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a) A 3-D rendering of the <t>microfluidic</t> chip with a cell-like compartment flanked by two main flow channels to introduce cells, lysis buffer, and a cell-free expression system. b) A schematic of a cell-like compartment. Cells were introduced from the top and retained by a thin capillary (constriction) during centrifugation of the microfluidic chip loaded with a cell suspension in sucrose buffer. The right panel shows a bacterium before and after cell lysis. The cell-free chromosome was labeled with HUα-GFP expressed from plasmids. The fluorescence image after lysis was deconvolved to highlight the blob-like structures. The scale bar applies to both images.
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a) A 3-D rendering of the <t>microfluidic</t> chip with a cell-like compartment flanked by two main flow channels to introduce cells, lysis buffer, and a cell-free expression system. b) A schematic of a cell-like compartment. Cells were introduced from the top and retained by a thin capillary (constriction) during centrifugation of the microfluidic chip loaded with a cell suspension in sucrose buffer. The right panel shows a bacterium before and after cell lysis. The cell-free chromosome was labeled with HUα-GFP expressed from plasmids. The fluorescence image after lysis was deconvolved to highlight the blob-like structures. The scale bar applies to both images.
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a) A 3-D rendering of the <t>microfluidic</t> chip with a cell-like compartment flanked by two main flow channels to introduce cells, lysis buffer, and a cell-free expression system. b) A schematic of a cell-like compartment. Cells were introduced from the top and retained by a thin capillary (constriction) during centrifugation of the microfluidic chip loaded with a cell suspension in sucrose buffer. The right panel shows a bacterium before and after cell lysis. The cell-free chromosome was labeled with HUα-GFP expressed from plasmids. The fluorescence image after lysis was deconvolved to highlight the blob-like structures. The scale bar applies to both images.
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a) A 3-D rendering of the <t>microfluidic</t> chip with a cell-like compartment flanked by two main flow channels to introduce cells, lysis buffer, and a cell-free expression system. b) A schematic of a cell-like compartment. Cells were introduced from the top and retained by a thin capillary (constriction) during centrifugation of the microfluidic chip loaded with a cell suspension in sucrose buffer. The right panel shows a bacterium before and after cell lysis. The cell-free chromosome was labeled with HUα-GFP expressed from plasmids. The fluorescence image after lysis was deconvolved to highlight the blob-like structures. The scale bar applies to both images.
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a) A 3-D rendering of the <t>microfluidic</t> chip with a cell-like compartment flanked by two main flow channels to introduce cells, lysis buffer, and a cell-free expression system. b) A schematic of a cell-like compartment. Cells were introduced from the top and retained by a thin capillary (constriction) during centrifugation of the microfluidic chip loaded with a cell suspension in sucrose buffer. The right panel shows a bacterium before and after cell lysis. The cell-free chromosome was labeled with HUα-GFP expressed from plasmids. The fluorescence image after lysis was deconvolved to highlight the blob-like structures. The scale bar applies to both images.
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a) A 3-D rendering of the <t>microfluidic</t> chip with a cell-like compartment flanked by two main flow channels to introduce cells, lysis buffer, and a cell-free expression system. b) A schematic of a cell-like compartment. Cells were introduced from the top and retained by a thin capillary (constriction) during centrifugation of the microfluidic chip loaded with a cell suspension in sucrose buffer. The right panel shows a bacterium before and after cell lysis. The cell-free chromosome was labeled with HUα-GFP expressed from plasmids. The fluorescence image after lysis was deconvolved to highlight the blob-like structures. The scale bar applies to both images.
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mobile communication chip layout  (Tsinghua Unigroup Ltd)
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a) A 3-D rendering of the <t>microfluidic</t> chip with a cell-like compartment flanked by two main flow channels to introduce cells, lysis buffer, and a cell-free expression system. b) A schematic of a cell-like compartment. Cells were introduced from the top and retained by a thin capillary (constriction) during centrifugation of the microfluidic chip loaded with a cell suspension in sucrose buffer. The right panel shows a bacterium before and after cell lysis. The cell-free chromosome was labeled with HUα-GFP expressed from plasmids. The fluorescence image after lysis was deconvolved to highlight the blob-like structures. The scale bar applies to both images.
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layout chip manufacturing  (Tsinghua Unigroup Ltd)
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a) A 3-D rendering of the <t>microfluidic</t> chip with a cell-like compartment flanked by two main flow channels to introduce cells, lysis buffer, and a cell-free expression system. b) A schematic of a cell-like compartment. Cells were introduced from the top and retained by a thin capillary (constriction) during centrifugation of the microfluidic chip loaded with a cell suspension in sucrose buffer. The right panel shows a bacterium before and after cell lysis. The cell-free chromosome was labeled with HUα-GFP expressed from plasmids. The fluorescence image after lysis was deconvolved to highlight the blob-like structures. The scale bar applies to both images.
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Image Search Results


a) A 3-D rendering of the microfluidic chip with a cell-like compartment flanked by two main flow channels to introduce cells, lysis buffer, and a cell-free expression system. b) A schematic of a cell-like compartment. Cells were introduced from the top and retained by a thin capillary (constriction) during centrifugation of the microfluidic chip loaded with a cell suspension in sucrose buffer. The right panel shows a bacterium before and after cell lysis. The cell-free chromosome was labeled with HUα-GFP expressed from plasmids. The fluorescence image after lysis was deconvolved to highlight the blob-like structures. The scale bar applies to both images.

Journal: bioRxiv

Article Title: Transcription swells chromosomes in vitro

doi: 10.1101/2024.09.25.614905

Figure Lengend Snippet: a) A 3-D rendering of the microfluidic chip with a cell-like compartment flanked by two main flow channels to introduce cells, lysis buffer, and a cell-free expression system. b) A schematic of a cell-like compartment. Cells were introduced from the top and retained by a thin capillary (constriction) during centrifugation of the microfluidic chip loaded with a cell suspension in sucrose buffer. The right panel shows a bacterium before and after cell lysis. The cell-free chromosome was labeled with HUα-GFP expressed from plasmids. The fluorescence image after lysis was deconvolved to highlight the blob-like structures. The scale bar applies to both images.

Article Snippet: We designed the microfluidic chip layout with AutoCAD 2021 (Autodesk) and exposed 5” chrome masks (Nanofilm) with the MicroWriter ML 3 (Durham Magneto Optics Ltd).

Techniques: Introduce, Lysis, Expressing, Centrifugation, Suspension, Labeling, Fluorescence

a) A DNA molecule of the lambda bacteriophage was labeled on specific sites with fluorophores (dC-Cy5, purple star) for imaging and with biotins for surface immobilization in an open microfluidic chip. The surface-immobilized lambda DNA molecule (yellow dashed line) was stretched by flow (yellow solid-line circle). b) A representative fluorescence time-lapse montage shows a DNA molecule before, during, and after a moderate flow pulse. We tracked the position of the fluorescent dye to measure DNA extension, as shown for three different displacement examples. c) The DNA extension by flow was measured after incubating the DNA molecules for 30 minutes in cell-free expression systems with active transcription (Tx), without Tx by adding 500 nM rifampicin, and in phosphate-buffered saline (PBS) solution as a protein-free reference. The data were combined for Tx(ON), Tx(OFF), and buffer from n=3,2,3 independent experiments with mean values as yellow bars and individual molecules as circles.

Journal: bioRxiv

Article Title: Transcription swells chromosomes in vitro

doi: 10.1101/2024.09.25.614905

Figure Lengend Snippet: a) A DNA molecule of the lambda bacteriophage was labeled on specific sites with fluorophores (dC-Cy5, purple star) for imaging and with biotins for surface immobilization in an open microfluidic chip. The surface-immobilized lambda DNA molecule (yellow dashed line) was stretched by flow (yellow solid-line circle). b) A representative fluorescence time-lapse montage shows a DNA molecule before, during, and after a moderate flow pulse. We tracked the position of the fluorescent dye to measure DNA extension, as shown for three different displacement examples. c) The DNA extension by flow was measured after incubating the DNA molecules for 30 minutes in cell-free expression systems with active transcription (Tx), without Tx by adding 500 nM rifampicin, and in phosphate-buffered saline (PBS) solution as a protein-free reference. The data were combined for Tx(ON), Tx(OFF), and buffer from n=3,2,3 independent experiments with mean values as yellow bars and individual molecules as circles.

Article Snippet: We designed the microfluidic chip layout with AutoCAD 2021 (Autodesk) and exposed 5” chrome masks (Nanofilm) with the MicroWriter ML 3 (Durham Magneto Optics Ltd).

Techniques: Labeling, Imaging, Lambda DNA Preparation, Fluorescence, Expressing, Saline