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Characterization of microspheres. (a) Scanning electronic microscopic (SEM) observations shows the morphology and surface structure of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h. (b) Fourier transform infrared spectroscopy (FTIR) analysis showing the different second structure of SF (β-sheet, random coil/helix and β-turn) <t>content</t> in different recrystallization time. (c) Matched curve of β-sheet fraction with assembly time. Illustrations showed the morphology of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h, as well as the Fourier transform infrared spectroscopy (FTIR) analysis showing the change of β-sheet content in different recrystallization time. (d, e) Cumulative release curve of MAP and KGN from PSF and KSF with different β-sheet fraction respectively. (f) The contact angle with water of SF with different β-sheet fraction showing the wetting property. (g) The degradation of SF with different β-sheet fraction. (h) Comparison of release kinetics for drug releasing strategies between our approach (red line) and previous studies reported . The illustration showed the comparison of release kinetics for MSC recruiting strategies between our approach (gray area) and previous studies (purple points). (i) In vivo retention of PSF microspheres injected in the joint cavity visualized by in vivo <t>imaging</t> <t>system</t> (IVIS). (j) Fluorescent intensity (Taking the base-10 logarithm) at different timepoint after injection. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
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Characterization of microspheres. (a) Scanning electronic microscopic (SEM) observations shows the morphology and surface structure of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h. (b) Fourier transform infrared spectroscopy (FTIR) analysis showing the different second structure of SF (β-sheet, random coil/helix and β-turn) <t>content</t> in different recrystallization time. (c) Matched curve of β-sheet fraction with assembly time. Illustrations showed the morphology of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h, as well as the Fourier transform infrared spectroscopy (FTIR) analysis showing the change of β-sheet content in different recrystallization time. (d, e) Cumulative release curve of MAP and KGN from PSF and KSF with different β-sheet fraction respectively. (f) The contact angle with water of SF with different β-sheet fraction showing the wetting property. (g) The degradation of SF with different β-sheet fraction. (h) Comparison of release kinetics for drug releasing strategies between our approach (red line) and previous studies reported . The illustration showed the comparison of release kinetics for MSC recruiting strategies between our approach (gray area) and previous studies (purple points). (i) In vivo retention of PSF microspheres injected in the joint cavity visualized by in vivo <t>imaging</t> <t>system</t> (IVIS). (j) Fluorescent intensity (Taking the base-10 logarithm) at different timepoint after injection. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
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Characterization of microspheres. (a) Scanning electronic microscopic (SEM) observations shows the morphology and surface structure of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h. (b) Fourier transform infrared spectroscopy (FTIR) analysis showing the different second structure of SF (β-sheet, random coil/helix and β-turn) <t>content</t> in different recrystallization time. (c) Matched curve of β-sheet fraction with assembly time. Illustrations showed the morphology of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h, as well as the Fourier transform infrared spectroscopy (FTIR) analysis showing the change of β-sheet content in different recrystallization time. (d, e) Cumulative release curve of MAP and KGN from PSF and KSF with different β-sheet fraction respectively. (f) The contact angle with water of SF with different β-sheet fraction showing the wetting property. (g) The degradation of SF with different β-sheet fraction. (h) Comparison of release kinetics for drug releasing strategies between our approach (red line) and previous studies reported . The illustration showed the comparison of release kinetics for MSC recruiting strategies between our approach (gray area) and previous studies (purple points). (i) In vivo retention of PSF microspheres injected in the joint cavity visualized by in vivo <t>imaging</t> <t>system</t> (IVIS). (j) Fluorescent intensity (Taking the base-10 logarithm) at different timepoint after injection. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
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Characterization of microspheres. (a) Scanning electronic microscopic (SEM) observations shows the morphology and surface structure of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h. (b) Fourier transform infrared spectroscopy (FTIR) analysis showing the different second structure of SF (β-sheet, random coil/helix and β-turn) <t>content</t> in different recrystallization time. (c) Matched curve of β-sheet fraction with assembly time. Illustrations showed the morphology of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h, as well as the Fourier transform infrared spectroscopy (FTIR) analysis showing the change of β-sheet content in different recrystallization time. (d, e) Cumulative release curve of MAP and KGN from PSF and KSF with different β-sheet fraction respectively. (f) The contact angle with water of SF with different β-sheet fraction showing the wetting property. (g) The degradation of SF with different β-sheet fraction. (h) Comparison of release kinetics for drug releasing strategies between our approach (red line) and previous studies reported . The illustration showed the comparison of release kinetics for MSC recruiting strategies between our approach (gray area) and previous studies (purple points). (i) In vivo retention of PSF microspheres injected in the joint cavity visualized by in vivo <t>imaging</t> <t>system</t> (IVIS). (j) Fluorescent intensity (Taking the base-10 logarithm) at different timepoint after injection. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
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Characterization of microspheres. (a) Scanning electronic microscopic (SEM) observations shows the morphology and surface structure of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h. (b) Fourier transform infrared spectroscopy (FTIR) analysis showing the different second structure of SF (β-sheet, random coil/helix and β-turn) <t>content</t> in different recrystallization time. (c) Matched curve of β-sheet fraction with assembly time. Illustrations showed the morphology of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h, as well as the Fourier transform infrared spectroscopy (FTIR) analysis showing the change of β-sheet content in different recrystallization time. (d, e) Cumulative release curve of MAP and KGN from PSF and KSF with different β-sheet fraction respectively. (f) The contact angle with water of SF with different β-sheet fraction showing the wetting property. (g) The degradation of SF with different β-sheet fraction. (h) Comparison of release kinetics for drug releasing strategies between our approach (red line) and previous studies reported . The illustration showed the comparison of release kinetics for MSC recruiting strategies between our approach (gray area) and previous studies (purple points). (i) In vivo retention of PSF microspheres injected in the joint cavity visualized by in vivo <t>imaging</t> <t>system</t> (IVIS). (j) Fluorescent intensity (Taking the base-10 logarithm) at different timepoint after injection. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
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Characterization of microspheres. (a) Scanning electronic microscopic (SEM) observations shows the morphology and surface structure of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h. (b) Fourier transform infrared spectroscopy (FTIR) analysis showing the different second structure of SF (β-sheet, random coil/helix and β-turn) <t>content</t> in different recrystallization time. (c) Matched curve of β-sheet fraction with assembly time. Illustrations showed the morphology of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h, as well as the Fourier transform infrared spectroscopy (FTIR) analysis showing the change of β-sheet content in different recrystallization time. (d, e) Cumulative release curve of MAP and KGN from PSF and KSF with different β-sheet fraction respectively. (f) The contact angle with water of SF with different β-sheet fraction showing the wetting property. (g) The degradation of SF with different β-sheet fraction. (h) Comparison of release kinetics for drug releasing strategies between our approach (red line) and previous studies reported . The illustration showed the comparison of release kinetics for MSC recruiting strategies between our approach (gray area) and previous studies (purple points). (i) In vivo retention of PSF microspheres injected in the joint cavity visualized by in vivo <t>imaging</t> <t>system</t> (IVIS). (j) Fluorescent intensity (Taking the base-10 logarithm) at different timepoint after injection. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
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Characterization of microspheres. (a) Scanning electronic microscopic (SEM) observations shows the morphology and surface structure of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h. (b) Fourier transform infrared spectroscopy (FTIR) analysis showing the different second structure of SF (β-sheet, random coil/helix and β-turn) <t>content</t> in different recrystallization time. (c) Matched curve of β-sheet fraction with assembly time. Illustrations showed the morphology of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h, as well as the Fourier transform infrared spectroscopy (FTIR) analysis showing the change of β-sheet content in different recrystallization time. (d, e) Cumulative release curve of MAP and KGN from PSF and KSF with different β-sheet fraction respectively. (f) The contact angle with water of SF with different β-sheet fraction showing the wetting property. (g) The degradation of SF with different β-sheet fraction. (h) Comparison of release kinetics for drug releasing strategies between our approach (red line) and previous studies reported . The illustration showed the comparison of release kinetics for MSC recruiting strategies between our approach (gray area) and previous studies (purple points). (i) In vivo retention of PSF microspheres injected in the joint cavity visualized by in vivo <t>imaging</t> <t>system</t> (IVIS). (j) Fluorescent intensity (Taking the base-10 logarithm) at different timepoint after injection. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
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Characterization of microspheres. (a) Scanning electronic microscopic (SEM) observations shows the morphology and surface structure of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h. (b) Fourier transform infrared spectroscopy (FTIR) analysis showing the different second structure of SF (β-sheet, random coil/helix and β-turn) <t>content</t> in different recrystallization time. (c) Matched curve of β-sheet fraction with assembly time. Illustrations showed the morphology of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h, as well as the Fourier transform infrared spectroscopy (FTIR) analysis showing the change of β-sheet content in different recrystallization time. (d, e) Cumulative release curve of MAP and KGN from PSF and KSF with different β-sheet fraction respectively. (f) The contact angle with water of SF with different β-sheet fraction showing the wetting property. (g) The degradation of SF with different β-sheet fraction. (h) Comparison of release kinetics for drug releasing strategies between our approach (red line) and previous studies reported . The illustration showed the comparison of release kinetics for MSC recruiting strategies between our approach (gray area) and previous studies (purple points). (i) In vivo retention of PSF microspheres injected in the joint cavity visualized by in vivo <t>imaging</t> <t>system</t> (IVIS). (j) Fluorescent intensity (Taking the base-10 logarithm) at different timepoint after injection. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
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Characterization of microspheres. (a) Scanning electronic microscopic (SEM) observations shows the morphology and surface structure of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h. (b) Fourier transform infrared spectroscopy (FTIR) analysis showing the different second structure of SF (β-sheet, random coil/helix and β-turn) content in different recrystallization time. (c) Matched curve of β-sheet fraction with assembly time. Illustrations showed the morphology of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h, as well as the Fourier transform infrared spectroscopy (FTIR) analysis showing the change of β-sheet content in different recrystallization time. (d, e) Cumulative release curve of MAP and KGN from PSF and KSF with different β-sheet fraction respectively. (f) The contact angle with water of SF with different β-sheet fraction showing the wetting property. (g) The degradation of SF with different β-sheet fraction. (h) Comparison of release kinetics for drug releasing strategies between our approach (red line) and previous studies reported . The illustration showed the comparison of release kinetics for MSC recruiting strategies between our approach (gray area) and previous studies (purple points). (i) In vivo retention of PSF microspheres injected in the joint cavity visualized by in vivo imaging system (IVIS). (j) Fluorescent intensity (Taking the base-10 logarithm) at different timepoint after injection. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Journal: Bioactive Materials

Article Title: Precisely regulated physically-crosslinked carriers enable synergetic release of bioactive factors for MSC-mediated cartilage regeneration

doi: 10.1016/j.bioactmat.2026.01.009

Figure Lengend Snippet: Characterization of microspheres. (a) Scanning electronic microscopic (SEM) observations shows the morphology and surface structure of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h. (b) Fourier transform infrared spectroscopy (FTIR) analysis showing the different second structure of SF (β-sheet, random coil/helix and β-turn) content in different recrystallization time. (c) Matched curve of β-sheet fraction with assembly time. Illustrations showed the morphology of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h, as well as the Fourier transform infrared spectroscopy (FTIR) analysis showing the change of β-sheet content in different recrystallization time. (d, e) Cumulative release curve of MAP and KGN from PSF and KSF with different β-sheet fraction respectively. (f) The contact angle with water of SF with different β-sheet fraction showing the wetting property. (g) The degradation of SF with different β-sheet fraction. (h) Comparison of release kinetics for drug releasing strategies between our approach (red line) and previous studies reported . The illustration showed the comparison of release kinetics for MSC recruiting strategies between our approach (gray area) and previous studies (purple points). (i) In vivo retention of PSF microspheres injected in the joint cavity visualized by in vivo imaging system (IVIS). (j) Fluorescent intensity (Taking the base-10 logarithm) at different timepoint after injection. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Article Snippet: Immunofluorescent staining was performed with Aggrecan primary antibody (13880-1-AP, Proteintech, USA), ActinGreen ( R37110 , Thermo, USA) and DAPI (Solarbio, China) and observed with 3D reconstruction under high content imaging system (PerkinElmer, Operetta CLS, USA).

Techniques: Recrystallization, Fourier Transform Infrared Spectroscopy, Spectroscopy, Comparison, In Vivo, Injection, In Vivo Imaging