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Expected outcome of CellProfiler image analysis (A) Raw gray-scale image of a control well, recorded with the Opera Phenix high content screening system and merged using <t>CombineTIFF</t> software. Images are analyzed by open-source software CellProfiler. (B) Identification of the “Worm_4d” in a solvent control well (0.2% DMSO). Red number indicates worm area in pixel. (C) Identification of offspring (eggs + larvae) in a solvent control well (0.2% DMSO). Red numbers indicate the offspring count. (D) Example of a 4 μg/mL prochloraz treatment well containing a “Worm_4d_small”. Red number indicates worm area in pixel.
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Expected outcome of CellProfiler image analysis (A) Raw gray-scale image of a control well, recorded with the Opera Phenix high content screening system and merged using CombineTIFF software. Images are analyzed by open-source software CellProfiler. (B) Identification of the “Worm_4d” in a solvent control well (0.2% DMSO). Red number indicates worm area in pixel. (C) Identification of offspring (eggs + larvae) in a solvent control well (0.2% DMSO). Red numbers indicate the offspring count. (D) Example of a 4 μg/mL prochloraz treatment well containing a “Worm_4d_small”. Red number indicates worm area in pixel.

Journal: STAR Protocols

Article Title: A quantitative medium-throughput assay to measure Caenorhabditis elegans development and reproduction

doi: 10.1016/j.xpro.2020.100224

Figure Lengend Snippet: Expected outcome of CellProfiler image analysis (A) Raw gray-scale image of a control well, recorded with the Opera Phenix high content screening system and merged using CombineTIFF software. Images are analyzed by open-source software CellProfiler. (B) Identification of the “Worm_4d” in a solvent control well (0.2% DMSO). Red number indicates worm area in pixel. (C) Identification of offspring (eggs + larvae) in a solvent control well (0.2% DMSO). Red numbers indicate the offspring count. (D) Example of a 4 μg/mL prochloraz treatment well containing a “Worm_4d_small”. Red number indicates worm area in pixel.

Article Snippet: As a merging tool, we use “CombineTIFF”, a self-programmed MATLAB-based program specialized for our needs when processing images generated with the Opera Phenix High Content Screening System.

Techniques: Control, High Content Screening, Software, Solvent

Journal: STAR Protocols

Article Title: A quantitative medium-throughput assay to measure Caenorhabditis elegans development and reproduction

doi: 10.1016/j.xpro.2020.100224

Figure Lengend Snippet:

Article Snippet: As a merging tool, we use “CombineTIFF”, a self-programmed MATLAB-based program specialized for our needs when processing images generated with the Opera Phenix High Content Screening System.

Techniques: Virus, Recombinant, Software, Fluorescence, Microscopy, High Content Screening