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KEGG and GO analysis of PFKFB4-related genes and the effect of 5MPN on glycolysis in GBM. (A,B) KEGG and GO analysis bubble plots of the top 50 genes most highly correlated with PFKFB4. (C) ATP production in different groups. (D) <t>Oxygen</t> <t>consumption</t> <t>rate</t> in different groups, with the vertical axis showing average fluorescence intensity. (E) ATP production detected by ATP fluorescence probe in different groups. Data in (C,D) are shown as the mean ± SD. n=3. *, P<0.05; ***, P<0.001, Student’s t -test. ATP, adenosine triphosphate; GBM, glioblastoma multiforme; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; NC, negative control; PFKFB4, phosphofructokinase-2/fructose-2,6-bisphosphatase 4; SD, standard deviation.
Character Recognition Ocr Detection Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ABBYY USA Software House Inc optical character recognition editor
KEGG and GO analysis of PFKFB4-related genes and the effect of 5MPN on glycolysis in GBM. (A,B) KEGG and GO analysis bubble plots of the top 50 genes most highly correlated with PFKFB4. (C) ATP production in different groups. (D) <t>Oxygen</t> <t>consumption</t> <t>rate</t> in different groups, with the vertical axis showing average fluorescence intensity. (E) ATP production detected by ATP fluorescence probe in different groups. Data in (C,D) are shown as the mean ± SD. n=3. *, P<0.05; ***, P<0.001, Student’s t -test. ATP, adenosine triphosphate; GBM, glioblastoma multiforme; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; NC, negative control; PFKFB4, phosphofructokinase-2/fructose-2,6-bisphosphatase 4; SD, standard deviation.
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KEGG and GO analysis of PFKFB4-related genes and the effect of 5MPN on glycolysis in GBM. (A,B) KEGG and GO analysis bubble plots of the top 50 genes most highly correlated with PFKFB4. (C) ATP production in different groups. (D) <t>Oxygen</t> <t>consumption</t> <t>rate</t> in different groups, with the vertical axis showing average fluorescence intensity. (E) ATP production detected by ATP fluorescence probe in different groups. Data in (C,D) are shown as the mean ± SD. n=3. *, P<0.05; ***, P<0.001, Student’s t -test. ATP, adenosine triphosphate; GBM, glioblastoma multiforme; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; NC, negative control; PFKFB4, phosphofructokinase-2/fructose-2,6-bisphosphatase 4; SD, standard deviation.
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KEGG and GO analysis of PFKFB4-related genes and the effect of 5MPN on glycolysis in GBM. (A,B) KEGG and GO analysis bubble plots of the top 50 genes most highly correlated with PFKFB4. (C) ATP production in different groups. (D) <t>Oxygen</t> <t>consumption</t> <t>rate</t> in different groups, with the vertical axis showing average fluorescence intensity. (E) ATP production detected by ATP fluorescence probe in different groups. Data in (C,D) are shown as the mean ± SD. n=3. *, P<0.05; ***, P<0.001, Student’s t -test. ATP, adenosine triphosphate; GBM, glioblastoma multiforme; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; NC, negative control; PFKFB4, phosphofructokinase-2/fructose-2,6-bisphosphatase 4; SD, standard deviation.
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KEGG and GO analysis of PFKFB4-related genes and the effect of 5MPN on glycolysis in GBM. (A,B) KEGG and GO analysis bubble plots of the top 50 genes most highly correlated with PFKFB4. (C) ATP production in different groups. (D) <t>Oxygen</t> <t>consumption</t> <t>rate</t> in different groups, with the vertical axis showing average fluorescence intensity. (E) ATP production detected by ATP fluorescence probe in different groups. Data in (C,D) are shown as the mean ± SD. n=3. *, P<0.05; ***, P<0.001, Student’s t -test. ATP, adenosine triphosphate; GBM, glioblastoma multiforme; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; NC, negative control; PFKFB4, phosphofructokinase-2/fructose-2,6-bisphosphatase 4; SD, standard deviation.
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KEGG and GO analysis of PFKFB4-related genes and the effect of 5MPN on glycolysis in GBM. (A,B) KEGG and GO analysis bubble plots of the top 50 genes most highly correlated with PFKFB4. (C) ATP production in different groups. (D) <t>Oxygen</t> <t>consumption</t> <t>rate</t> in different groups, with the vertical axis showing average fluorescence intensity. (E) ATP production detected by ATP fluorescence probe in different groups. Data in (C,D) are shown as the mean ± SD. n=3. *, P<0.05; ***, P<0.001, Student’s t -test. ATP, adenosine triphosphate; GBM, glioblastoma multiforme; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; NC, negative control; PFKFB4, phosphofructokinase-2/fructose-2,6-bisphosphatase 4; SD, standard deviation.
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ABBYY USA Software House Inc a powerful ocr (optical character recognition) software
KEGG and GO analysis of PFKFB4-related genes and the effect of 5MPN on glycolysis in GBM. (A,B) KEGG and GO analysis bubble plots of the top 50 genes most highly correlated with PFKFB4. (C) ATP production in different groups. (D) <t>Oxygen</t> <t>consumption</t> <t>rate</t> in different groups, with the vertical axis showing average fluorescence intensity. (E) ATP production detected by ATP fluorescence probe in different groups. Data in (C,D) are shown as the mean ± SD. n=3. *, P<0.05; ***, P<0.001, Student’s t -test. ATP, adenosine triphosphate; GBM, glioblastoma multiforme; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; NC, negative control; PFKFB4, phosphofructokinase-2/fructose-2,6-bisphosphatase 4; SD, standard deviation.
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Genenet Co Ltd machine learning / optical character recognition (ocr) model
KEGG and GO analysis of PFKFB4-related genes and the effect of 5MPN on glycolysis in GBM. (A,B) KEGG and GO analysis bubble plots of the top 50 genes most highly correlated with PFKFB4. (C) ATP production in different groups. (D) <t>Oxygen</t> <t>consumption</t> <t>rate</t> in different groups, with the vertical axis showing average fluorescence intensity. (E) ATP production detected by ATP fluorescence probe in different groups. Data in (C,D) are shown as the mean ± SD. n=3. *, P<0.05; ***, P<0.001, Student’s t -test. ATP, adenosine triphosphate; GBM, glioblastoma multiforme; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; NC, negative control; PFKFB4, phosphofructokinase-2/fructose-2,6-bisphosphatase 4; SD, standard deviation.
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Hamad Medical Corporation optical character recognition (ocr)
KEGG and GO analysis of PFKFB4-related genes and the effect of 5MPN on glycolysis in GBM. (A,B) KEGG and GO analysis bubble plots of the top 50 genes most highly correlated with PFKFB4. (C) ATP production in different groups. (D) <t>Oxygen</t> <t>consumption</t> <t>rate</t> in different groups, with the vertical axis showing average fluorescence intensity. (E) ATP production detected by ATP fluorescence probe in different groups. Data in (C,D) are shown as the mean ± SD. n=3. *, P<0.05; ***, P<0.001, Student’s t -test. ATP, adenosine triphosphate; GBM, glioblastoma multiforme; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; NC, negative control; PFKFB4, phosphofructokinase-2/fructose-2,6-bisphosphatase 4; SD, standard deviation.
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KEGG and GO analysis of PFKFB4-related genes and the effect of 5MPN on glycolysis in GBM. (A,B) KEGG and GO analysis bubble plots of the top 50 genes most highly correlated with PFKFB4. (C) ATP production in different groups. (D) Oxygen consumption rate in different groups, with the vertical axis showing average fluorescence intensity. (E) ATP production detected by ATP fluorescence probe in different groups. Data in (C,D) are shown as the mean ± SD. n=3. *, P<0.05; ***, P<0.001, Student’s t -test. ATP, adenosine triphosphate; GBM, glioblastoma multiforme; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; NC, negative control; PFKFB4, phosphofructokinase-2/fructose-2,6-bisphosphatase 4; SD, standard deviation.

Journal: Translational Cancer Research

Article Title: 5MPN effectively targets PFKFB4 and inhibits the glucose metabolism process and invasion of glioblastoma

doi: 10.21037/tcr-2025-1740

Figure Lengend Snippet: KEGG and GO analysis of PFKFB4-related genes and the effect of 5MPN on glycolysis in GBM. (A,B) KEGG and GO analysis bubble plots of the top 50 genes most highly correlated with PFKFB4. (C) ATP production in different groups. (D) Oxygen consumption rate in different groups, with the vertical axis showing average fluorescence intensity. (E) ATP production detected by ATP fluorescence probe in different groups. Data in (C,D) are shown as the mean ± SD. n=3. *, P<0.05; ***, P<0.001, Student’s t -test. ATP, adenosine triphosphate; GBM, glioblastoma multiforme; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; NC, negative control; PFKFB4, phosphofructokinase-2/fructose-2,6-bisphosphatase 4; SD, standard deviation.

Article Snippet: ATP detection kit (Beyotime, S0026B) and ATP fluorescence probe (pCMV-Mito-AT1.03, Beyotime, D2606), as well as optical character recognition (OCR) detection kit (Elabscience, Wuhan, China; E-BC-F068), all of which were operated according to the strict instructions provided in the kit manuals.

Techniques: Fluorescence, Negative Control, Standard Deviation