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Patterns of 12 unisequences that were identified by <t>microarray</t> and validated with RT-PCR . (G) globular-shape embryo stage; (H) heart-shape embryo stage; (C) Cotyledon stage.
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Patterns of 12 unisequences that were identified by microarray and validated with RT-PCR . (G) globular-shape embryo stage; (H) heart-shape embryo stage; (C) Cotyledon stage.

Journal: BMC Genomics

Article Title: Probing the endosperm gene expression landscape in Brassica napus

doi: 10.1186/1471-2164-10-256

Figure Lengend Snippet: Patterns of 12 unisequences that were identified by microarray and validated with RT-PCR . (G) globular-shape embryo stage; (H) heart-shape embryo stage; (C) Cotyledon stage.

Article Snippet: A set of 10,642 cDNA amplicons was produced and printed in duplicate side-by-side columns on Corning UltraGAPS slides with a Chipwriter Pro microarray printer (Bio-Rad) at the Biotechnology Research Institute (BRI), National Research Council Canada (NRC).

Techniques: Microarray, Reverse Transcription Polymerase Chain Reaction

Correlation of gene expression ratios between cDNA microarray and RT-PCR . The gene expression ratios between tissue-pair comparisons were transformed with log2 ratio.

Journal: BMC Genomics

Article Title: Probing the endosperm gene expression landscape in Brassica napus

doi: 10.1186/1471-2164-10-256

Figure Lengend Snippet: Correlation of gene expression ratios between cDNA microarray and RT-PCR . The gene expression ratios between tissue-pair comparisons were transformed with log2 ratio.

Article Snippet: A set of 10,642 cDNA amplicons was produced and printed in duplicate side-by-side columns on Corning UltraGAPS slides with a Chipwriter Pro microarray printer (Bio-Rad) at the Biotechnology Research Institute (BRI), National Research Council Canada (NRC).

Techniques: Expressing, Microarray, Reverse Transcription Polymerase Chain Reaction, Transformation Assay