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Invent Biotechnologies minutetm single nucleus isolation kit for tissues/cells
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Invent Biotechnologies minute single nucleus isolation kit
Rpl23a regulates <t>nucleus</t> <t>pulposus</t> cell viability. Cell viability was detected by CCK-8–48 hours after transfection, grouped as Control, shNC, shRpl23a, oeNC, oeRpl23a. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data are expressed as mean ± standard deviation (n=3).
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Invent Biotechnologies buffer b
Rpl23a regulates <t>nucleus</t> <t>pulposus</t> cell viability. Cell viability was detected by CCK-8–48 hours after transfection, grouped as Control, shNC, shRpl23a, oeNC, oeRpl23a. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data are expressed as mean ± standard deviation (n=3).
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Rpl23a regulates nucleus pulposus cell viability. Cell viability was detected by CCK-8–48 hours after transfection, grouped as Control, shNC, shRpl23a, oeNC, oeRpl23a. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data are expressed as mean ± standard deviation (n=3).

Journal: Frontiers in Immunology

Article Title: Swimming ameliorates intervertebral disc degeneration accompanied by Rpl23a downregulation and changes in its immune-inflammatory pathway

doi: 10.3389/fimmu.2026.1819987

Figure Lengend Snippet: Rpl23a regulates nucleus pulposus cell viability. Cell viability was detected by CCK-8–48 hours after transfection, grouped as Control, shNC, shRpl23a, oeNC, oeRpl23a. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data are expressed as mean ± standard deviation (n=3).

Article Snippet: Human nucleus pulposus cells (NPCs, Procell, Cat. No. CP-H097) were routinely cultured in complete medium (Procell, Cat. No. CM-H097) at 37 °C with 5% CO 2 .

Techniques: CCK-8 Assay, Transfection, Control, Standard Deviation

Rpl23a modulates the expression of inflammatory and matrix-related genes in nucleus pulposus cells. RT-qPCR was used to detect the mRNA levels of Rpl23a, TNF-α, IL-1β, IL-6, MMP-13, type II collagen, and aggrecan, with GAPDH as an internal reference. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data are expressed as mean ± standard deviation (n=6), data normalization: normalized to the Control group using the 2^(-ΔΔCt) method with GAPDH as an internal reference.

Journal: Frontiers in Immunology

Article Title: Swimming ameliorates intervertebral disc degeneration accompanied by Rpl23a downregulation and changes in its immune-inflammatory pathway

doi: 10.3389/fimmu.2026.1819987

Figure Lengend Snippet: Rpl23a modulates the expression of inflammatory and matrix-related genes in nucleus pulposus cells. RT-qPCR was used to detect the mRNA levels of Rpl23a, TNF-α, IL-1β, IL-6, MMP-13, type II collagen, and aggrecan, with GAPDH as an internal reference. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data are expressed as mean ± standard deviation (n=6), data normalization: normalized to the Control group using the 2^(-ΔΔCt) method with GAPDH as an internal reference.

Article Snippet: Human nucleus pulposus cells (NPCs, Procell, Cat. No. CP-H097) were routinely cultured in complete medium (Procell, Cat. No. CM-H097) at 37 °C with 5% CO 2 .

Techniques: Expressing, Quantitative RT-PCR, Standard Deviation, Control