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Roles of the lncRsn in mycelium penetration, infection structure, and <t>DON</t> production. ( a ) Mycelial penetration of wild-type PH-1 and ΔlncRsn strains on PSA medium after three days, with removal of aerial mycelium in cellophane after six days; ( b ) Infection structure formation of wild-type PH-1 and ΔlncRNA strains on wheat leaves after 24-48 h; ( c ) Analysis of DON toxin content in wheat kernels infected by different strains. ( d ) Analysis of DON toxin content of the strains in the culture supernatant incubated after seven days <t>of</t> <t>TBI,</t> p < 0.05, significant differences between the replicates are represented by letter a and b.
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Roles of the lncRsn in mycelium penetration, infection structure, and <t>DON</t> production. ( a ) Mycelial penetration of wild-type PH-1 and ΔlncRsn strains on PSA medium after three days, with removal of aerial mycelium in cellophane after six days; ( b ) Infection structure formation of wild-type PH-1 and ΔlncRNA strains on wheat leaves after 24-48 h; ( c ) Analysis of DON toxin content in wheat kernels infected by different strains. ( d ) Analysis of DON toxin content of the strains in the culture supernatant incubated after seven days <t>of</t> <t>TBI,</t> p < 0.05, significant differences between the replicates are represented by letter a and b.
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Roles of the lncRsn in mycelium penetration, infection structure, and <t>DON</t> production. ( a ) Mycelial penetration of wild-type PH-1 and ΔlncRsn strains on PSA medium after three days, with removal of aerial mycelium in cellophane after six days; ( b ) Infection structure formation of wild-type PH-1 and ΔlncRNA strains on wheat leaves after 24-48 h; ( c ) Analysis of DON toxin content in wheat kernels infected by different strains. ( d ) Analysis of DON toxin content of the strains in the culture supernatant incubated after seven days <t>of</t> <t>TBI,</t> p < 0.05, significant differences between the replicates are represented by letter a and b.
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Roles of the lncRsn in mycelium penetration, infection structure, and DON production. ( a ) Mycelial penetration of wild-type PH-1 and ΔlncRsn strains on PSA medium after three days, with removal of aerial mycelium in cellophane after six days; ( b ) Infection structure formation of wild-type PH-1 and ΔlncRNA strains on wheat leaves after 24-48 h; ( c ) Analysis of DON toxin content in wheat kernels infected by different strains. ( d ) Analysis of DON toxin content of the strains in the culture supernatant incubated after seven days of TBI, p < 0.05, significant differences between the replicates are represented by letter a and b.

Journal: Journal of Fungi

Article Title: An Antisense Long Non-Coding RNA, LncRsn, Is Involved in Sexual Reproduction and Full Virulence in Fusarium graminearum

doi: 10.3390/jof10100692

Figure Lengend Snippet: Roles of the lncRsn in mycelium penetration, infection structure, and DON production. ( a ) Mycelial penetration of wild-type PH-1 and ΔlncRsn strains on PSA medium after three days, with removal of aerial mycelium in cellophane after six days; ( b ) Infection structure formation of wild-type PH-1 and ΔlncRNA strains on wheat leaves after 24-48 h; ( c ) Analysis of DON toxin content in wheat kernels infected by different strains. ( d ) Analysis of DON toxin content of the strains in the culture supernatant incubated after seven days of TBI, p < 0.05, significant differences between the replicates are represented by letter a and b.

Article Snippet: A deoxynivalenol (DON) production assay was performed in TBI cultures using a competitive ELISA-based DON plate kit (Beacon Analytical Systems, Saco, ME, USA) as described [ ].

Techniques: Infection, Incubation