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The release of chlorambucil from F127-folate@PLGA/CHL/IR780 and F127@PLGA/CHL/IR780 after 24, 48, 72, and 168 h of incubation in 0.1× PBS (NaCl 13.7 mM, KCl 0.27 mM, NaH 2 PO 4 1 mM, and KHPO 4 0.18 mM) at pH 7.4 and pH 5.4 at 37 °C; n = 3.

Journal: Beilstein Journal of Nanotechnology

Article Title: Therapeutic effect of F127-folate@PLGA/CHL/IR780 nanoparticles on folate receptor-expressing cancer cells

doi: 10.3762/bjnano.15.78

Figure Lengend Snippet: The release of chlorambucil from F127-folate@PLGA/CHL/IR780 and F127@PLGA/CHL/IR780 after 24, 48, 72, and 168 h of incubation in 0.1× PBS (NaCl 13.7 mM, KCl 0.27 mM, NaH 2 PO 4 1 mM, and KHPO 4 0.18 mM) at pH 7.4 and pH 5.4 at 37 °C; n = 3.

Article Snippet: Chlorambucil (C0253), PLGA 504H (719900), IR780 (425311), Pluronic™ F-127 (P2443), poly(vinyl alcohol) (PVA) (P8136), phosphate buffered saline (PBS) (P4417), dimethyl sulfoxide (DMSO) (472301), dichloromethane (DCM), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-2 H -tetrazolium bromide (MTT) (M2128), coumarin-6 (Cou-6) (442631), folic acid (1039840005) and 1,1-carbonyldiimidazole (115533) were purchased from Sigma-Aldrich.

Techniques: Incubation

Cell viability of HepG2 and MCF-7. Control: cells received no treatment; the chlorambucil-treated cell at CHL concentrations of 6.57 μM, 13.14 μM, and 19.71 μM; F127-folate@PLGA/CHL/IR780; and F127@PLGA/CHL/IR780 at 0.5 mg/mL, 1 mg/mL, and 1.5 mg/mL. Cells were incubated for 48 h (red) and 72 h (blue). Initial cell inoculation: 1000 cells/well; n = 4, * p < 0.05.

Journal: Beilstein Journal of Nanotechnology

Article Title: Therapeutic effect of F127-folate@PLGA/CHL/IR780 nanoparticles on folate receptor-expressing cancer cells

doi: 10.3762/bjnano.15.78

Figure Lengend Snippet: Cell viability of HepG2 and MCF-7. Control: cells received no treatment; the chlorambucil-treated cell at CHL concentrations of 6.57 μM, 13.14 μM, and 19.71 μM; F127-folate@PLGA/CHL/IR780; and F127@PLGA/CHL/IR780 at 0.5 mg/mL, 1 mg/mL, and 1.5 mg/mL. Cells were incubated for 48 h (red) and 72 h (blue). Initial cell inoculation: 1000 cells/well; n = 4, * p < 0.05.

Article Snippet: Chlorambucil (C0253), PLGA 504H (719900), IR780 (425311), Pluronic™ F-127 (P2443), poly(vinyl alcohol) (PVA) (P8136), phosphate buffered saline (PBS) (P4417), dimethyl sulfoxide (DMSO) (472301), dichloromethane (DCM), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-2 H -tetrazolium bromide (MTT) (M2128), coumarin-6 (Cou-6) (442631), folic acid (1039840005) and 1,1-carbonyldiimidazole (115533) were purchased from Sigma-Aldrich.

Techniques: Control, Incubation