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Fluorescence in situ hybridisation (FISH) analyses of selected chromosomes and histological grade in GE1-HCC and GE2-HCC

Journal: BMC Medical Genomics

Article Title: Gene networks and transcriptional regulators associated with liver cancer development and progression

doi: 10.1186/s12920-021-00883-5

Figure Lengend Snippet: Fluorescence in situ hybridisation (FISH) analyses of selected chromosomes and histological grade in GE1-HCC and GE2-HCC

Article Snippet: FISH was performed on 4 μm thick tissue sections using locus-specific probes for centromeres, CEP1, CEP3, CEP7, CEP8, and CEP17 (all supplied by Abbott, Wiesbaden, Germany) according to the supplier’s instructions.

Techniques: Fluorescence, In Situ, Hybridization, Gene Expression

CEP copy number gain detected in fluorescence in situ hybridization. Representative images of CEP1, CEP8, CEP11, and CEP16 copy number gain with an increased number of three or more signals per cell.

Journal: Scientific Reports

Article Title: Centromere 17 copy number gain reflects chromosomal instability in breast cancer

doi: 10.1038/s41598-019-54471-w

Figure Lengend Snippet: CEP copy number gain detected in fluorescence in situ hybridization. Representative images of CEP1, CEP8, CEP11, and CEP16 copy number gain with an increased number of three or more signals per cell.

Article Snippet: FISH using CEP1 [Vysis CEP1 (D1Z5) SpectrumOrange Probe, Abbott Molecular], CEP8 [Vysis CEP8 (D8Z2) SpectrumGreen Probe, Abbott Molecular], CEP11 [Vysis CEP11 (D11Z1) SpectrumGreen Probe, Abbott Molecular], and CEP16 probe [Vysis CEP16 (D16Z3) SpectrumGreen Probe, Abbott Molecular] was performed on TMAs to assess CIN.

Techniques: Fluorescence, In Situ Hybridization