Journal: Sensors (Basel, Switzerland)

Article Title: Developing an Efficient and General Strategy for Immobilization of Small Molecules onto Microarrays Using Isocyanate Chemistry

doi: 10.3390/s16030378

Figure Lengend Snippet: ( a ) After reaction with anti-biotin antibody, the change in OI-RD image (differential OI-RD image) of a biotinylated compound microarray on hexyl-isocyanate functionalized slide with (PEG) 6 and catalyzed in pyridine vapor at room temperature; ( b ) Extracted immobilization efficiencies of five biotinylated compounds with different nucleophilic residues available for surface anchoring.

Article Snippet: Five biotinylated compounds, biotin-(PEG) 2 -NH 2 , biotin4-nitrophenyl ester, biotin-NH-NH 2 , biotin-(PEG) 6 -OH, and D-biotin (with intrinsic COOH group), were printed on as-prepared isocyanate functionalized glass slides with a contact microarray printer (SmartArrayer 136 Microarray Spotter, CapitalBio Corporation, Beijing, China).

Techniques: Microarray

Journal: Sensors (Basel, Switzerland)

Article Title: Developing an Efficient and General Strategy for Immobilization of Small Molecules onto Microarrays Using Isocyanate Chemistry

doi: 10.3390/s16030378

Figure Lengend Snippet: Combined auto-fluorescence/OI-RD image of a printed-and-washed compound microarray ( a ) on a hexyl-isocyanate functionalized slide and ( b ) on a phenyl-isocyanate functionalized slide.

Article Snippet: Five biotinylated compounds, biotin-(PEG) 2 -NH 2 , biotin4-nitrophenyl ester, biotin-NH-NH 2 , biotin-(PEG) 6 -OH, and D-biotin (with intrinsic COOH group), were printed on as-prepared isocyanate functionalized glass slides with a contact microarray printer (SmartArrayer 136 Microarray Spotter, CapitalBio Corporation, Beijing, China).

Techniques: Fluorescence, Microarray