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cell fixation protocol (Active Motif)

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Active Motif cell fixation protocol
Cell Fixation Protocol, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+fixation+protocol/cell+fixation+protocol/pm39227386-438-13-20
Average 90 stars, based on 1 article reviews

cell fixation protocol - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Retinoic Acid Is Essential for Th1 Cell Lineage Stability and Prevents Transition to a Th17 Cell Program
Article Snippet: 20-60 million Th1 polarised cells from WT and dnRara mice were fixed, washed and snapfrozen according to the Cell Fixation protocol from Active Motif (http://www.activemotif.com/documents/1848.pdf).

Article Title: PARP-14 Binds Specific DNA Sequences to Promote Th2 Cell Gene Expression
Article Snippet: Cells were then fixed according to the Active Motif (Carlsbad, CA) cell fixation protocol for ChIP assays.

Article Title: TET proteins safeguard bivalent promoters from de novo methylation in human embryonic stem cells
Article Snippet: ~5 × 10 7 cells were fixed, washed and snap-frozen according to the Cell Fixation protocol from Active Motif ( http://www.activemotif.com/documents/1848.pdf ).

Article Title: Specification of type 2 innate lymphocytes by the transcriptional determinant Gfi1.
Article Snippet: Several lines of evidence have documented the existence of a family of innate lymphoid cells (ILCs) that seem to be derived from a common precursor cell dependent on the transcription inhibitor Id2.. ILCs participate in diverse immunological processes, including host defense, the organogenesis of lymphoid tissues, and tissue remodeling and repair1.. Those cells have been proposed to represent the innate counterparts of distinct types of CD4+ helper T lymphocytes on the basis of their production of the specific effector cytokines interferon-γ (IFN-γ), interleukin 5 (IL-5), IL-13, IL-17 or IL-22.

Article Title: Androgens contribute to sex bias of autoimmunity in mice by T cell-intrinsic regulation of Ptpn22 phosphatase expression.
Article Snippet: For each ChIP reaction, approximately 3 × 10^7 cells were fixed, washed, and snap-frozen following the Cell Fixation protocol from Active Motif (http://www.activemotif.com/ documents/1848.pdf).

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Journal: bioRxiv

Article Title: Methanol fixation is the method of choice for droplet-based single-cell transcriptomics of neural cells

doi: 10.1101/2022.08.03.502652

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Article Snippet: Following the methanol fixation protocol for single-cell RNA-seq in 10X Genomics (CG000136), ice-cold DPBS was added to resuspend a 2.5×10 6 cell pellet.

Techniques: Preserving