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Results of DNA barcoding and susceptibility tests Species were confirmed by DNA barcoding using nucleotide sequences of internal transcribed spacer (ITS: ITS1/ITS4 primer [ <xref ref-type= 5 ]) small subunit region (SSU: NS1/NS2 primer [ 5 ]) and large subunit region (LSU: NL-1/NL-4 primer [ 6 ]) of the ribosomal DNA gene. The purified PCR products were then outsourced for Sanger sequencing service (First BASE Laboratories, Singapore Science Park II, Singapore). The sequencing results were subjected to BLASTN (National Center for Biotechnology Information Internet homepage) search against the GenBank database for homology identities. Antifungal susceptibility patterns of the mold against common antifungal medications were assessed by the broth dilution technique (according to the Clinical and Laboratory Standards Institute (CLSI) document M38-A2 protocol) with reference to Aspergillus spp . AMB, amphotericin B; CAS, caspofungin; FLC, fluconazole; ISZ, isavuconazole; ITZ, itaconazole; PSZ, Posaconazole; TBF=terbinafine; VCZ, voriconazole; (R), resistance; (S), susceptible; (N/A), non-available" width="250" height="auto" />
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Results of DNA barcoding and susceptibility tests Species were confirmed by DNA barcoding using nucleotide sequences of internal transcribed spacer (ITS: ITS1/ITS4 primer [ <xref ref-type= 5 ]) small subunit region (SSU: NS1/NS2 primer [ 5 ]) and large subunit region (LSU: NL-1/NL-4 primer [ 6 ]) of the ribosomal DNA gene. The purified PCR products were then outsourced for Sanger sequencing service (First BASE Laboratories, Singapore Science Park II, Singapore). The sequencing results were subjected to BLASTN (National Center for Biotechnology Information Internet homepage) search against the GenBank database for homology identities. Antifungal susceptibility patterns of the mold against common antifungal medications were assessed by the broth dilution technique (according to the Clinical and Laboratory Standards Institute (CLSI) document M38-A2 protocol) with reference to Aspergillus spp . AMB, amphotericin B; CAS, caspofungin; FLC, fluconazole; ISZ, isavuconazole; ITZ, itaconazole; PSZ, Posaconazole; TBF=terbinafine; VCZ, voriconazole; (R), resistance; (S), susceptible; (N/A), non-available" width="250" height="auto" />
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Results of DNA barcoding and susceptibility tests Species were confirmed by DNA barcoding using nucleotide sequences of internal transcribed spacer (ITS: ITS1/ITS4 primer [ <xref ref-type= 5 ]) small subunit region (SSU: NS1/NS2 primer [ 5 ]) and large subunit region (LSU: NL-1/NL-4 primer [ 6 ]) of the ribosomal DNA gene. The purified PCR products were then outsourced for Sanger sequencing service (First BASE Laboratories, Singapore Science Park II, Singapore). The sequencing results were subjected to BLASTN (National Center for Biotechnology Information Internet homepage) search against the GenBank database for homology identities. Antifungal susceptibility patterns of the mold against common antifungal medications were assessed by the broth dilution technique (according to the Clinical and Laboratory Standards Institute (CLSI) document M38-A2 protocol) with reference to Aspergillus spp . AMB, amphotericin B; CAS, caspofungin; FLC, fluconazole; ISZ, isavuconazole; ITZ, itaconazole; PSZ, Posaconazole; TBF=terbinafine; VCZ, voriconazole; (R), resistance; (S), susceptible; (N/A), non-available" width="250" height="auto" />
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Results of DNA barcoding and susceptibility tests Species were confirmed by DNA barcoding using nucleotide sequences of internal transcribed spacer (ITS: ITS1/ITS4 primer [ <xref ref-type= 5 ]) small subunit region (SSU: NS1/NS2 primer [ 5 ]) and large subunit region (LSU: NL-1/NL-4 primer [ 6 ]) of the ribosomal DNA gene. The purified PCR products were then outsourced for Sanger sequencing service (First BASE Laboratories, Singapore Science Park II, Singapore). The sequencing results were subjected to BLASTN (National Center for Biotechnology Information Internet homepage) search against the GenBank database for homology identities. Antifungal susceptibility patterns of the mold against common antifungal medications were assessed by the broth dilution technique (according to the Clinical and Laboratory Standards Institute (CLSI) document M38-A2 protocol) with reference to Aspergillus spp . AMB, amphotericin B; CAS, caspofungin; FLC, fluconazole; ISZ, isavuconazole; ITZ, itaconazole; PSZ, Posaconazole; TBF=terbinafine; VCZ, voriconazole; (R), resistance; (S), susceptible; (N/A), non-available" width="250" height="auto" />
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Results of DNA barcoding and susceptibility tests Species were confirmed by DNA barcoding using nucleotide sequences of internal transcribed spacer (ITS: ITS1/ITS4 primer [ <xref ref-type= 5 ]) small subunit region (SSU: NS1/NS2 primer [ 5 ]) and large subunit region (LSU: NL-1/NL-4 primer [ 6 ]) of the ribosomal DNA gene. The purified PCR products were then outsourced for Sanger sequencing service (First BASE Laboratories, Singapore Science Park II, Singapore). The sequencing results were subjected to BLASTN (National Center for Biotechnology Information Internet homepage) search against the GenBank database for homology identities. Antifungal susceptibility patterns of the mold against common antifungal medications were assessed by the broth dilution technique (according to the Clinical and Laboratory Standards Institute (CLSI) document M38-A2 protocol) with reference to Aspergillus spp . AMB, amphotericin B; CAS, caspofungin; FLC, fluconazole; ISZ, isavuconazole; ITZ, itaconazole; PSZ, Posaconazole; TBF=terbinafine; VCZ, voriconazole; (R), resistance; (S), susceptible; (N/A), non-available" width="100%" height="100%">

Journal: Cureus

Article Title: Peritonitis Caused by Various Species of Diaporthe in Peritoneal Dialysis Patients: A Plant Pathogen to Human Infection

doi: 10.7759/cureus.57016

Figure Lengend Snippet: Results of DNA barcoding and susceptibility tests Species were confirmed by DNA barcoding using nucleotide sequences of internal transcribed spacer (ITS: ITS1/ITS4 primer [ 5 ]) small subunit region (SSU: NS1/NS2 primer [ 5 ]) and large subunit region (LSU: NL-1/NL-4 primer [ 6 ]) of the ribosomal DNA gene. The purified PCR products were then outsourced for Sanger sequencing service (First BASE Laboratories, Singapore Science Park II, Singapore). The sequencing results were subjected to BLASTN (National Center for Biotechnology Information Internet homepage) search against the GenBank database for homology identities. Antifungal susceptibility patterns of the mold against common antifungal medications were assessed by the broth dilution technique (according to the Clinical and Laboratory Standards Institute (CLSI) document M38-A2 protocol) with reference to Aspergillus spp . AMB, amphotericin B; CAS, caspofungin; FLC, fluconazole; ISZ, isavuconazole; ITZ, itaconazole; PSZ, Posaconazole; TBF=terbinafine; VCZ, voriconazole; (R), resistance; (S), susceptible; (N/A), non-available

Article Snippet: Minimum inhibitory concentrations (MIC) values for various antifungal agents were determined using the modified Clinical and Laboratory Standards Institute (CLSI) M38-A broth microdilution method [ ] with reference to Aspergillus spp [ ].

Techniques: Purification, Sequencing, Medications, Activity Assay, Infection