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Isolation of individual DNA barcodes from the ClonTracer barcode virus library and workflow of barcoding and tracking PDOs within a mixture by both NGS and StarTrace PCR. Created in https://BioRender.com

Journal: bioRxiv

Article Title: StarTrace: A Multiplex Organoid Avatar Drug Testing Platform for Personalized Medicine

doi: 10.1101/2025.02.12.637574

Figure Lengend Snippet: Isolation of individual DNA barcodes from the ClonTracer barcode virus library and workflow of barcoding and tracking PDOs within a mixture by both NGS and StarTrace PCR. Created in https://BioRender.com

Article Snippet: We isolated 72 randomly picked barcode clones from a high-complexity ClonTracer barcoding library (addgene #67267) and generated infectious viral particles for tagging individual organoids ( ).

Techniques: Isolation, Virus