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codon optimized cas9  (Addgene inc)


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    Addgene inc codon optimized cas9
    Codon Optimized Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/codon+optimized+cas9/LentiV_Cas9_puro+(Plasmid+%23108100)/bio_rxiv__64898__2026__01__21__700714-205-16-22
    Average 96 stars, based on 89 article reviews
    codon optimized cas9 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Construct:

    Article Title: BRD8 Disruption Unlocks Retrotransposon-Driven Tumor-Intrinsic Immunogenicity via Redistribution of Histone Acetylation in Liver Cancer
    Article Snippet: .. All Cas9-expressing cell lines used in this study were constructed through lentiviral transduction with a 5′-Flag-tagged human-codon optimized Cas9 by plasmid LentiV_Cas9_puro (Addgene, 108100). .. Individual sgRNAs were cloned into lentiviral expression vectors with an optimized sgRNA scaffold backbone plasmid LRG2.1 (Addgene, 108098) or LRB2.1.

    Transduction:

    Article Title: BRD8 Disruption Unlocks Retrotransposon-Driven Tumor-Intrinsic Immunogenicity via Redistribution of Histone Acetylation in Liver Cancer
    Article Snippet: .. All Cas9-expressing cell lines used in this study were constructed through lentiviral transduction with a 5′-Flag-tagged human-codon optimized Cas9 by plasmid LentiV_Cas9_puro (Addgene, 108100). .. Individual sgRNAs were cloned into lentiviral expression vectors with an optimized sgRNA scaffold backbone plasmid LRG2.1 (Addgene, 108098) or LRB2.1.

    Plasmid Preparation:

    Article Title: BRD8 Disruption Unlocks Retrotransposon-Driven Tumor-Intrinsic Immunogenicity via Redistribution of Histone Acetylation in Liver Cancer
    Article Snippet: .. All Cas9-expressing cell lines used in this study were constructed through lentiviral transduction with a 5′-Flag-tagged human-codon optimized Cas9 by plasmid LentiV_Cas9_puro (Addgene, 108100). .. Individual sgRNAs were cloned into lentiviral expression vectors with an optimized sgRNA scaffold backbone plasmid LRG2.1 (Addgene, 108098) or LRB2.1.

    Article Title: Opportunities for CRISPR/Cas9 Gene Editing in Retinal Regeneration Research
    Article Snippet: .. Codon-optimized Cas9 (Addgene: 64237) , Plasmid , Yin et al., . .. Vector system for tissue-specific gene disruption (Addgene: 63154, 63155, 63156, 63157) , Plasmids , Ablain et al., .



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    (A) Schematic workflow of the pooled <t>CRISPR/Cas9</t> gene knockout screen. Cas9-expressing chromaffin cells were generated via lentiviral transduction and blasticidin selection. Cells were then transduced with a genome-wide sgRNA library, selected with puromycin, and cultured for 10 population doublings before genomic DNA extraction. sgRNA abundance was quantified at initial and final times by deep sequencing, and data were analyzed using the MAGeCK pipeline. (B) Ranked Robust Rank Aggregation (RRA) scores of the most significantly depleted genes from the screen. (C) Ranked RRA scores of the most significantly enriched genes from the screen. (D) Volcano plot of CRISPR screen results showing significantly depleted genes (blue) versus non-significant genes (gray). The x-axis represents log₂(fold change) (Log₂FC) in sgRNA abundance, and the y-axis shows –log₁₀ P values from MAGeCK analysis. (E) Volcano plot showing significantly enriched genes (red) versus non-significant genes (gray).
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    Image Search Results


    (A) Schematic workflow of the pooled CRISPR/Cas9 gene knockout screen. Cas9-expressing chromaffin cells were generated via lentiviral transduction and blasticidin selection. Cells were then transduced with a genome-wide sgRNA library, selected with puromycin, and cultured for 10 population doublings before genomic DNA extraction. sgRNA abundance was quantified at initial and final times by deep sequencing, and data were analyzed using the MAGeCK pipeline. (B) Ranked Robust Rank Aggregation (RRA) scores of the most significantly depleted genes from the screen. (C) Ranked RRA scores of the most significantly enriched genes from the screen. (D) Volcano plot of CRISPR screen results showing significantly depleted genes (blue) versus non-significant genes (gray). The x-axis represents log₂(fold change) (Log₂FC) in sgRNA abundance, and the y-axis shows –log₁₀ P values from MAGeCK analysis. (E) Volcano plot showing significantly enriched genes (red) versus non-significant genes (gray).

    Journal: bioRxiv

    Article Title: Unbiased CRISPR Synthetic Lethal Screening for Genetic Vulnerabilities in Succinate Dehydrogenase (SDH)-loss Model of Paraganglioma

    doi: 10.1101/2025.08.26.672429

    Figure Lengend Snippet: (A) Schematic workflow of the pooled CRISPR/Cas9 gene knockout screen. Cas9-expressing chromaffin cells were generated via lentiviral transduction and blasticidin selection. Cells were then transduced with a genome-wide sgRNA library, selected with puromycin, and cultured for 10 population doublings before genomic DNA extraction. sgRNA abundance was quantified at initial and final times by deep sequencing, and data were analyzed using the MAGeCK pipeline. (B) Ranked Robust Rank Aggregation (RRA) scores of the most significantly depleted genes from the screen. (C) Ranked RRA scores of the most significantly enriched genes from the screen. (D) Volcano plot of CRISPR screen results showing significantly depleted genes (blue) versus non-significant genes (gray). The x-axis represents log₂(fold change) (Log₂FC) in sgRNA abundance, and the y-axis shows –log₁₀ P values from MAGeCK analysis. (E) Volcano plot showing significantly enriched genes (red) versus non-significant genes (gray).

    Article Snippet: Parental imCC lines were made to express Cas9 by transduction with 3 rd generation lentiviral particles carrying codon-optimized S. pyogenes Cas9 protein and blasticidin resistance driven from the EFS promoter (Addgene # 52962-LV).

    Techniques: CRISPR, Gene Knockout, Expressing, Generated, Transduction, Selection, Genome Wide, Cell Culture, DNA Extraction, Sequencing