Journal: Genes & Diseases

Article Title: 8-Chloroadenosine suppresses hepatocellular carcinoma progression via ADAR1/PPARγ axis-mediated lipid metabolism

doi: 10.1016/j.gendis.2025.101874

Figure Lengend Snippet: 8-Cl-Ado suppresses lipid metabolism in liver cancer by regulating gene expression. (A) The Venn diagram showing common differentially expressed genes (DEGs, p < 0.05) in HepG2 and Huh7 cells treated with 8-Cl-Ado for 24 h. (B) KEGG pathway enrichment analysis of common DEGs (top 10 pathways; 4 lipid metabolism-related pathways highlighted in red). (C) Effect of 8-Cl-Ado on lipid accumulation in HepG2 cells (Left: Representative images of oil red O staining; Right: Quantification of lipid droplet detection by oil red O staining). (D) Effect of 8-Cl-Ado on intracellular total cholesterol (CHO) and triglyceride (TG) levels in liver cancer cells. (E) Schematic diagram of CHO and fatty acid biosynthesis pathways. (F) The heatmap visualizing the expression patterns and significance (Log 2 (fold change)) of key genes involved in CHO biosynthesis and fatty acid metabolism ( n = 20) in 8-Cl-Ado-treated HepG2 and Huh7 cells. (G) Effect of 8-Cl-Ado on the expression levels of key genes involved in CHO biosynthesis and fatty acid metabolism in HepG2 cells ( n = 20). All experiments were performed with at least three biologically independent replicates ( n ≥ 3). Data were presented as mean ± standard error of the mean. Statistical significance was determined using Student's two-tailed t -test for two-group comparisons or one-way ANOVA for multiple groups. ∗∗∗ p < 0.001.

Article Snippet: The levels of cellular triglycerides and total cholesterol were investigated according to the manufacturer's instructions (Cat. No. 000180 and 000220; Nanjing Jiancheng Bioengineering Institute).

Techniques: Gene Expression, Staining, Expressing, Two Tailed Test

Journal: Poultry Science

Article Title: Overexpression of irisin reduces embryonic and diet-induced hepatic lipid accumulation in chickens

doi: 10.1016/j.psj.2026.106831

Figure Lengend Snippet: Lipid accumulation in the liver of 19-day chicken embryos was reduced by lentivirus-mediated overexpression of irisin. A Schematic diagram of the lentiviral vector structure. B Effects of lentivirus -mediated overexpression of irisin on chicken hatchability. Data analysis was performed using the chi-square test. C The levels of exogenous irisin in plasma were measured in 19-day-old chicken embryos. D Triglyceride (TG) and total cholesterol (TC) content in chicken embryonic liver. E H&E and Oil Red O staining of chicken embryonic liver. In the Control group, numerous lipid droplets were observed within hepatocytes, accompanied by extensive cytoplasmic vacuolization and indistinct hepatic cord architecture. Compared to the Control group, the Irisin group exhibited a significant reduction in lipid droplets, marked improvement in cytoplasmic vacuolization, and clear, intact hepatic cord structure. F Effects of irisin on the expression levels of lipid-related genes in chicken embryonic liver.

Article Snippet: Hepatic and plasma triglycerides (TG) and total cholesterol (TC) levels were measured using commercial kits (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer's protocols.

Techniques: Over Expression, Plasmid Preparation, Clinical Proteomics, Staining, Control, Expressing