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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
Computer Assisted Analysis, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer-assisted sperm analysis system sca® motility imaging software  (Microptic SL)
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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
Computer Assisted Sperm Analysis System Sca® Motility Imaging Software, supplied by Microptic SL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer-assisted sperm analysis system sca® motility imaging software - by Bioz Stars, 2026-07
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computer-assisted image analysis cognitionmaster software  (VMscope GmbH)
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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
Computer Assisted Image Analysis Cognitionmaster Software, supplied by VMscope GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer-assisted image analysis cognitionmaster software - by Bioz Stars, 2026-07
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computer assisted image analysis software  (Danaher Inc)
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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
Computer Assisted Image Analysis Software, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer assisted image analysis software - by Bioz Stars, 2026-07
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computer-assisted image analysis software halo  (Indica Labs)
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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
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nis-elements computer-assisted image analysis software  (Nikon)
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Nikon nis-elements computer-assisted image analysis software
Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
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computer-assisted image analysis software nis- elements  (Nikon)
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Nikon computer-assisted image analysis software nis- elements
Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
Computer Assisted Image Analysis Software Nis Elements, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer-assisted+image+analysis+software/pm37945550-63-34-41?v=Nikon
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computer-assisted image analysis software nis- elements - by Bioz Stars, 2026-07
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computer-assisted image analysis software  (Nikon)
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Nikon computer-assisted image analysis software
Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
Computer Assisted Image Analysis Software, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer-assisted+image+analysis+software/pm37061131-69-11-10?v=Nikon
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computer-assisted image analysis software - by Bioz Stars, 2026-07
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computer-assisted imaging analysis system genetools match software  (Syngene)
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Syngene computer-assisted imaging analysis system genetools match software
Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
Computer Assisted Imaging Analysis System Genetools Match Software, supplied by Syngene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer-assisted imaging analysis system genetools match software - by Bioz Stars, 2026-07
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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

Journal: Cells

Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

doi: 10.3390/cells15040360

Figure Lengend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

Article Snippet: The average size of metastases was quantified using computer-assisted analysis with CellSens Imaging software (Version 1.17; Olympus, Tokyo, Japan).

Techniques: Immunodetection, TUNEL Assay