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CD166 promotes PDAC tumor stiffness through LOXL2. (A, C) Results of elastography to test the tumor stiffness in the in situ PDAC tumor model. (B, D) Results of Masson's trichrome staining to detect the degree of fibrosis of the in situ tumor model of PDAC. (E-F) Results of the matrix cross-linking experiment of BxPC-3 PDAC cells after knocking down CD166 (E) and LOXL2 (F). (G-H) An electronic universal testing machine is used to measure the matrix stiffness <t>of</t> <t>collagen</t> fibers after 3D culture. (I-J) Morphological changes in the <t>type</t> <t>I</t> collagen fiber matrix observed under an electron microscope after knocking down CD166 (I) and LOXL2 (J). (K-L) Tumor size in the in situ tumor model mice. (M-N) Tumor weight in the in situ tumor model mice.
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CD166 promotes PDAC tumor stiffness through LOXL2. (A, C) Results of elastography to test the tumor stiffness in the in situ PDAC tumor model. (B, D) Results of Masson's trichrome staining to detect the degree of fibrosis of the in situ tumor model of PDAC. (E-F) Results of the matrix cross-linking experiment of BxPC-3 PDAC cells after knocking down CD166 (E) and LOXL2 (F). (G-H) An electronic universal testing machine is used to measure the matrix stiffness <t>of</t> <t>collagen</t> fibers after 3D culture. (I-J) Morphological changes in the <t>type</t> <t>I</t> collagen fiber matrix observed under an electron microscope after knocking down CD166 (I) and LOXL2 (J). (K-L) Tumor size in the in situ tumor model mice. (M-N) Tumor weight in the in situ tumor model mice.
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Application of <t>PCFS</t> scaffolds loaded with CBD-NT-3 to boost functional neuronal growth and axonal regeneration for the treatment of SCI. (A) Schematic representation of SCI repair using PAO to create a biomimetic collagen scaffold with unique physical properties. (B, C) Immunofluorescence staining maps of Tuj-1 + and Map-2 + in the injured sites of the individual treatment groups. Scale bars: 1 mm (left) and 50 μm (right and below). (D) Quantitative analysis of the area of Tuj-1 + and Map-2 + cells at the injury site in the different treatment groups. * P < 0.05, ** P < 0.01, *** P < 0.001. Reprinted with permission from Zhao et al. (2024). Copyright 2024 Wiley‐VCH GmbH. CBD-NT-3: Collagen-binding domain-neurotrophin-3; DAPI: 4′,6-diamidino-2-phenylindole; EDC: N-ethyl-N′-(3-(dimethylamino)propyl)carbodiimide; LOCS: linear ordered collagen scaffolds; Map-2: microtubule-associated protein-2; NHS: N-hydroxysuccinimide; NSC: neural stem cell; PAO: plasma amine oxidase; PCFS: porous collagen fibers; SCI: spinal cord injury.
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Application of <t>PCFS</t> scaffolds loaded with CBD-NT-3 to boost functional neuronal growth and axonal regeneration for the treatment of SCI. (A) Schematic representation of SCI repair using PAO to create a biomimetic collagen scaffold with unique physical properties. (B, C) Immunofluorescence staining maps of Tuj-1 + and Map-2 + in the injured sites of the individual treatment groups. Scale bars: 1 mm (left) and 50 μm (right and below). (D) Quantitative analysis of the area of Tuj-1 + and Map-2 + cells at the injury site in the different treatment groups. * P < 0.05, ** P < 0.01, *** P < 0.001. Reprinted with permission from Zhao et al. (2024). Copyright 2024 Wiley‐VCH GmbH. CBD-NT-3: Collagen-binding domain-neurotrophin-3; DAPI: 4′,6-diamidino-2-phenylindole; EDC: N-ethyl-N′-(3-(dimethylamino)propyl)carbodiimide; LOCS: linear ordered collagen scaffolds; Map-2: microtubule-associated protein-2; NHS: N-hydroxysuccinimide; NSC: neural stem cell; PAO: plasma amine oxidase; PCFS: porous collagen fibers; SCI: spinal cord injury.
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Image Search Results


CD166 promotes PDAC tumor stiffness through LOXL2. (A, C) Results of elastography to test the tumor stiffness in the in situ PDAC tumor model. (B, D) Results of Masson's trichrome staining to detect the degree of fibrosis of the in situ tumor model of PDAC. (E-F) Results of the matrix cross-linking experiment of BxPC-3 PDAC cells after knocking down CD166 (E) and LOXL2 (F). (G-H) An electronic universal testing machine is used to measure the matrix stiffness of collagen fibers after 3D culture. (I-J) Morphological changes in the type I collagen fiber matrix observed under an electron microscope after knocking down CD166 (I) and LOXL2 (J). (K-L) Tumor size in the in situ tumor model mice. (M-N) Tumor weight in the in situ tumor model mice.

Journal: International Journal of Biological Sciences

Article Title: Interaction between m6A and YAP1 mechanotransduction pathways is essential for mechanical memory and matrix remodeling in pancreatic cancer

doi: 10.7150/ijbs.125330

Figure Lengend Snippet: CD166 promotes PDAC tumor stiffness through LOXL2. (A, C) Results of elastography to test the tumor stiffness in the in situ PDAC tumor model. (B, D) Results of Masson's trichrome staining to detect the degree of fibrosis of the in situ tumor model of PDAC. (E-F) Results of the matrix cross-linking experiment of BxPC-3 PDAC cells after knocking down CD166 (E) and LOXL2 (F). (G-H) An electronic universal testing machine is used to measure the matrix stiffness of collagen fibers after 3D culture. (I-J) Morphological changes in the type I collagen fiber matrix observed under an electron microscope after knocking down CD166 (I) and LOXL2 (J). (K-L) Tumor size in the in situ tumor model mice. (M-N) Tumor weight in the in situ tumor model mice.

Article Snippet: Ten million pancreatic cancer cells from culture dishes were digested and resuspended in 500 μL type I collagen fibers (Thermo Fisher, USA).

Techniques: In Situ, Staining, Microscopy

Application of PCFS scaffolds loaded with CBD-NT-3 to boost functional neuronal growth and axonal regeneration for the treatment of SCI. (A) Schematic representation of SCI repair using PAO to create a biomimetic collagen scaffold with unique physical properties. (B, C) Immunofluorescence staining maps of Tuj-1 + and Map-2 + in the injured sites of the individual treatment groups. Scale bars: 1 mm (left) and 50 μm (right and below). (D) Quantitative analysis of the area of Tuj-1 + and Map-2 + cells at the injury site in the different treatment groups. * P < 0.05, ** P < 0.01, *** P < 0.001. Reprinted with permission from Zhao et al. (2024). Copyright 2024 Wiley‐VCH GmbH. CBD-NT-3: Collagen-binding domain-neurotrophin-3; DAPI: 4′,6-diamidino-2-phenylindole; EDC: N-ethyl-N′-(3-(dimethylamino)propyl)carbodiimide; LOCS: linear ordered collagen scaffolds; Map-2: microtubule-associated protein-2; NHS: N-hydroxysuccinimide; NSC: neural stem cell; PAO: plasma amine oxidase; PCFS: porous collagen fibers; SCI: spinal cord injury.

Journal: Neural Regeneration Research

Article Title: Biomaterial-based strategies: a new era in spinal cord injury treatment

doi: 10.4103/NRR.NRR-D-24-00844

Figure Lengend Snippet: Application of PCFS scaffolds loaded with CBD-NT-3 to boost functional neuronal growth and axonal regeneration for the treatment of SCI. (A) Schematic representation of SCI repair using PAO to create a biomimetic collagen scaffold with unique physical properties. (B, C) Immunofluorescence staining maps of Tuj-1 + and Map-2 + in the injured sites of the individual treatment groups. Scale bars: 1 mm (left) and 50 μm (right and below). (D) Quantitative analysis of the area of Tuj-1 + and Map-2 + cells at the injury site in the different treatment groups. * P < 0.05, ** P < 0.01, *** P < 0.001. Reprinted with permission from Zhao et al. (2024). Copyright 2024 Wiley‐VCH GmbH. CBD-NT-3: Collagen-binding domain-neurotrophin-3; DAPI: 4′,6-diamidino-2-phenylindole; EDC: N-ethyl-N′-(3-(dimethylamino)propyl)carbodiimide; LOCS: linear ordered collagen scaffolds; Map-2: microtubule-associated protein-2; NHS: N-hydroxysuccinimide; NSC: neural stem cell; PAO: plasma amine oxidase; PCFS: porous collagen fibers; SCI: spinal cord injury.

Article Snippet: Zhao et al. (2024) developed a dual-network porous collagen fiber (PCFS) scaffold for neurogenesis using a combination of biomimetic plasma ammonia oxidase catalysis and conventional amide cross-linking.

Techniques: Functional Assay, Immunofluorescence, Staining, Binding Assay, Clinical Proteomics