagej.net/software/fiji/downloads ZEISS ZEN 3.9 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen-lite.html GraphPad Prism version 9 or 10 GraphPad https://www.graphpad.com Adobe Illustrator Adobe https://www.adobe.com/uk/products/illustrator BioRender BioRender https://www.biorender.com Other RPMI-1640 media Sigma-Aldrich Cat# R8758 Foetal bovine serum Cytiva Cat# SV30160.03 Penicillin/Streptomycin Sigma-Aldrich Cat# P0781 Ethylenediaminetetraacetic acid ThermoFisher Scientific Cat# AM9260G Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 Hanks balanced salt solution Gibco Cat# 14175-053 Neutral buffered formalin Scytek Cat# FRN999 LIVE/DEAD Fixable Blue Dead Cell Stain Invitrogen/Thermo Cat# L34961 Lightning Link Conjugation Kit - Alexa Fluor 700 Abcam Cat# Ab269824 Oligo dT beads New England Biolabs Cat# S1419S Normal Goat Serum Sigma-Aldrich Cat# G9023 Brilliant stain buffer BD Biosciences Cat# 563794 M. tuberculosis H37 Ra, desiccated BD Biosciences Cat# 231141 (Continued on next page) iScience 29, 114980, March 20, 2026 e2 ..

    Red Blood Cell Lysis:

    Article Title: Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Picopure TM RNA isolation kit ThermoFisher Scientific Cat# KIT0204 iScript TM cDNA synthesis kit Biorad Cat# 1708891 iQ TM SYBR® green supermix Biorad Cat# 1708882 NEBNext® Single Cell/Low Input RNA Library Prep Kit Illumina Cat# E6420S Murine IgG ELISA kit ThermoFisher Scientific Cat# 88-50470-88 Murine anti-nuclear antibody (IgG) ELISA kit Cusabio Cat# CSB-E12912m Deposited data JSLE and HC GPR183 + CD27 + B cell bulk RNAseq This paper NCBI Sequence Read Archive: PRJNA1248172 Experimental models: Organisms/strains Mouse, C57BL/6 Envigo (UK) or Charles River (UK) N/A Mouse, DBA/2 Envigo (UK) or Charles River (France/Netherlands) N/A Oligonucleotides Human GPR183 F Thermo 5 ′ -GAATCGGAGATGCCTTGTGT-3 ′ Human GPR183 R – 5 ′ - GCCTCCTGCTTTGACATAGG-3 ′ Human 18S F – 5 ′ -CTTATTAACGTTAGGGGCTA-3 ′ Human 18S R – 5 ′ -GCTAACCTACCAAATCACTC-3 ′ Software and algorithms NDP.view2 Image viewing software Hamamatsu https://www.hamamatsu.com/eu/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html FlowJo 10 v9 or v10 FlowJo https://www.flowjo.com/flowjo/download BCL Convert Software v3.7.5 Illumina https://emea.support.illumina.com/sequencing/ sequencing_software/bcl-convert/downloads.html R Studio version 4.4 R https://cran.r-project.org/bin/macosx/ RNA-STAR Encode https://www.encodeproject.org/software/star/ Fiji (ImageJ) ImageJ https://imagej.net/software/fiji/downloads ZEISS ZEN 3.9 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen-lite.html GraphPad Prism version 9 or 10 GraphPad https://www.graphpad.com Adobe Illustrator Adobe https://www.adobe.com/uk/products/illustrator BioRender BioRender https://www.biorender.com Other RPMI-1640 media Sigma-Aldrich Cat# R8758 Foetal bovine serum Cytiva Cat# SV30160.03 Penicillin/Streptomycin Sigma-Aldrich Cat# P0781 Ethylenediaminetetraacetic acid ThermoFisher Scientific Cat# AM9260G Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 Hanks balanced salt solution Gibco Cat# 14175-053 Neutral buffered formalin Scytek Cat# FRN999 LIVE/DEAD Fixable Blue Dead Cell Stain Invitrogen/Thermo Cat# L34961 Lightning Link Conjugation Kit - Alexa Fluor 700 Abcam Cat# Ab269824 Oligo dT beads New England Biolabs Cat# S1419S Normal Goat Serum Sigma-Aldrich Cat# G9023 Brilliant stain buffer BD Biosciences Cat# 563794 M. tuberculosis H37 Ra, desiccated BD Biosciences Cat# 231141 (Continued on next page) iScience 29, 114980, March 20, 2026 e2 ..

    Staining:

    Article Title: Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Picopure TM RNA isolation kit ThermoFisher Scientific Cat# KIT0204 iScript TM cDNA synthesis kit Biorad Cat# 1708891 iQ TM SYBR® green supermix Biorad Cat# 1708882 NEBNext® Single Cell/Low Input RNA Library Prep Kit Illumina Cat# E6420S Murine IgG ELISA kit ThermoFisher Scientific Cat# 88-50470-88 Murine anti-nuclear antibody (IgG) ELISA kit Cusabio Cat# CSB-E12912m Deposited data JSLE and HC GPR183 + CD27 + B cell bulk RNAseq This paper NCBI Sequence Read Archive: PRJNA1248172 Experimental models: Organisms/strains Mouse, C57BL/6 Envigo (UK) or Charles River (UK) N/A Mouse, DBA/2 Envigo (UK) or Charles River (France/Netherlands) N/A Oligonucleotides Human GPR183 F Thermo 5 ′ -GAATCGGAGATGCCTTGTGT-3 ′ Human GPR183 R – 5 ′ - GCCTCCTGCTTTGACATAGG-3 ′ Human 18S F – 5 ′ -CTTATTAACGTTAGGGGCTA-3 ′ Human 18S R – 5 ′ -GCTAACCTACCAAATCACTC-3 ′ Software and algorithms NDP.view2 Image viewing software Hamamatsu https://www.hamamatsu.com/eu/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html FlowJo 10 v9 or v10 FlowJo https://www.flowjo.com/flowjo/download BCL Convert Software v3.7.5 Illumina https://emea.support.illumina.com/sequencing/ sequencing_software/bcl-convert/downloads.html R Studio version 4.4 R https://cran.r-project.org/bin/macosx/ RNA-STAR Encode https://www.encodeproject.org/software/star/ Fiji (ImageJ) ImageJ https://imagej.net/software/fiji/downloads ZEISS ZEN 3.9 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen-lite.html GraphPad Prism version 9 or 10 GraphPad https://www.graphpad.com Adobe Illustrator Adobe https://www.adobe.com/uk/products/illustrator BioRender BioRender https://www.biorender.com Other RPMI-1640 media Sigma-Aldrich Cat# R8758 Foetal bovine serum Cytiva Cat# SV30160.03 Penicillin/Streptomycin Sigma-Aldrich Cat# P0781 Ethylenediaminetetraacetic acid ThermoFisher Scientific Cat# AM9260G Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 Hanks balanced salt solution Gibco Cat# 14175-053 Neutral buffered formalin Scytek Cat# FRN999 LIVE/DEAD Fixable Blue Dead Cell Stain Invitrogen/Thermo Cat# L34961 Lightning Link Conjugation Kit - Alexa Fluor 700 Abcam Cat# Ab269824 Oligo dT beads New England Biolabs Cat# S1419S Normal Goat Serum Sigma-Aldrich Cat# G9023 Brilliant stain buffer BD Biosciences Cat# 563794 M. tuberculosis H37 Ra, desiccated BD Biosciences Cat# 231141 (Continued on next page) iScience 29, 114980, March 20, 2026 e2 ..

    Conjugation Assay:

    Article Title: Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Picopure TM RNA isolation kit ThermoFisher Scientific Cat# KIT0204 iScript TM cDNA synthesis kit Biorad Cat# 1708891 iQ TM SYBR® green supermix Biorad Cat# 1708882 NEBNext® Single Cell/Low Input RNA Library Prep Kit Illumina Cat# E6420S Murine IgG ELISA kit ThermoFisher Scientific Cat# 88-50470-88 Murine anti-nuclear antibody (IgG) ELISA kit Cusabio Cat# CSB-E12912m Deposited data JSLE and HC GPR183 + CD27 + B cell bulk RNAseq This paper NCBI Sequence Read Archive: PRJNA1248172 Experimental models: Organisms/strains Mouse, C57BL/6 Envigo (UK) or Charles River (UK) N/A Mouse, DBA/2 Envigo (UK) or Charles River (France/Netherlands) N/A Oligonucleotides Human GPR183 F Thermo 5 ′ -GAATCGGAGATGCCTTGTGT-3 ′ Human GPR183 R – 5 ′ - GCCTCCTGCTTTGACATAGG-3 ′ Human 18S F – 5 ′ -CTTATTAACGTTAGGGGCTA-3 ′ Human 18S R – 5 ′ -GCTAACCTACCAAATCACTC-3 ′ Software and algorithms NDP.view2 Image viewing software Hamamatsu https://www.hamamatsu.com/eu/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html FlowJo 10 v9 or v10 FlowJo https://www.flowjo.com/flowjo/download BCL Convert Software v3.7.5 Illumina https://emea.support.illumina.com/sequencing/ sequencing_software/bcl-convert/downloads.html R Studio version 4.4 R https://cran.r-project.org/bin/macosx/ RNA-STAR Encode https://www.encodeproject.org/software/star/ Fiji (ImageJ) ImageJ https://imagej.net/software/fiji/downloads ZEISS ZEN 3.9 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen-lite.html GraphPad Prism version 9 or 10 GraphPad https://www.graphpad.com Adobe Illustrator Adobe https://www.adobe.com/uk/products/illustrator BioRender BioRender https://www.biorender.com Other RPMI-1640 media Sigma-Aldrich Cat# R8758 Foetal bovine serum Cytiva Cat# SV30160.03 Penicillin/Streptomycin Sigma-Aldrich Cat# P0781 Ethylenediaminetetraacetic acid ThermoFisher Scientific Cat# AM9260G Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 Hanks balanced salt solution Gibco Cat# 14175-053 Neutral buffered formalin Scytek Cat# FRN999 LIVE/DEAD Fixable Blue Dead Cell Stain Invitrogen/Thermo Cat# L34961 Lightning Link Conjugation Kit - Alexa Fluor 700 Abcam Cat# Ab269824 Oligo dT beads New England Biolabs Cat# S1419S Normal Goat Serum Sigma-Aldrich Cat# G9023 Brilliant stain buffer BD Biosciences Cat# 563794 M. tuberculosis H37 Ra, desiccated BD Biosciences Cat# 231141 (Continued on next page) iScience 29, 114980, March 20, 2026 e2 ..



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    GPR183 antagonism suppresses nephritis severity, plasmablast differentiation, and autoantibody production in female mice (A) Line graph shows urine proteinuria score in NIBR189-treated ( n = 14) compared to control ( n = 17) female mice with lupus nephritis. (B) Representative images (left, scale bar = 100 μm) and summary dot plot (right) show histological scoring of kidney pathology in NIBR189-treated ( n = 11) compared to controls ( n = 13) female mice with lupus nephritis. (C) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of splenic CD19 + CD95 + GL7 + Germinal Center (GC) B cells in NIBR189-treated ( n = 14) and control ( n = 18) female mice with lupus nephritis. (D) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of CD19 + CD138 + Blimp-1 + plasmablasts in NIBR189-treated ( n = 14) and control ( n = 17) female mice with lupus nephritis. (E) Summary dot plot shows the abundance of total IgG (μg/mL) in the sera of NIBR189-treated ( n = 7) and control ( n = 8) female mice with lupus nephritis. (F) Summary dot plot shows the abundance of anti-nuclear antibodies (μg/mL) in the sera of NIBR189-treated ( n = 11) and control ( n = 14) female mice with lupus nephritis. Lines in line graphs and dot plots represent mean ± SEM. Cumulative data of at least 3 independent experiments is shown. A two-way ANOVA test and B–F Mann-Whitney test were used to determine the significance of the difference between groups.

    Journal: iScience

    Article Title: Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus

    doi: 10.1016/j.isci.2026.114980

    Figure Lengend Snippet: GPR183 antagonism suppresses nephritis severity, plasmablast differentiation, and autoantibody production in female mice (A) Line graph shows urine proteinuria score in NIBR189-treated ( n = 14) compared to control ( n = 17) female mice with lupus nephritis. (B) Representative images (left, scale bar = 100 μm) and summary dot plot (right) show histological scoring of kidney pathology in NIBR189-treated ( n = 11) compared to controls ( n = 13) female mice with lupus nephritis. (C) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of splenic CD19 + CD95 + GL7 + Germinal Center (GC) B cells in NIBR189-treated ( n = 14) and control ( n = 18) female mice with lupus nephritis. (D) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of CD19 + CD138 + Blimp-1 + plasmablasts in NIBR189-treated ( n = 14) and control ( n = 17) female mice with lupus nephritis. (E) Summary dot plot shows the abundance of total IgG (μg/mL) in the sera of NIBR189-treated ( n = 7) and control ( n = 8) female mice with lupus nephritis. (F) Summary dot plot shows the abundance of anti-nuclear antibodies (μg/mL) in the sera of NIBR189-treated ( n = 11) and control ( n = 14) female mice with lupus nephritis. Lines in line graphs and dot plots represent mean ± SEM. Cumulative data of at least 3 independent experiments is shown. A two-way ANOVA test and B–F Mann-Whitney test were used to determine the significance of the difference between groups.

    Article Snippet: Murine anti-nuclear antibody (IgG) ELISA kit , Cusabio , Cat# CSB-E12912m.

    Techniques: Control, Flow Cytometry, MANN-WHITNEY

    GPR183 antagonism does not suppress nephritis severity, plasmablast differentiation, and autoantibody production in male mice (A) Line graph shows urine proteinuria score in NIBR189-treated ( n = 14) compared to control ( n = 23) male mice with lupus nephritis. (B) Representative images (left, scale bar = 100 μm) and summary dot plot (right) show histological scoring of kidney pathology in NIBR189-treated ( n = 9) compared to controls ( n = 14) male mice with lupus nephritis. (C) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of splenic CD19 + CD95 + GL7 + Germinal Center (GC) B cells in NIBR189-treated ( n = 11) and control ( n = 17) male mice with lupus nephritis. (D) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of CD19 + CD138 + Blimp-1 + plasmablasts in NIBR189-treated ( n = 13) and control ( n = 20) male mice with lupus nephritis. (E) Summary dot plot shows abundance of total IgG (μg/mL) in the sera of NIBR189-treated ( n = 7) and control ( n = 8) male mice with lupus nephritis. (F) Summary dot plot shows abundance of anti-nuclear antibodies (μg/mL) in the sera of NIBR189-treated ( n = 11) and control ( n = 18) male mice with lupus nephritis. Lines in line graphs and dot plots represent mean ± SEM. Cumulative data of at least 3 independent experiments is shown. A two-way ANOVA test and B–F Mann-Whitney test were used to determine the significance of the difference between groups.

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    Article Title: Sexual dimorphism of early GPR183-dependent B cell responses in systemic lupus erythematosus

    doi: 10.1016/j.isci.2026.114980

    Figure Lengend Snippet: GPR183 antagonism does not suppress nephritis severity, plasmablast differentiation, and autoantibody production in male mice (A) Line graph shows urine proteinuria score in NIBR189-treated ( n = 14) compared to control ( n = 23) male mice with lupus nephritis. (B) Representative images (left, scale bar = 100 μm) and summary dot plot (right) show histological scoring of kidney pathology in NIBR189-treated ( n = 9) compared to controls ( n = 14) male mice with lupus nephritis. (C) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of splenic CD19 + CD95 + GL7 + Germinal Center (GC) B cells in NIBR189-treated ( n = 11) and control ( n = 17) male mice with lupus nephritis. (D) Representative flow cytometry plots (left) and summary dot plot (right) show the percentage of CD19 + CD138 + Blimp-1 + plasmablasts in NIBR189-treated ( n = 13) and control ( n = 20) male mice with lupus nephritis. (E) Summary dot plot shows abundance of total IgG (μg/mL) in the sera of NIBR189-treated ( n = 7) and control ( n = 8) male mice with lupus nephritis. (F) Summary dot plot shows abundance of anti-nuclear antibodies (μg/mL) in the sera of NIBR189-treated ( n = 11) and control ( n = 18) male mice with lupus nephritis. Lines in line graphs and dot plots represent mean ± SEM. Cumulative data of at least 3 independent experiments is shown. A two-way ANOVA test and B–F Mann-Whitney test were used to determine the significance of the difference between groups.

    Article Snippet: Murine anti-nuclear antibody (IgG) ELISA kit , Cusabio , Cat# CSB-E12912m.

    Techniques: Control, Flow Cytometry, MANN-WHITNEY