Journal: PLOS One

Article Title: Patient-derived organoids predict chemotherapy response of locally advanced gastric cancer

doi: 10.1371/journal.pone.0339416

Figure Lengend Snippet: (A) Schematic overview of GC PDOs experimental workflow: tissue isolation, organoid culture, histopathological evaluation, genomic analysis, in vitro drug sensitivity testing, and prediction of clinical response. (B) Representative bright-field images of GC-010 PDOs from day 0 to day 9 post-seeding in Matrigel. Scale bars, 100 µm. Similar growth patterns were observed across all 17 established PDO lines. (C) Bright-field images highlighting the morphological diversity among PDOs derived from SRCC (GC-014, GC-027) and non-SRCC (GC-010, GC-011). Scale bars, 100 µm. (D) Representative H&E staining images of GC PDOs and their corresponding parental tumors. GC-007 is SRCC and GC-025 is non-SRCC. Black arrow indicates signet ring cells; blue arrow indicates solid or cystic structures. Scale bars, 50 µm. (E) IHC staining of Ki67, CK7, CK20 and CDX2 in GC PDOs and their parental tumors from GC-007 and GC-025. GC PDOs faithfully recapitulated histologic features of their parental tumors. Scale bars, 50 µm. P, passage. D, day. GC, gastric cancer. PDOs, patient-derived organoids. SRCC, signet-ring cell carcinoma. Non-SRCC, non-signet-ring cell carcinoma.

Article Snippet: The primary antibodies used for IHC included Ki67 (clone SP6, 1:600 dilution, Servicebio, Wuhan, China), CK7 (clone 7D8G8, 1:600 dilution, Servicebio, Wuhan, China), CDX2 (clone 4H2A8, 1:1500 dilution, Servicebio, Wuhan, China), and CK20 (clone SP33, 1:1500 dilution, Servicebio, Wuhan, China).

Techniques: Isolation, In Vitro, Derivative Assay, Staining, Immunohistochemistry

Journal: bioRxiv

Article Title: Single-cell Analysis of Paired FFPE Placentas Reveals Trophoblast Reprogramming and Immune Dysregulation in Chronic Villitis of Unknown Etiology

doi: 10.64898/2026.02.28.708431

Figure Lengend Snippet: (A) Expression of MHC class I and II molecules in VUE trophoblasts compared to controls. (B) UMAP visualization of trophoblast re-clustering, identifying 8 distinct subpopulations. (C) Bubble chart of MHC expression across subclusters. (D) Stacked bar plot of relative cellular composition of each trophoblasts cell subset. (E) Box plots of the trophoblasts cell subset proportions between Control and VUE. Red arrows indicate expanded populations, while green arrows indicate depleted populations. (F) Volcano plots of trophoblasts cell subset differentially expressed genes. (G) Gene Set Enrichment analysis of EVT_2 and STB_2. (H) Multiplex immunohistochemistry (mIHC) showing CK7 + (green) HLA-A/B + (magenta) trophoblasts in VUE tissue (white arrows).

Article Snippet: For multiplex immunohistochemical staining, primary antibodies are against human CK7 (17513, Proteintech), and HLA-A/B (15240-1-AP, Proteintech), the Four Detect Kit for Rabbit Primary Antibody kit (PK10033, Proteintech) used for multiple staining.

Techniques: Expressing, Control, Multiplex Assay, Immunohistochemistry