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A. Vimentin SMA and F-actin staining of M. Sch and P. Meso primary fibroblasts. Green: Vimentin; Yellow: SMA; Magenta: F-actin; Blue: DAPI. B. Vimentin and SMA staining of M. Sch fibroblasts -TERT-GFP and P. Meso fibroblasts <t>-SV40-GFP/</t> hTERT-GFP. Green: GFP; Yellow: SMA; Magenta: Vimentin; Blue: DAPI.
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A. Vimentin SMA and F-actin staining of M. Sch and P. Meso primary fibroblasts. Green: Vimentin; Yellow: SMA; Magenta: F-actin; Blue: DAPI. B. Vimentin and SMA staining of M. Sch fibroblasts -TERT-GFP and P. Meso fibroblasts -SV40-GFP/ hTERT-GFP. Green: GFP; Yellow: SMA; Magenta: Vimentin; Blue: DAPI.

Journal: bioRxiv

Article Title: Establishing Primary and Stable Cell Lines from Frozen Wing Biopsies for Cellular, Physiological, and Genetic Studies in Bats

doi: 10.1101/2024.03.22.586286

Figure Lengend Snippet: A. Vimentin SMA and F-actin staining of M. Sch and P. Meso primary fibroblasts. Green: Vimentin; Yellow: SMA; Magenta: F-actin; Blue: DAPI. B. Vimentin and SMA staining of M. Sch fibroblasts -TERT-GFP and P. Meso fibroblasts -SV40-GFP/ hTERT-GFP. Green: GFP; Yellow: SMA; Magenta: Vimentin; Blue: DAPI.

Article Snippet: Additional Materials (other materials are included in basic protocol 1 and 2) hTERT (CMV, GFP-Puro) lentivirus in PBS (GenTarget Inc, Cat#: LVP1130-GP-PBS) SV40 large T-antigen (CMV, GFP-Puro) lentivirus in PBS (GenTarget Inc, Cat#: LVP016-GP)

Techniques: Staining