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tagmentation cut t ag chromatin profiling paradigm  (EpiCypher)
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EpiCypher tagmentation cut t ag chromatin profiling paradigm
(A) Browser snapshot <t>of</t> <t>CUT&Tag</t> profiling in K562 cells for the H3K27me3, H3K27me2, H3K27me1, and H3K27ac modifications, and RNAPIIS2/5p at the POU5F1 (OCT4) locus. A Polycomb domain overlaps the PSOR1S1C gene. (B) (top) Pearson correlation heatmap of active RNAPII versus H3K27 modifications at coding genes including a 1 kilobase promoter region upstream (n=19,946). (bottom) Signal heatmap of correlated gene values after log2 transformation. Genes in annotated Polycomb domains are highlighted in red and show high H3K27me3, low active RNAPII signal. (C) Gating schema based on DRAQ5 measurements of DNA-content used to isolate S-phase fractions via fluorescence-activated cell sorting (FACS). (D) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis) from (C). Signal is shown as fraction of the maximum averaged across two replicates. Log10(max RPKM) per gene is shown in purple for the H3K27me1, H3K27me2, and H3K27me3 modifications to demonstrate high H3K27me3 signal and relatively less H3K27me2 and H3K27me1 signal at these genes.
Tagmentation Cut T Ag Chromatin Profiling Paradigm, supplied by EpiCypher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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simplechip plus sonication chromatin ip kit  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc simplechip plus sonication chromatin ip kit
(A) Browser snapshot <t>of</t> <t>CUT&Tag</t> profiling in K562 cells for the H3K27me3, H3K27me2, H3K27me1, and H3K27ac modifications, and RNAPIIS2/5p at the POU5F1 (OCT4) locus. A Polycomb domain overlaps the PSOR1S1C gene. (B) (top) Pearson correlation heatmap of active RNAPII versus H3K27 modifications at coding genes including a 1 kilobase promoter region upstream (n=19,946). (bottom) Signal heatmap of correlated gene values after log2 transformation. Genes in annotated Polycomb domains are highlighted in red and show high H3K27me3, low active RNAPII signal. (C) Gating schema based on DRAQ5 measurements of DNA-content used to isolate S-phase fractions via fluorescence-activated cell sorting (FACS). (D) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis) from (C). Signal is shown as fraction of the maximum averaged across two replicates. Log10(max RPKM) per gene is shown in purple for the H3K27me1, H3K27me2, and H3K27me3 modifications to demonstrate high H3K27me3 signal and relatively less H3K27me2 and H3K27me1 signal at these genes.
Simplechip Plus Sonication Chromatin Ip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/simplechip plus sonication chromatin ip kit/product/Cell Signaling Technology Inc
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simplechip plus sonication chromatin ip kit - by Bioz Stars, 2026-05
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chromatin  (Janssen)
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Janssen chromatin
(A) Browser snapshot <t>of</t> <t>CUT&Tag</t> profiling in K562 cells for the H3K27me3, H3K27me2, H3K27me1, and H3K27ac modifications, and RNAPIIS2/5p at the POU5F1 (OCT4) locus. A Polycomb domain overlaps the PSOR1S1C gene. (B) (top) Pearson correlation heatmap of active RNAPII versus H3K27 modifications at coding genes including a 1 kilobase promoter region upstream (n=19,946). (bottom) Signal heatmap of correlated gene values after log2 transformation. Genes in annotated Polycomb domains are highlighted in red and show high H3K27me3, low active RNAPII signal. (C) Gating schema based on DRAQ5 measurements of DNA-content used to isolate S-phase fractions via fluorescence-activated cell sorting (FACS). (D) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis) from (C). Signal is shown as fraction of the maximum averaged across two replicates. Log10(max RPKM) per gene is shown in purple for the H3K27me1, H3K27me2, and H3K27me3 modifications to demonstrate high H3K27me3 signal and relatively less H3K27me2 and H3K27me1 signal at these genes.
Chromatin, supplied by Janssen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chromatin immunoprecipitation sequencing chip seq analysis  (Macrogen)
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Macrogen chromatin immunoprecipitation sequencing chip seq analysis
(A) Browser snapshot <t>of</t> <t>CUT&Tag</t> profiling in K562 cells for the H3K27me3, H3K27me2, H3K27me1, and H3K27ac modifications, and RNAPIIS2/5p at the POU5F1 (OCT4) locus. A Polycomb domain overlaps the PSOR1S1C gene. (B) (top) Pearson correlation heatmap of active RNAPII versus H3K27 modifications at coding genes including a 1 kilobase promoter region upstream (n=19,946). (bottom) Signal heatmap of correlated gene values after log2 transformation. Genes in annotated Polycomb domains are highlighted in red and show high H3K27me3, low active RNAPII signal. (C) Gating schema based on DRAQ5 measurements of DNA-content used to isolate S-phase fractions via fluorescence-activated cell sorting (FACS). (D) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis) from (C). Signal is shown as fraction of the maximum averaged across two replicates. Log10(max RPKM) per gene is shown in purple for the H3K27me1, H3K27me2, and H3K27me3 modifications to demonstrate high H3K27me3 signal and relatively less H3K27me2 and H3K27me1 signal at these genes.
Chromatin Immunoprecipitation Sequencing Chip Seq Analysis, supplied by Macrogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna chromatin contact data rna chrom  (Chrom Tech)
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Chrom Tech rna chromatin contact data rna chrom
(A) Browser snapshot <t>of</t> <t>CUT&Tag</t> profiling in K562 cells for the H3K27me3, H3K27me2, H3K27me1, and H3K27ac modifications, and RNAPIIS2/5p at the POU5F1 (OCT4) locus. A Polycomb domain overlaps the PSOR1S1C gene. (B) (top) Pearson correlation heatmap of active RNAPII versus H3K27 modifications at coding genes including a 1 kilobase promoter region upstream (n=19,946). (bottom) Signal heatmap of correlated gene values after log2 transformation. Genes in annotated Polycomb domains are highlighted in red and show high H3K27me3, low active RNAPII signal. (C) Gating schema based on DRAQ5 measurements of DNA-content used to isolate S-phase fractions via fluorescence-activated cell sorting (FACS). (D) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis) from (C). Signal is shown as fraction of the maximum averaged across two replicates. Log10(max RPKM) per gene is shown in purple for the H3K27me1, H3K27me2, and H3K27me3 modifications to demonstrate high H3K27me3 signal and relatively less H3K27me2 and H3K27me1 signal at these genes.
Rna Chromatin Contact Data Rna Chrom, supplied by Chrom Tech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna chromatin contact data rna chromatin contacts  (Chrom Tech)
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Chrom Tech rna chromatin contact data rna chromatin contacts
(A) Browser snapshot <t>of</t> <t>CUT&Tag</t> profiling in K562 cells for the H3K27me3, H3K27me2, H3K27me1, and H3K27ac modifications, and RNAPIIS2/5p at the POU5F1 (OCT4) locus. A Polycomb domain overlaps the PSOR1S1C gene. (B) (top) Pearson correlation heatmap of active RNAPII versus H3K27 modifications at coding genes including a 1 kilobase promoter region upstream (n=19,946). (bottom) Signal heatmap of correlated gene values after log2 transformation. Genes in annotated Polycomb domains are highlighted in red and show high H3K27me3, low active RNAPII signal. (C) Gating schema based on DRAQ5 measurements of DNA-content used to isolate S-phase fractions via fluorescence-activated cell sorting (FACS). (D) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis) from (C). Signal is shown as fraction of the maximum averaged across two replicates. Log10(max RPKM) per gene is shown in purple for the H3K27me1, H3K27me2, and H3K27me3 modifications to demonstrate high H3K27me3 signal and relatively less H3K27me2 and H3K27me1 signal at these genes.
Rna Chromatin Contact Data Rna Chromatin Contacts, supplied by Chrom Tech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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snhg1 rna chromatin contacts  (Chrom Tech)
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Chrom Tech snhg1 rna chromatin contacts
(A) Browser snapshot <t>of</t> <t>CUT&Tag</t> profiling in K562 cells for the H3K27me3, H3K27me2, H3K27me1, and H3K27ac modifications, and RNAPIIS2/5p at the POU5F1 (OCT4) locus. A Polycomb domain overlaps the PSOR1S1C gene. (B) (top) Pearson correlation heatmap of active RNAPII versus H3K27 modifications at coding genes including a 1 kilobase promoter region upstream (n=19,946). (bottom) Signal heatmap of correlated gene values after log2 transformation. Genes in annotated Polycomb domains are highlighted in red and show high H3K27me3, low active RNAPII signal. (C) Gating schema based on DRAQ5 measurements of DNA-content used to isolate S-phase fractions via fluorescence-activated cell sorting (FACS). (D) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis) from (C). Signal is shown as fraction of the maximum averaged across two replicates. Log10(max RPKM) per gene is shown in purple for the H3K27me1, H3K27me2, and H3K27me3 modifications to demonstrate high H3K27me3 signal and relatively less H3K27me2 and H3K27me1 signal at these genes.
Snhg1 Rna Chromatin Contacts, supplied by Chrom Tech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/snhg1 rna chromatin contacts/product/Chrom Tech
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simplechip enzymatic chromatin ip kit  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc simplechip enzymatic chromatin ip kit
(A) Browser snapshot <t>of</t> <t>CUT&Tag</t> profiling in K562 cells for the H3K27me3, H3K27me2, H3K27me1, and H3K27ac modifications, and RNAPIIS2/5p at the POU5F1 (OCT4) locus. A Polycomb domain overlaps the PSOR1S1C gene. (B) (top) Pearson correlation heatmap of active RNAPII versus H3K27 modifications at coding genes including a 1 kilobase promoter region upstream (n=19,946). (bottom) Signal heatmap of correlated gene values after log2 transformation. Genes in annotated Polycomb domains are highlighted in red and show high H3K27me3, low active RNAPII signal. (C) Gating schema based on DRAQ5 measurements of DNA-content used to isolate S-phase fractions via fluorescence-activated cell sorting (FACS). (D) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis) from (C). Signal is shown as fraction of the maximum averaged across two replicates. Log10(max RPKM) per gene is shown in purple for the H3K27me1, H3K27me2, and H3K27me3 modifications to demonstrate high H3K27me3 signal and relatively less H3K27me2 and H3K27me1 signal at these genes.
Simplechip Enzymatic Chromatin Ip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/simplechip enzymatic chromatin ip kit/product/Cell Signaling Technology Inc
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simplechip enzymatic chromatin ip kit - by Bioz Stars, 2026-05
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9003s  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc 9003s
(A) Browser snapshot <t>of</t> <t>CUT&Tag</t> profiling in K562 cells for the H3K27me3, H3K27me2, H3K27me1, and H3K27ac modifications, and RNAPIIS2/5p at the POU5F1 (OCT4) locus. A Polycomb domain overlaps the PSOR1S1C gene. (B) (top) Pearson correlation heatmap of active RNAPII versus H3K27 modifications at coding genes including a 1 kilobase promoter region upstream (n=19,946). (bottom) Signal heatmap of correlated gene values after log2 transformation. Genes in annotated Polycomb domains are highlighted in red and show high H3K27me3, low active RNAPII signal. (C) Gating schema based on DRAQ5 measurements of DNA-content used to isolate S-phase fractions via fluorescence-activated cell sorting (FACS). (D) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis) from (C). Signal is shown as fraction of the maximum averaged across two replicates. Log10(max RPKM) per gene is shown in purple for the H3K27me1, H3K27me2, and H3K27me3 modifications to demonstrate high H3K27me3 signal and relatively less H3K27me2 and H3K27me1 signal at these genes.
9003s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/9003s/product/Cell Signaling Technology Inc
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9003s - by Bioz Stars, 2026-05
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Image Search Results


(A) Browser snapshot of CUT&Tag profiling in K562 cells for the H3K27me3, H3K27me2, H3K27me1, and H3K27ac modifications, and RNAPIIS2/5p at the POU5F1 (OCT4) locus. A Polycomb domain overlaps the PSOR1S1C gene. (B) (top) Pearson correlation heatmap of active RNAPII versus H3K27 modifications at coding genes including a 1 kilobase promoter region upstream (n=19,946). (bottom) Signal heatmap of correlated gene values after log2 transformation. Genes in annotated Polycomb domains are highlighted in red and show high H3K27me3, low active RNAPII signal. (C) Gating schema based on DRAQ5 measurements of DNA-content used to isolate S-phase fractions via fluorescence-activated cell sorting (FACS). (D) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis) from (C). Signal is shown as fraction of the maximum averaged across two replicates. Log10(max RPKM) per gene is shown in purple for the H3K27me1, H3K27me2, and H3K27me3 modifications to demonstrate high H3K27me3 signal and relatively less H3K27me2 and H3K27me1 signal at these genes.

Journal: bioRxiv

Article Title: Histone H3K27 methylation states are sequentially catalyzed in cycling cells

doi: 10.64898/2026.04.30.721988

Figure Lengend Snippet: (A) Browser snapshot of CUT&Tag profiling in K562 cells for the H3K27me3, H3K27me2, H3K27me1, and H3K27ac modifications, and RNAPIIS2/5p at the POU5F1 (OCT4) locus. A Polycomb domain overlaps the PSOR1S1C gene. (B) (top) Pearson correlation heatmap of active RNAPII versus H3K27 modifications at coding genes including a 1 kilobase promoter region upstream (n=19,946). (bottom) Signal heatmap of correlated gene values after log2 transformation. Genes in annotated Polycomb domains are highlighted in red and show high H3K27me3, low active RNAPII signal. (C) Gating schema based on DRAQ5 measurements of DNA-content used to isolate S-phase fractions via fluorescence-activated cell sorting (FACS). (D) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis) from (C). Signal is shown as fraction of the maximum averaged across two replicates. Log10(max RPKM) per gene is shown in purple for the H3K27me1, H3K27me2, and H3K27me3 modifications to demonstrate high H3K27me3 signal and relatively less H3K27me2 and H3K27me1 signal at these genes.

Article Snippet: Confirmation of H3K27 methylation profiling with designer barcoded nucleosomes: (A) Cleavage Under Targets & Tagmentation (CUT&Tag) chromatin profiling paradigm with a synthetic nucleosome spike-in panel (EpiCypher, Cat# 19-1002).

Techniques: Transformation Assay, Fluorescence, FACS

(A) Experimental design for 8-hour treatment of cycling K562 cells with small-molecule inhibitors of PRC2. (B) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis). Signal is shown as fraction of the maximum averaged across two replicates. Max RPKM is computed per treatment across the S-phase time course. Log10(max RPKM) per gene is show in purple for active RNAPII, H3K27ac, H3K27me1, H3K27me2, and H3K27me3 (left to right). The heatmaps show signal after treatment for 8 hours with DMSO (vehicle control, top), the EZH2 inhibitor EPZ-6438 (middle), and the EED inhibitor EED-226 (bottom). (C) Statistical paradigm for testing the relationship between drug effects versus replication timing at genes in Polycomb domains. Scatter plots are from Supplementary Figure 8. Genes above an absolute of log2 fold-change of 0.2 are colored red for up in treatment or blue for down in treatment, or grey for Log2 fold-change < 0.2. (D) Linear regression analysis (y-axis) of response to EPZ-6438 (top), and EED-226 (bottom) within S-phase fractions (x-axis) versus replication timing at genes in Polycomb domains. Stars indicate a significance < 0.001 after FDR correction. Bar color is Spearman’s rho (E) PRC2 inhibition at early replicating genes in and outside Polycomb domains. Median log2 fold-change for EPZ-6438 (top) and EED-226 (bottom) versus DMSO is shown for H3K27me2 (y-axis) and H3K27ac (x-axis) in the S2 fraction. The fold difference between Log2 fold-change in the S1 versus S2 fraction is shown for differences greater than ±0.2. Stars indicate a significant difference in Log2 fold-change values by the Wilcoxon test with a p-value < 0.001 after Bonferroni correction.

Journal: bioRxiv

Article Title: Histone H3K27 methylation states are sequentially catalyzed in cycling cells

doi: 10.64898/2026.04.30.721988

Figure Lengend Snippet: (A) Experimental design for 8-hour treatment of cycling K562 cells with small-molecule inhibitors of PRC2. (B) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis). Signal is shown as fraction of the maximum averaged across two replicates. Max RPKM is computed per treatment across the S-phase time course. Log10(max RPKM) per gene is show in purple for active RNAPII, H3K27ac, H3K27me1, H3K27me2, and H3K27me3 (left to right). The heatmaps show signal after treatment for 8 hours with DMSO (vehicle control, top), the EZH2 inhibitor EPZ-6438 (middle), and the EED inhibitor EED-226 (bottom). (C) Statistical paradigm for testing the relationship between drug effects versus replication timing at genes in Polycomb domains. Scatter plots are from Supplementary Figure 8. Genes above an absolute of log2 fold-change of 0.2 are colored red for up in treatment or blue for down in treatment, or grey for Log2 fold-change < 0.2. (D) Linear regression analysis (y-axis) of response to EPZ-6438 (top), and EED-226 (bottom) within S-phase fractions (x-axis) versus replication timing at genes in Polycomb domains. Stars indicate a significance < 0.001 after FDR correction. Bar color is Spearman’s rho (E) PRC2 inhibition at early replicating genes in and outside Polycomb domains. Median log2 fold-change for EPZ-6438 (top) and EED-226 (bottom) versus DMSO is shown for H3K27me2 (y-axis) and H3K27ac (x-axis) in the S2 fraction. The fold difference between Log2 fold-change in the S1 versus S2 fraction is shown for differences greater than ±0.2. Stars indicate a significant difference in Log2 fold-change values by the Wilcoxon test with a p-value < 0.001 after Bonferroni correction.

Article Snippet: Confirmation of H3K27 methylation profiling with designer barcoded nucleosomes: (A) Cleavage Under Targets & Tagmentation (CUT&Tag) chromatin profiling paradigm with a synthetic nucleosome spike-in panel (EpiCypher, Cat# 19-1002).

Techniques: Control, Inhibition

(A) CUT&Tag signal at genes outside Polycomb domains enriched for H3K27me2 (top, n=2,268) and (B) H3K27me1 (bottom, n=3,872) ranked by replication timing (y-axis) over the S-phase fractions (x-axis). Signal is shown as fraction of the maximum (dilution) averaged across two replicates.

Journal: bioRxiv

Article Title: Histone H3K27 methylation states are sequentially catalyzed in cycling cells

doi: 10.64898/2026.04.30.721988

Figure Lengend Snippet: (A) CUT&Tag signal at genes outside Polycomb domains enriched for H3K27me2 (top, n=2,268) and (B) H3K27me1 (bottom, n=3,872) ranked by replication timing (y-axis) over the S-phase fractions (x-axis). Signal is shown as fraction of the maximum (dilution) averaged across two replicates.

Article Snippet: Confirmation of H3K27 methylation profiling with designer barcoded nucleosomes: (A) Cleavage Under Targets & Tagmentation (CUT&Tag) chromatin profiling paradigm with a synthetic nucleosome spike-in panel (EpiCypher, Cat# 19-1002).

Techniques: