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Journal: Frontiers in Microbiology
Article Title: Antibacterial potential of a new glucosaminidase from Pediococcus acidilactici ITV26 against Gram-negative and Gram-positive pathogens
doi: 10.3389/fmicb.2025.1728482
Figure Lengend Snippet: (a) Evaluation of the antibacterial potential of PGH-enriched extracts obtained from the extracellular (F1), intracellular (F2), and membrane-enriched (F3) fractions against Micrococcus lysodeikticus ATCC 4698. (b) Bacterial growth inhibition halos of the three PGH extracts from Pediococcus acidilactici ITV26 in a Petri dish with LB agar inoculated with M. lysodeikticus ATCC 4698. The membrane-rich F3 fraction is the most active. (c) Native PAGE and zymogram analysis of the F3 PGH extract from P. acidilactici ITV26 using M. lysodeikticus ATCC 4698 cell walls as substrate. 1: Molecular weight marker; 2: electrophoretic profile obtained in membrane-enriched (F3) fraction stained with Coomassie Brilliant Blue R-250; 3: zymography for PGH activity.
Article Snippet: This NAGase activity shows a high
Techniques: Membrane, Inhibition, Clear Native PAGE, Molecular Weight, Marker, Staining, Zymography, Activity Assay