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Effect of WDR43 knockdown on GC cells. (A) Western blot detection of WDR43 protein expression in GC cells with WDR43 knockdown ( n = 3). (B) Statistical analysis of western blot. (C) CCK-8 detected the proliferation of non-treated GC (CON) and GC cells (siWDR43) after knocking down WDR43 ( n = 3). (D) Invasion assay to detect the invasion ability of cells ( n = 3) (scale bar, 100 μm). (E) Statistical analysis of the results of the invasion experiment ( n = 3). (F) Scratch healing assay to detect cell migration ( n = 3) (scale bar, 500 μm). (G) Statistical analysis of the results of scratch healing experiments ( n = 3). (H) Cell cycle was measured by flow cytometry ( n = 3). (I) Statistical analysis of cell cycle test results ( n = 3). (J) Western blot analysis was performed to detect the levels of CDK2 <t>,</t> <t>cyclinD-1</t> , and DLAT gene expression in GC cells with FDX1 knockout ( n = 3). (K) Statistical analysis of western blot. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, indicating statistically significant differences.
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Effect of WDR43 knockdown on GC cells. (A) Western blot detection of WDR43 protein expression in GC cells with WDR43 knockdown ( n = 3). (B) Statistical analysis of western blot. (C) CCK-8 detected the proliferation of non-treated GC (CON) and GC cells (siWDR43) after knocking down WDR43 ( n = 3). (D) Invasion assay to detect the invasion ability of cells ( n = 3) (scale bar, 100 μm). (E) Statistical analysis of the results of the invasion experiment ( n = 3). (F) Scratch healing assay to detect cell migration ( n = 3) (scale bar, 500 μm). (G) Statistical analysis of the results of scratch healing experiments ( n = 3). (H) Cell cycle was measured by flow cytometry ( n = 3). (I) Statistical analysis of cell cycle test results ( n = 3). (J) Western blot analysis was performed to detect the levels of CDK2 <t>,</t> <t>cyclinD-1</t> , and DLAT gene expression in GC cells with FDX1 knockout ( n = 3). (K) Statistical analysis of western blot. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, indicating statistically significant differences.
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Effect of WDR43 knockdown on GC cells. (A) Western blot detection of WDR43 protein expression in GC cells with WDR43 knockdown ( n = 3). (B) Statistical analysis of western blot. (C) CCK-8 detected the proliferation of non-treated GC (CON) and GC cells (siWDR43) after knocking down WDR43 ( n = 3). (D) Invasion assay to detect the invasion ability of cells ( n = 3) (scale bar, 100 μm). (E) Statistical analysis of the results of the invasion experiment ( n = 3). (F) Scratch healing assay to detect cell migration ( n = 3) (scale bar, 500 μm). (G) Statistical analysis of the results of scratch healing experiments ( n = 3). (H) Cell cycle was measured by flow cytometry ( n = 3). (I) Statistical analysis of cell cycle test results ( n = 3). (J) Western blot analysis was performed to detect the levels of CDK2 <t>,</t> <t>cyclinD-1</t> , and DLAT gene expression in GC cells with FDX1 knockout ( n = 3). (K) Statistical analysis of western blot. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, indicating statistically significant differences.
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Effect of WDR43 knockdown on GC cells. (A) Western blot detection of WDR43 protein expression in GC cells with WDR43 knockdown ( n = 3). (B) Statistical analysis of western blot. (C) CCK-8 detected the proliferation of non-treated GC (CON) and GC cells (siWDR43) after knocking down WDR43 ( n = 3). (D) Invasion assay to detect the invasion ability of cells ( n = 3) (scale bar, 100 μm). (E) Statistical analysis of the results of the invasion experiment ( n = 3). (F) Scratch healing assay to detect cell migration ( n = 3) (scale bar, 500 μm). (G) Statistical analysis of the results of scratch healing experiments ( n = 3). (H) Cell cycle was measured by flow cytometry ( n = 3). (I) Statistical analysis of cell cycle test results ( n = 3). (J) Western blot analysis was performed to detect the levels of CDK2 <t>,</t> <t>cyclinD-1</t> , and DLAT gene expression in GC cells with FDX1 knockout ( n = 3). (K) Statistical analysis of western blot. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, indicating statistically significant differences.
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Effect of WDR43 knockdown on GC cells. (A) Western blot detection of WDR43 protein expression in GC cells with WDR43 knockdown ( n = 3). (B) Statistical analysis of western blot. (C) CCK-8 detected the proliferation of non-treated GC (CON) and GC cells (siWDR43) after knocking down WDR43 ( n = 3). (D) Invasion assay to detect the invasion ability of cells ( n = 3) (scale bar, 100 μm). (E) Statistical analysis of the results of the invasion experiment ( n = 3). (F) Scratch healing assay to detect cell migration ( n = 3) (scale bar, 500 μm). (G) Statistical analysis of the results of scratch healing experiments ( n = 3). (H) Cell cycle was measured by flow cytometry ( n = 3). (I) Statistical analysis of cell cycle test results ( n = 3). (J) Western blot analysis was performed to detect the levels of CDK2 <t>,</t> <t>cyclinD-1</t> , and DLAT gene expression in GC cells with FDX1 knockout ( n = 3). (K) Statistical analysis of western blot. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, indicating statistically significant differences.
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Effect of WDR43 knockdown on GC cells. (A) Western blot detection of WDR43 protein expression in GC cells with WDR43 knockdown ( n = 3). (B) Statistical analysis of western blot. (C) CCK-8 detected the proliferation of non-treated GC (CON) and GC cells (siWDR43) after knocking down WDR43 ( n = 3). (D) Invasion assay to detect the invasion ability of cells ( n = 3) (scale bar, 100 μm). (E) Statistical analysis of the results of the invasion experiment ( n = 3). (F) Scratch healing assay to detect cell migration ( n = 3) (scale bar, 500 μm). (G) Statistical analysis of the results of scratch healing experiments ( n = 3). (H) Cell cycle was measured by flow cytometry ( n = 3). (I) Statistical analysis of cell cycle test results ( n = 3). (J) Western blot analysis was performed to detect the levels of CDK2 , cyclinD-1 , and DLAT gene expression in GC cells with FDX1 knockout ( n = 3). (K) Statistical analysis of western blot. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, indicating statistically significant differences.

Journal: Cancer Biology & Medicine

Article Title: FDX1 expression promotes DSF/Cu-induced cuproptosis in gastric cancer cells

doi: 10.20892/j.issn.2095-3941.2025.0323

Figure Lengend Snippet: Effect of WDR43 knockdown on GC cells. (A) Western blot detection of WDR43 protein expression in GC cells with WDR43 knockdown ( n = 3). (B) Statistical analysis of western blot. (C) CCK-8 detected the proliferation of non-treated GC (CON) and GC cells (siWDR43) after knocking down WDR43 ( n = 3). (D) Invasion assay to detect the invasion ability of cells ( n = 3) (scale bar, 100 μm). (E) Statistical analysis of the results of the invasion experiment ( n = 3). (F) Scratch healing assay to detect cell migration ( n = 3) (scale bar, 500 μm). (G) Statistical analysis of the results of scratch healing experiments ( n = 3). (H) Cell cycle was measured by flow cytometry ( n = 3). (I) Statistical analysis of cell cycle test results ( n = 3). (J) Western blot analysis was performed to detect the levels of CDK2 , cyclinD-1 , and DLAT gene expression in GC cells with FDX1 knockout ( n = 3). (K) Statistical analysis of western blot. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, indicating statistically significant differences.

Article Snippet: The subsequent process included separating the proteins through SDS-PAGE, transferring the proteins onto a PVDF membrane, and combining the membrane with primary antibodies against FDX1, DLAT, WDR43, cyclin dependent-kinase 2 (CDK2), cyclinD-1, and β-actin (Proteintech, Wuhan, China).

Techniques: Knockdown, Western Blot, Expressing, Analysis, CCK-8 Assay, Invasion Assay, Migration, Flow Cytometry, Gene Expression, Knock-Out