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A. Editing frequencies at the EPOR-sg1 locus measured at Day 4 post-electroporation with CBE6 mRNA prepared under different conditions, including capping strategies <t>(CleanCap</t> AG), incorporation of modified nucleotides (e.g., pseudouridine), RNase inhibitor addition, freeze-thaw cycles, and storage duration. B. Heatmaps showing EPOR -sg1 editing frequencies across varying CBE6 mRNA doses (2-10 µg) and electroporation conditions using Lonza 4D nucleofector with P3 buffer + DZ-100 code or B1mix buffer + CM-137 code. Data are shown as replicate measurements from a single donor (n = 1).
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A. Editing frequencies at the EPOR-sg1 locus measured at Day 4 post-electroporation with CBE6 mRNA prepared under different conditions, including capping strategies <t>(CleanCap</t> AG), incorporation of modified nucleotides (e.g., pseudouridine), RNase inhibitor addition, freeze-thaw cycles, and storage duration. B. Heatmaps showing EPOR -sg1 editing frequencies across varying CBE6 mRNA doses (2-10 µg) and electroporation conditions using Lonza 4D nucleofector with P3 buffer + DZ-100 code or B1mix buffer + CM-137 code. Data are shown as replicate measurements from a single donor (n = 1).
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A. Editing frequencies at the EPOR-sg1 locus measured at Day 4 post-electroporation with CBE6 mRNA prepared under different conditions, including capping strategies (CleanCap AG), incorporation of modified nucleotides (e.g., pseudouridine), RNase inhibitor addition, freeze-thaw cycles, and storage duration. B. Heatmaps showing EPOR -sg1 editing frequencies across varying CBE6 mRNA doses (2-10 µg) and electroporation conditions using Lonza 4D nucleofector with P3 buffer + DZ-100 code or B1mix buffer + CM-137 code. Data are shown as replicate measurements from a single donor (n = 1).

Journal: bioRxiv

Article Title: Combinatorial base editing couples disease correction with lineage amplification in hematopoietic stem and progenitor cells

doi: 10.64898/2026.04.13.718029

Figure Lengend Snippet: A. Editing frequencies at the EPOR-sg1 locus measured at Day 4 post-electroporation with CBE6 mRNA prepared under different conditions, including capping strategies (CleanCap AG), incorporation of modified nucleotides (e.g., pseudouridine), RNase inhibitor addition, freeze-thaw cycles, and storage duration. B. Heatmaps showing EPOR -sg1 editing frequencies across varying CBE6 mRNA doses (2-10 µg) and electroporation conditions using Lonza 4D nucleofector with P3 buffer + DZ-100 code or B1mix buffer + CM-137 code. Data are shown as replicate measurements from a single donor (n = 1).

Article Snippet: In vitro transcription (IVT) was performed using the HiScribe T7 High Yield RNA Synthesis Kit or the HiScribe T7 mRNA Kit with CleanCap AG (New England Biolabs; E2040 or E2080).

Techniques: Electroporation, Modification