Review





Similar Products

clcbio software  (CLC Bio)
90
CLC Bio clcbio software
Different stages during transformation with A. tumefaciens , either carrying <t>pCAMBIA1300</t> ( hptII ) or no T-DNA (WT), and expression in mature and imbibed embryos, are shown. The following stages of callus are shown: 6 weeks after induction of embryogenic callus; immediately after 15 min infection with A. tumefaciens ; after a 3-day incubation in R2CS medium; after a 2-week incubation in R2S selection medium; 3 weeks after transfer to NBS selection medium; after an 8 to 10-day incubation in PR-AG selection medium; 3–4 weeks after induction of regeneration in RN medium; and two weeks after seedling culture in P medium. Embryos extracted from mature seeds, either before or after imbibition in water for two or five hours, were also analyzed. When the hptII DNA cassette was transformed, transgenic cells were selected using hygromycin (asterisk). Three biological replicates, each of five calluses, with two experimental replicates, were analyzed at each stage. The ef1 α reference gene (M values <0.5 in these samples) was used for normalization. Dark to light color scale represents high to low expression level. All values were in the 90 to 0.01-fold range. nd: not determined. Striped: underestimated values due to callus samples containing a mixture of transgenic and non-transgenic cells.
Clcbio Software, supplied by CLC Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/clcbio software/product/CLC Bio
Average 90 stars, based on 1 article reviews
clcbio software - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
clcbio main workbench software version 5.7.1  (Qiagen)
90
Qiagen clcbio main workbench software version 5.7.1
Different stages during transformation with A. tumefaciens , either carrying <t>pCAMBIA1300</t> ( hptII ) or no T-DNA (WT), and expression in mature and imbibed embryos, are shown. The following stages of callus are shown: 6 weeks after induction of embryogenic callus; immediately after 15 min infection with A. tumefaciens ; after a 3-day incubation in R2CS medium; after a 2-week incubation in R2S selection medium; 3 weeks after transfer to NBS selection medium; after an 8 to 10-day incubation in PR-AG selection medium; 3–4 weeks after induction of regeneration in RN medium; and two weeks after seedling culture in P medium. Embryos extracted from mature seeds, either before or after imbibition in water for two or five hours, were also analyzed. When the hptII DNA cassette was transformed, transgenic cells were selected using hygromycin (asterisk). Three biological replicates, each of five calluses, with two experimental replicates, were analyzed at each stage. The ef1 α reference gene (M values <0.5 in these samples) was used for normalization. Dark to light color scale represents high to low expression level. All values were in the 90 to 0.01-fold range. nd: not determined. Striped: underestimated values due to callus samples containing a mixture of transgenic and non-transgenic cells.
Clcbio Main Workbench Software Version 5.7.1, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/clcbio main workbench software version 5.7.1/product/Qiagen
Average 90 stars, based on 1 article reviews
clcbio main workbench software version 5.7.1 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
commercial software clcbio  (CLC Bio)
90
CLC Bio commercial software clcbio
Different stages during transformation with A. tumefaciens , either carrying <t>pCAMBIA1300</t> ( hptII ) or no T-DNA (WT), and expression in mature and imbibed embryos, are shown. The following stages of callus are shown: 6 weeks after induction of embryogenic callus; immediately after 15 min infection with A. tumefaciens ; after a 3-day incubation in R2CS medium; after a 2-week incubation in R2S selection medium; 3 weeks after transfer to NBS selection medium; after an 8 to 10-day incubation in PR-AG selection medium; 3–4 weeks after induction of regeneration in RN medium; and two weeks after seedling culture in P medium. Embryos extracted from mature seeds, either before or after imbibition in water for two or five hours, were also analyzed. When the hptII DNA cassette was transformed, transgenic cells were selected using hygromycin (asterisk). Three biological replicates, each of five calluses, with two experimental replicates, were analyzed at each stage. The ef1 α reference gene (M values <0.5 in these samples) was used for normalization. Dark to light color scale represents high to low expression level. All values were in the 90 to 0.01-fold range. nd: not determined. Striped: underestimated values due to callus samples containing a mixture of transgenic and non-transgenic cells.
Commercial Software Clcbio, supplied by CLC Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/commercial software clcbio/product/CLC Bio
Average 90 stars, based on 1 article reviews
commercial software clcbio - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


Different stages during transformation with A. tumefaciens , either carrying pCAMBIA1300 ( hptII ) or no T-DNA (WT), and expression in mature and imbibed embryos, are shown. The following stages of callus are shown: 6 weeks after induction of embryogenic callus; immediately after 15 min infection with A. tumefaciens ; after a 3-day incubation in R2CS medium; after a 2-week incubation in R2S selection medium; 3 weeks after transfer to NBS selection medium; after an 8 to 10-day incubation in PR-AG selection medium; 3–4 weeks after induction of regeneration in RN medium; and two weeks after seedling culture in P medium. Embryos extracted from mature seeds, either before or after imbibition in water for two or five hours, were also analyzed. When the hptII DNA cassette was transformed, transgenic cells were selected using hygromycin (asterisk). Three biological replicates, each of five calluses, with two experimental replicates, were analyzed at each stage. The ef1 α reference gene (M values <0.5 in these samples) was used for normalization. Dark to light color scale represents high to low expression level. All values were in the 90 to 0.01-fold range. nd: not determined. Striped: underestimated values due to callus samples containing a mixture of transgenic and non-transgenic cells.

Journal: PLoS ONE

Article Title: Production of Phytotoxic Cationic α-Helical Antimicrobial Peptides in Plant Cells Using Inducible Promoters

doi: 10.1371/journal.pone.0109990

Figure Lengend Snippet: Different stages during transformation with A. tumefaciens , either carrying pCAMBIA1300 ( hptII ) or no T-DNA (WT), and expression in mature and imbibed embryos, are shown. The following stages of callus are shown: 6 weeks after induction of embryogenic callus; immediately after 15 min infection with A. tumefaciens ; after a 3-day incubation in R2CS medium; after a 2-week incubation in R2S selection medium; 3 weeks after transfer to NBS selection medium; after an 8 to 10-day incubation in PR-AG selection medium; 3–4 weeks after induction of regeneration in RN medium; and two weeks after seedling culture in P medium. Embryos extracted from mature seeds, either before or after imbibition in water for two or five hours, were also analyzed. When the hptII DNA cassette was transformed, transgenic cells were selected using hygromycin (asterisk). Three biological replicates, each of five calluses, with two experimental replicates, were analyzed at each stage. The ef1 α reference gene (M values <0.5 in these samples) was used for normalization. Dark to light color scale represents high to low expression level. All values were in the 90 to 0.01-fold range. nd: not determined. Striped: underestimated values due to callus samples containing a mixture of transgenic and non-transgenic cells.

Article Snippet: On sequence verification (Macrogen, Seoul, Korea) using the CLCbio software (Aarhus, Denmark), the complete constructs, bp100.2 , bp100-dsred-tag54 and dsred-tag54 plus promoter and terminator elements, were directionally inserted into the Kpn I and Sbf I sites of pCAMBIA1300.

Techniques: Transformation Assay, Expressing, Infection, Incubation, Selection, Transgenic Assay