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Figure 3. Differentially expressed genes between A549‑P‑S and A549‑P cells by microarray assay and qPCR validation. (A) Each group in triplicate was shown on the same chip. Color intensity was assigned to ratios of gene expression; shades of red indicate genes that were upregulated; shades of green indicate genes that were downregulated. (B) 10 upregulated genes were identified by qPCR, which was consistent with cDNA microarray data. qPCR, quantitative polymerase chain reaction; <t>C3,</t> <t>complement</t> C3 precursor; C4b, complement 4B preproprotein; FGA, fibrinogen α chain precursor; FGB, fibrinogen β chain precursor; FGG, fibrinogen γ chain precursor; SELENBP1, selenium binding protein 1; TXNIP, thioredoxin-interacting protein; PDZK1IP1, PDZK-interacting protein 1; DUSP6, dual specificity protein phosphatase 6.
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Figure 3. Differentially expressed genes between A549‑P‑S and A549‑P cells by microarray assay and qPCR validation. (A) Each group in triplicate was shown on the same chip. Color intensity was assigned to ratios of gene expression; shades of red indicate genes that were upregulated; shades of green indicate genes that were downregulated. (B) 10 upregulated genes were identified by qPCR, which was consistent with cDNA microarray data. qPCR, quantitative polymerase chain reaction; C3, complement C3 precursor; C4b, complement 4B preproprotein; FGA, fibrinogen α chain precursor; FGB, fibrinogen β chain precursor; FGG, fibrinogen γ chain precursor; SELENBP1, selenium binding protein 1; TXNIP, thioredoxin-interacting protein; PDZK1IP1, PDZK-interacting protein 1; DUSP6, dual specificity protein phosphatase 6.

Journal: Molecular medicine reports

Article Title: Elevated expression of SLC34A2 inhibits the viability and invasion of A549 cells.

doi: 10.3892/mmr.2014.2376

Figure Lengend Snippet: Figure 3. Differentially expressed genes between A549‑P‑S and A549‑P cells by microarray assay and qPCR validation. (A) Each group in triplicate was shown on the same chip. Color intensity was assigned to ratios of gene expression; shades of red indicate genes that were upregulated; shades of green indicate genes that were downregulated. (B) 10 upregulated genes were identified by qPCR, which was consistent with cDNA microarray data. qPCR, quantitative polymerase chain reaction; C3, complement C3 precursor; C4b, complement 4B preproprotein; FGA, fibrinogen α chain precursor; FGB, fibrinogen β chain precursor; FGG, fibrinogen γ chain precursor; SELENBP1, selenium binding protein 1; TXNIP, thioredoxin-interacting protein; PDZK1IP1, PDZK-interacting protein 1; DUSP6, dual specificity protein phosphatase 6.

Article Snippet: Then, the supernatants of each group of cells at 24, 48, 72 and 96 h were respectively collected to measure human complement factor C3 using the C3 ELISA kit (Cusabio, Wuhan, China) and C4b concentration using the C4b ELISA kit (Cusabio) according to the manufacturer's instructions (34).

Techniques: Microarray, Biomarker Discovery, Gene Expression, Real-time Polymerase Chain Reaction, Binding Assay

Figure 4. Effects of SLC34A2 on C3, C4b production and Pi absorption of A549 cells. (A) Effect of SLC34A2 on C3 production of A549 cells was detected by ELISA assay at consecutive time points. (B) Effect of SLC34A2 on the C4b production of A549 cells was detected by an ELISA assay at consecutive time points. (C) Effect of SLC34A2 on the absorption of Pi in A549 cells was assayed by the phosphomolybdic acid method at consecutive time points. The values are expressed as the mean ± standard error of the mean of three independent sets of data. **P<0.01. C3, complement C3 precursor; C4b, complement 4B prepropro tein; SLC34A2, solute carrier family 34 (sodium phosphate), member 2.

Journal: Molecular medicine reports

Article Title: Elevated expression of SLC34A2 inhibits the viability and invasion of A549 cells.

doi: 10.3892/mmr.2014.2376

Figure Lengend Snippet: Figure 4. Effects of SLC34A2 on C3, C4b production and Pi absorption of A549 cells. (A) Effect of SLC34A2 on C3 production of A549 cells was detected by ELISA assay at consecutive time points. (B) Effect of SLC34A2 on the C4b production of A549 cells was detected by an ELISA assay at consecutive time points. (C) Effect of SLC34A2 on the absorption of Pi in A549 cells was assayed by the phosphomolybdic acid method at consecutive time points. The values are expressed as the mean ± standard error of the mean of three independent sets of data. **P<0.01. C3, complement C3 precursor; C4b, complement 4B prepropro tein; SLC34A2, solute carrier family 34 (sodium phosphate), member 2.

Article Snippet: Then, the supernatants of each group of cells at 24, 48, 72 and 96 h were respectively collected to measure human complement factor C3 using the C3 ELISA kit (Cusabio, Wuhan, China) and C4b concentration using the C4b ELISA kit (Cusabio) according to the manufacturer's instructions (34).

Techniques: Enzyme-linked Immunosorbent Assay