Journal: IBRO Neuroscience Reports

Article Title: Ondansetron reduces seizures and improves cognitive impairment in a rat model of temporal lobe epilepsy

doi: 10.1016/j.ibneur.2025.12.009

Figure Lengend Snippet: Ondansetron treatment inhibits synaptic reorganization in TLE rats. A: Golgi staining analysis of dendritic spines in TLE rats; B: Dendritic spines densities were analysed by quantitative; C: Double immunofluorescence staining on primary hippocampal neurons using antibodies against synaptophysin (SYP, green) and PSD95 (red); D: Phalloidin staining analysis of hippocampal neuron; E: Western blot analyzed the proteins of F-actin and cofilin-1 expression in the hippocampus; F: Quantitative analysis F-actin expression; G: Quantitative analysis cofilin-1 expression; * P < 0.05, control vs. epilepsy; # P < 0.05, Ondansetron + epilepsy vs. epilepsy; Mean ± SEM, n = 4.

Article Snippet: Membranes were blocked with 5 % non-fat dry milk in Tris-buffered saline containing 0.1 % Tween-20 (TBST) for 1 h at room temperature, then incubated overnight at 4°C with the following primary antibodies: Cofilin-1 (1:500; Cell Signaling Technology) and F-actin (1:1000; Cell Signaling Technology).

Techniques: Staining, Double Immunofluorescence Staining, Western Blot, Expressing, Control