Journal: Nature communications

Article Title: A genome-wide CRISPR screen identifies WDFY3 as a regulator of macrophage efferocytosis.

doi: 10.1038/s41467-022-35604-8

Figure Lengend Snippet: Fig. 4 | The C-terminal WDFY3 is sufficient for regulating degradation yet the full-length WDFY3 is required for the uptake of ACs during efferocytosis. a Schematics of lentiviral overexpression of C-terminal WDFY3 in BMDMs of Cre-

Article Snippet: Preparation of sheep red blood cells (RBCs) for efferocytosis Sheep red blood cells (RBCs) (Rockland) were obtained.

Techniques: Over Expression

Journal: Nature communications

Article Title: A genome-wide CRISPR screen identifies WDFY3 as a regulator of macrophage efferocytosis.

doi: 10.1038/s41467-022-35604-8

Figure Lengend Snippet: Fig. 5 | Mice with myeloid Wdfy3 knockout show impaired efferocytosis in vivo. a Schematics of experimental design for in vivo thymus efferocytosis assay. b Thymus weight. c Total number of cells per thymus. d Percentage of F4/80+

Article Snippet: Preparation of sheep red blood cells (RBCs) for efferocytosis Sheep red blood cells (RBCs) (Rockland) were obtained.

Techniques: Knock-Out, In Vivo

Journal: Nature communications

Article Title: A genome-wide CRISPR screen identifies WDFY3 as a regulator of macrophage efferocytosis.

doi: 10.1038/s41467-022-35604-8

Figure Lengend Snippet: Fig. 7 | Schematic figure summarizing how WDFY3 regulates macrophage efferocytosis. WDFY3 is discovered as a new regulator of efferocytosis by macro- phages. WDFY3 deficiency in macrophages specifically impaired uptake, not binding, of apoptotic cells due to defective actin depolymerization, thus phago- some formation. WDFY3 directly interacts with GABARAP, one of the seven mem- bers of the LC3/GABARAP protein family, to facilitate LC3 lipidation and the subsequent phagosome-lysosome fusion and degradation of the engulfed AC components.

Article Snippet: Preparation of sheep red blood cells (RBCs) for efferocytosis Sheep red blood cells (RBCs) (Rockland) were obtained.

Techniques: Binding Assay