Journal: Genes, Brain, and Behavior

Article Title: Repeated Stress Escalates Aggression and Activity in Fronto‐Limbic Regions in Cntnap2 − / − Mice

doi: 10.1111/gbb.70048

Figure Lengend Snippet: Increased aggressive behavior in Cntnap2 −/− mice following repeated restraint stress. (A) Experimental timeline of 4‐day SIA paradigm. (B–D) Quantification of duration of aggressive behavior in control (black) and Cntnap2 −/− (red) mice at baseline (BL) and posttest (PT). (E–G) Quantification of the total number of aggressive events in control (black) and Cntnap2 −/− (red) mice at BL and PT. (H–J) Quantification of latency to aggression in control (black) and Cntnap2 −/− (red) mice at baseline (BL) and posttest (PT). Data shown are mean ± s.e.m. with individual and average data points plotted. Repeated measures two‐way ANOVA with uncorrected Fisher's LSD multiple comparisons post hoc tests; ** p < 0.01, **** p < 0.0001.

Article Snippet: The following transgenic lines (all on C57BL/6J backgrounds) were used at 6–22 weeks of age: B6.129(Cg)‐ Cntnap2 tm1Pele /J (Jackson Laboratory, stock number 028635), a knockout line for the Cntnap2 gene [ ].

Techniques: Control

Journal: Genes, Brain, and Behavior

Article Title: Repeated Stress Escalates Aggression and Activity in Fronto‐Limbic Regions in Cntnap2 − / − Mice

doi: 10.1111/gbb.70048

Figure Lengend Snippet: Region specific activation patterns and TRAP cell count comparison for the LS, LHb, and LH. (A) Visual representation of TRAP+ cells in the LS (top), LHb and LH (bottom) in control (left) and Cntnap2 −/− (right) mice. (B–D) Quantification of TRAP+ cells within the LS (B), LHb (C), and LH (D) in control (black) and Cntnap2 −/− (red) mice. Blue cells are DAPI stained and red cells are TdTomato (TRAP+) neurons. Data shown are mean ± s.e.m. with individual data points plotted. Two‐tailed unpaired t ‐test; *** p < 0.001.

Article Snippet: The following transgenic lines (all on C57BL/6J backgrounds) were used at 6–22 weeks of age: B6.129(Cg)‐ Cntnap2 tm1Pele /J (Jackson Laboratory, stock number 028635), a knockout line for the Cntnap2 gene [ ].

Techniques: Activation Assay, Cell Characterization, Comparison, Control, Staining, Two Tailed Test

Journal: Genes, Brain, and Behavior

Article Title: Repeated Stress Escalates Aggression and Activity in Fronto‐Limbic Regions in Cntnap2 − / − Mice

doi: 10.1111/gbb.70048

Figure Lengend Snippet: Region specific activation patterns and TRAP cell count comparison for the NAc and mPFC. (A) Visual representation of TRAP+ cells in the NAc in control (left) and Cntnap2 −/− (right) mice. (B–D) Quantification of TRAP+ cells within the total NAc (B), NAc core (C), and NAc shell (D) in control (black) and Cntnap2 −/− (red) mice. (E) Visual representation of TRAP+ cells in the mPFC in control (left) and Cntnap2 −/− (right) mice. (F–H) Quantification of TRAP+ cells within the total mPFC (F), PL (G), and IL (H) in control (black) and Cntnap2 −/− (red) mice. Blue cells are DAPI stained and red cells are TdTomato (TRAP+) neurons. Data shown are mean ± s.e.m. with individual and average data points plotted. Two‐tailed unpaired t‐test; * p < 0.05.

Article Snippet: The following transgenic lines (all on C57BL/6J backgrounds) were used at 6–22 weeks of age: B6.129(Cg)‐ Cntnap2 tm1Pele /J (Jackson Laboratory, stock number 028635), a knockout line for the Cntnap2 gene [ ].

Techniques: Activation Assay, Cell Characterization, Comparison, Control, Staining, Two Tailed Test

Journal: Genes, Brain, and Behavior

Article Title: Repeated Stress Escalates Aggression and Activity in Fronto‐Limbic Regions in Cntnap2 − / − Mice

doi: 10.1111/gbb.70048

Figure Lengend Snippet: Positive correlation between the time aggressive and TRAP+ cell counts in the LS, LHb, and IL. (A–C) Significant positive correlations between the aggressive time and TRAP+ cells in the LS (A) and LHb (B), but not the LH (C) in control (black) and Cntnap2 −/− (red) mice. (D–F) No significant correlation between aggressive time and TRAP+ cells in NAc total (D), or its subregions, NAc core (E) and shell (F) in control (black) and Cntnap2 −/− (red) mice. (G–I) No significant correlations in mPFC total (G) or PL (H), but the IL showed significant positive correlations between time aggressive and TRAP+ cells (I) in control (black) and Cntnap2 −/− (red) mice. Simple linear regression.

Article Snippet: The following transgenic lines (all on C57BL/6J backgrounds) were used at 6–22 weeks of age: B6.129(Cg)‐ Cntnap2 tm1Pele /J (Jackson Laboratory, stock number 028635), a knockout line for the Cntnap2 gene [ ].

Techniques: Control