Journal: Cell

Article Title: A spatial code governs olfactory receptor choice and aligns sensory maps in the nose and brain

doi: 10.1016/j.cell.2026.03.051

Figure Lengend Snippet:

Article Snippet: Chromium GEM-X Single Cell 3’ Chip Kit v4 , 10x Genomics , Cat# 1000690.

Techniques: Virus, Olfactory, Recombinant, Single Cell, Gene Expression, Imaging, Staining, Transcriptomics, Library Quantification, Sequencing, Hybridization, Software

Journal: Cell Reports Methods

Article Title: Single chromatin fiber profiling and nucleosome position mapping in the human brain

doi: 10.1016/j.crmeth.2024.100911

Figure Lengend Snippet:

Article Snippet: Chromium GEM-X Single Cell 3' Chip Kit v4 4 chips , 10x Genomics , 1000690.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software, Sequencing

Journal: Cancer Management and Research

Article Title: Long Non-Coding RNA XIST Promotes Wilms Tumor Progression Through the miR-194-5p/YAP Axis

doi: 10.2147/CMAR.S297842

Figure Lengend Snippet: XIST lncRNA, YAP mRNA and protein expression in WT blood and tissue samples. ( A ) ChIP microarray showing differentially expressed lncRNAs in 2 WT and 2 healthy control blood samples. ( B ) RT-qPCR analysis of 49 pairs of matched WT and adjacent non-tumor samples showing the relative expression levels of XIST. ( C ) Kaplan-Meier survival curve of WT patients with differential XIST levels. ( D ) Microarray profiles of differentially expressed mRNA in blood samples of 2 WT patients. ( E and F ) RT-qPCR analysis of the relative expression of YAP mRNA and miR-194-5p in WT tissues and adjacent non-tumor tissues (n = 49 each for tumor vs normal tissue). ( G ) Immunohistochemical (400×magnification) and ( H ) Western blot analyses of YAP protein expression levels in 8 randomly selected pairs of WT tissues and adjacent non-tumor tissues. One-way ANOVA or two-tailed t -test was performed for comparisons between the two groups. ** P < 0.01, *** P < 0.001.

Article Snippet: ChIP was next performed using the human lncRNA array v4 lab-on-a-chip kit (CapitalBio Technology, Beijing, China) according to the manufacturer’s instructions, and the results were analyzed using the GeneSpring software V13.0 (Agilent).

Techniques: Expressing, Microarray, Control, Quantitative RT-PCR, Immunohistochemical staining, Western Blot, Two Tailed Test

Journal: Cancer Management and Research

Article Title: Long Non-Coding RNA XIST Promotes Wilms Tumor Progression Through the miR-194-5p/YAP Axis

doi: 10.2147/CMAR.S297842

Figure Lengend Snippet: Relationship Between XIST Expression and the Clinicopathological Features of WT Patients

Article Snippet: ChIP was next performed using the human lncRNA array v4 lab-on-a-chip kit (CapitalBio Technology, Beijing, China) according to the manufacturer’s instructions, and the results were analyzed using the GeneSpring software V13.0 (Agilent).

Techniques: Expressing

Journal: Cancer Management and Research

Article Title: Long Non-Coding RNA XIST Promotes Wilms Tumor Progression Through the miR-194-5p/YAP Axis

doi: 10.2147/CMAR.S297842

Figure Lengend Snippet: miR-194-5p can bind to both XIST and YAP in WT tissues. XIST lncRNA regulates WT progression through the miR-194-5p/YAP axis. ( A ) XIST 3ʹ-UTR wild-type (XIST-wt) sequence containing the miR-194-5p binding site and sequence of the mutant (XIST-mut) miR-194-5p binding site. ( B and C ) Luciferase reporter gene assay (images and histograms) showed lower luciferase activity for miR-194-5p and XIST-wt than XIST-mut ( P < 0.05). TRAF6 was used as an internal control to verify the integrity of the luciferase gene reporter assay. ( D ) YAP 3ʹ-UTR wild-type (YAP-wt) sequence containing the miR-194-5p binding site and sequence of the mutant (YAP-mut) miR-194-5p binding site. ( E and F ) Luciferase reporter gene assay (images and histograms) showed lower luciferase activity for miR-194-5p and YAP-wt than YAP-mut ( P < 0.05). TRAF6 was used as an internal control to verify the integrity of the luciferase gene reporter assay. ( G ) XIST lncRNA expression and ( H ) miR-194-5p in WT G401 cells and normal renal epithelial HK2 cells. ( I ) RT-qPCR analysis of miR-194-5p after transfection of lentiviral XIST, NC, and sh-XIST in WT G401 cells. ( J ) Western blot analysis showed that YAP protein expression can be regulated by miR-194-5p and XIST. One-way ANOVA or two-tailed t -test was performed for comparisons between the two groups. * P < 0.05, *** P < 0.001.

Article Snippet: ChIP was next performed using the human lncRNA array v4 lab-on-a-chip kit (CapitalBio Technology, Beijing, China) according to the manufacturer’s instructions, and the results were analyzed using the GeneSpring software V13.0 (Agilent).

Techniques: Sequencing, Binding Assay, Mutagenesis, Luciferase, Reporter Gene Assay, Activity Assay, Control, Reporter Assay, Expressing, Quantitative RT-PCR, Transfection, Western Blot, Two Tailed Test