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Transendothelial electrical resistance (TEER) monitored over time after introduction of transwell inserts to the <t>cellZscope</t> the day after seeding of mouse brain microvascular endothelial cells (mBMEC) and four days after astrocyte seeding. Presented are the mean values of n=6 inserts (continuous line) with the standard deviation (SD; dotted lines and filled area) (A) . TEER values obtained at 72h (C) and 120h (D) of incubation in the cellZscope were statistically tested with a repeated measures one-way analysis of variance (ANOVA), followed by Tukey’s test for multiple comparison (C, D) . Barrier permeability assay of inserts after 120h of incubation in the cellZscope . Presented are the values of n=3 inserts with the SD. Horizontal dotted line indicates 1% of FITC-dextran stock solution, whereas horizontal dashed line indicates 0.1% of applied FITC-dextran concentration (B) . FITC-dextran concentrations determined at 240 minutes after apical application were statistically tested with a repeated measures one-way ANOVA, followed by Tukey’s test for multiple comparison (E) . Asterisks indicate statistical significance as follows: p < 0.05*, p < 0.01**, p < 0.0001****.
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Transendothelial electrical resistance (TEER) monitored over time after introduction of transwell inserts to the <t>cellZscope</t> the day after seeding of mouse brain microvascular endothelial cells (mBMEC) and four days after astrocyte seeding. Presented are the mean values of n=6 inserts (continuous line) with the standard deviation (SD; dotted lines and filled area) (A) . TEER values obtained at 72h (C) and 120h (D) of incubation in the cellZscope were statistically tested with a repeated measures one-way analysis of variance (ANOVA), followed by Tukey’s test for multiple comparison (C, D) . Barrier permeability assay of inserts after 120h of incubation in the cellZscope . Presented are the values of n=3 inserts with the SD. Horizontal dotted line indicates 1% of FITC-dextran stock solution, whereas horizontal dashed line indicates 0.1% of applied FITC-dextran concentration (B) . FITC-dextran concentrations determined at 240 minutes after apical application were statistically tested with a repeated measures one-way ANOVA, followed by Tukey’s test for multiple comparison (E) . Asterisks indicate statistical significance as follows: p < 0.05*, p < 0.01**, p < 0.0001****.
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Transendothelial electrical resistance (TEER) monitored over time after introduction of transwell inserts to the cellZscope the day after seeding of mouse brain microvascular endothelial cells (mBMEC) and four days after astrocyte seeding. Presented are the mean values of n=6 inserts (continuous line) with the standard deviation (SD; dotted lines and filled area) (A) . TEER values obtained at 72h (C) and 120h (D) of incubation in the cellZscope were statistically tested with a repeated measures one-way analysis of variance (ANOVA), followed by Tukey’s test for multiple comparison (C, D) . Barrier permeability assay of inserts after 120h of incubation in the cellZscope . Presented are the values of n=3 inserts with the SD. Horizontal dotted line indicates 1% of FITC-dextran stock solution, whereas horizontal dashed line indicates 0.1% of applied FITC-dextran concentration (B) . FITC-dextran concentrations determined at 240 minutes after apical application were statistically tested with a repeated measures one-way ANOVA, followed by Tukey’s test for multiple comparison (E) . Asterisks indicate statistical significance as follows: p < 0.05*, p < 0.01**, p < 0.0001****.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Mosquito- and tick-borne orthoflaviviruses cross an in vitro endothelial-astrocyte barrier

doi: 10.3389/fcimb.2025.1624636

Figure Lengend Snippet: Transendothelial electrical resistance (TEER) monitored over time after introduction of transwell inserts to the cellZscope the day after seeding of mouse brain microvascular endothelial cells (mBMEC) and four days after astrocyte seeding. Presented are the mean values of n=6 inserts (continuous line) with the standard deviation (SD; dotted lines and filled area) (A) . TEER values obtained at 72h (C) and 120h (D) of incubation in the cellZscope were statistically tested with a repeated measures one-way analysis of variance (ANOVA), followed by Tukey’s test for multiple comparison (C, D) . Barrier permeability assay of inserts after 120h of incubation in the cellZscope . Presented are the values of n=3 inserts with the SD. Horizontal dotted line indicates 1% of FITC-dextran stock solution, whereas horizontal dashed line indicates 0.1% of applied FITC-dextran concentration (B) . FITC-dextran concentrations determined at 240 minutes after apical application were statistically tested with a repeated measures one-way ANOVA, followed by Tukey’s test for multiple comparison (E) . Asterisks indicate statistical significance as follows: p < 0.05*, p < 0.01**, p < 0.0001****.

Article Snippet: A day later inserts were transferred into a cellZscope -device ( cellZscope + ® , nanoAnalytics, Münster, Germany) and cultured until they were confluent.

Techniques: Standard Deviation, Incubation, Comparison, Permeability, Concentration Assay