Journal: Science advances

Article Title: Cystic fibrosis alters the structure of the olfactory epithelium and the expression of olfactory receptors affecting odor perception.

doi: 10.1126/sciadv.ads1568

Figure Lengend Snippet: Fig. 2. CFTR is expressed in proliferating human olfactory cells. (A) UMAP projection of the computationally assigned cell cycle scores S (left) and G2M (right). (B) Phase scoring shows an elevated G2M score in the GBC cluster. (C) UMAP projection of the G2M, G1, and S scores (left) and CFTR expression (right) in the GBC cell cluster. (D) CFTR is significantly more expressed in the G1-S phase in the GBC cluster, and G2M score is significantly higher in the CF group; **P < 0.005 and ***P < 0.001 (Mann-Whitney test); n = 41 G1-S and 26 G2M that express CFTR; n = 214 CF and 101 control. (E) Olfactory cell culture after nasal brushing from healthy donors showing CFTR (green) RNA coexpression with DCX, PCNA, and Ki67 antibodies (magenta) visualized by dual in situ hybridization and immunostaining. Scale bars, 20 μM. (F) Effect of the CFTR in- hibitor CFTRinh172 on PCNA and Ki67 immunolabeling showing an increase on the percentage of positive cells. Gaussian probability density functions show a shift toward an increased PCNA and Ki67 fluorescence intensity in CFTRinh172-treated cells. Vertical dashed line indicates the background threshold. n = 1298 (mock), 890 (10 μM), 748 (20 μM), and 885 cells (30 μM); P < 0.001 (Kolmogorov-Smirnov test) in all comparisons. a.u., arbitrary unit; NK, natural killer; DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Cells from two healthy individuals were treated with 0, 10, 20, or 30 μM CFTRinh172 selective CFTR blocker (Tocris) on the culture media and incubated for 24 hours.

Techniques: Expressing, MANN-WHITNEY, Control, Cell Culture, In Situ Hybridization, Immunostaining, Immunolabeling, Fluorescence