cdk2 Search Results


cdk2  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc cdk2
Cell cycle analysis of eupatilin on YD-10B cells. ( A ) Cell cycle distribution was analyzed by flow cytometry after treatment with various concentrations of eupatilin (0–50 μM) for 48 h. Histograms showed the proportion of cells in the G 0 /G 1 , S, and G 2 /M phases. The box in panel A indicates the cell cycle phase distribution (%). ( B ) Quantitative analysis was performed to determine the percentage of cells in each phase of the cell cycle after eupatilin treatment. ( C ) mRNA expression levels of Cyclin D1 and <t>CDK2</t> were determined by RT-qPCR analysis. ( D ) Protein expression levels of Cyclin D1, CDK2, and p21 were examined by Western blot analysis. γ-Tubulin and GAPDH were used as loading controls. Relative band intensities were quantified using ImageJ (version 1.54g) software. Data represent the mean ± SD of three independent experiments (*; p < 0.05, **; p < 0.01, ***; p < 0.001 compared with untreated control).
Cdk2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2561s  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc 2561s
Cell cycle analysis of eupatilin on YD-10B cells. ( A ) Cell cycle distribution was analyzed by flow cytometry after treatment with various concentrations of eupatilin (0–50 μM) for 48 h. Histograms showed the proportion of cells in the G 0 /G 1 , S, and G 2 /M phases. The box in panel A indicates the cell cycle phase distribution (%). ( B ) Quantitative analysis was performed to determine the percentage of cells in each phase of the cell cycle after eupatilin treatment. ( C ) mRNA expression levels of Cyclin D1 and <t>CDK2</t> were determined by RT-qPCR analysis. ( D ) Protein expression levels of Cyclin D1, CDK2, and p21 were examined by Western blot analysis. γ-Tubulin and GAPDH were used as loading controls. Relative band intensities were quantified using ImageJ (version 1.54g) software. Data represent the mean ± SD of three independent experiments (*; p < 0.05, **; p < 0.01, ***; p < 0.001 compared with untreated control).
2561s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
2561s - by Bioz Stars, 2026-04
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rabbit anti cdk2 e8j9t  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit anti cdk2 e8j9t
Cell cycle analysis of eupatilin on YD-10B cells. ( A ) Cell cycle distribution was analyzed by flow cytometry after treatment with various concentrations of eupatilin (0–50 μM) for 48 h. Histograms showed the proportion of cells in the G 0 /G 1 , S, and G 2 /M phases. The box in panel A indicates the cell cycle phase distribution (%). ( B ) Quantitative analysis was performed to determine the percentage of cells in each phase of the cell cycle after eupatilin treatment. ( C ) mRNA expression levels of Cyclin D1 and <t>CDK2</t> were determined by RT-qPCR analysis. ( D ) Protein expression levels of Cyclin D1, CDK2, and p21 were examined by Western blot analysis. γ-Tubulin and GAPDH were used as loading controls. Relative band intensities were quantified using ImageJ (version 1.54g) software. Data represent the mean ± SD of three independent experiments (*; p < 0.05, **; p < 0.01, ***; p < 0.001 compared with untreated control).
Rabbit Anti Cdk2 E8j9t, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dominant negative cdk2  (Addgene inc)
86
Addgene inc dominant negative cdk2
Cell cycle analysis of eupatilin on YD-10B cells. ( A ) Cell cycle distribution was analyzed by flow cytometry after treatment with various concentrations of eupatilin (0–50 μM) for 48 h. Histograms showed the proportion of cells in the G 0 /G 1 , S, and G 2 /M phases. The box in panel A indicates the cell cycle phase distribution (%). ( B ) Quantitative analysis was performed to determine the percentage of cells in each phase of the cell cycle after eupatilin treatment. ( C ) mRNA expression levels of Cyclin D1 and <t>CDK2</t> were determined by RT-qPCR analysis. ( D ) Protein expression levels of Cyclin D1, CDK2, and p21 were examined by Western blot analysis. γ-Tubulin and GAPDH were used as loading controls. Relative band intensities were quantified using ImageJ (version 1.54g) software. Data represent the mean ± SD of three independent experiments (*; p < 0.05, **; p < 0.01, ***; p < 0.001 compared with untreated control).
Dominant Negative Cdk2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cdk2  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti cdk2
Cell cycle analysis of eupatilin on YD-10B cells. ( A ) Cell cycle distribution was analyzed by flow cytometry after treatment with various concentrations of eupatilin (0–50 μM) for 48 h. Histograms showed the proportion of cells in the G 0 /G 1 , S, and G 2 /M phases. The box in panel A indicates the cell cycle phase distribution (%). ( B ) Quantitative analysis was performed to determine the percentage of cells in each phase of the cell cycle after eupatilin treatment. ( C ) mRNA expression levels of Cyclin D1 and <t>CDK2</t> were determined by RT-qPCR analysis. ( D ) Protein expression levels of Cyclin D1, CDK2, and p21 were examined by Western blot analysis. γ-Tubulin and GAPDH were used as loading controls. Relative band intensities were quantified using ImageJ (version 1.54g) software. Data represent the mean ± SD of three independent experiments (*; p < 0.05, **; p < 0.01, ***; p < 0.001 compared with untreated control).
Anti Cdk2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdk1 cdk2  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology cdk1 cdk2
Cell cycle analysis of eupatilin on YD-10B cells. ( A ) Cell cycle distribution was analyzed by flow cytometry after treatment with various concentrations of eupatilin (0–50 μM) for 48 h. Histograms showed the proportion of cells in the G 0 /G 1 , S, and G 2 /M phases. The box in panel A indicates the cell cycle phase distribution (%). ( B ) Quantitative analysis was performed to determine the percentage of cells in each phase of the cell cycle after eupatilin treatment. ( C ) mRNA expression levels of Cyclin D1 and <t>CDK2</t> were determined by RT-qPCR analysis. ( D ) Protein expression levels of Cyclin D1, CDK2, and p21 were examined by Western blot analysis. γ-Tubulin and GAPDH were used as loading controls. Relative band intensities were quantified using ImageJ (version 1.54g) software. Data represent the mean ± SD of three independent experiments (*; p < 0.05, **; p < 0.01, ***; p < 0.001 compared with untreated control).
Cdk1 Cdk2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdk2  (Biosynth Carbosynth)
90
Biosynth Carbosynth cdk2
Figure 4. Structure-‐based sequence alignment of <t>CDK2</t> and CDK7 N-‐terminal
Cdk2, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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14174 1 ap  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc 14174 1 ap
Figure 4. Structure-‐based sequence alignment of <t>CDK2</t> and CDK7 N-‐terminal
14174 1 Ap, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdk2  (Addgene inc)
93
Addgene inc cdk2
Figure 4. Structure-‐based sequence alignment of <t>CDK2</t> and CDK7 N-‐terminal
Cdk2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdk 2  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology cdk 2
Figure 4. Structure-‐based sequence alignment of <t>CDK2</t> and CDK7 N-‐terminal
Cdk 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcmv cdk2 ha dn  (Addgene inc)
93
Addgene inc pcmv cdk2 ha dn
Figure 4. Structure-‐based sequence alignment of <t>CDK2</t> and CDK7 N-‐terminal
Pcmv Cdk2 Ha Dn, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cdk2 mouse monoclonal  (Novus Biologicals)
90
Novus Biologicals anti cdk2 mouse monoclonal
Figure 4. Structure-‐based sequence alignment of <t>CDK2</t> and CDK7 N-‐terminal
Anti Cdk2 Mouse Monoclonal, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Cell cycle analysis of eupatilin on YD-10B cells. ( A ) Cell cycle distribution was analyzed by flow cytometry after treatment with various concentrations of eupatilin (0–50 μM) for 48 h. Histograms showed the proportion of cells in the G 0 /G 1 , S, and G 2 /M phases. The box in panel A indicates the cell cycle phase distribution (%). ( B ) Quantitative analysis was performed to determine the percentage of cells in each phase of the cell cycle after eupatilin treatment. ( C ) mRNA expression levels of Cyclin D1 and CDK2 were determined by RT-qPCR analysis. ( D ) Protein expression levels of Cyclin D1, CDK2, and p21 were examined by Western blot analysis. γ-Tubulin and GAPDH were used as loading controls. Relative band intensities were quantified using ImageJ (version 1.54g) software. Data represent the mean ± SD of three independent experiments (*; p < 0.05, **; p < 0.01, ***; p < 0.001 compared with untreated control).

Journal: Molecules

Article Title: Anti-Invasive and Apoptotic Effect of Eupatilin on YD-10B Human Oral Squamous Carcinoma Cells

doi: 10.3390/molecules30244666

Figure Lengend Snippet: Cell cycle analysis of eupatilin on YD-10B cells. ( A ) Cell cycle distribution was analyzed by flow cytometry after treatment with various concentrations of eupatilin (0–50 μM) for 48 h. Histograms showed the proportion of cells in the G 0 /G 1 , S, and G 2 /M phases. The box in panel A indicates the cell cycle phase distribution (%). ( B ) Quantitative analysis was performed to determine the percentage of cells in each phase of the cell cycle after eupatilin treatment. ( C ) mRNA expression levels of Cyclin D1 and CDK2 were determined by RT-qPCR analysis. ( D ) Protein expression levels of Cyclin D1, CDK2, and p21 were examined by Western blot analysis. γ-Tubulin and GAPDH were used as loading controls. Relative band intensities were quantified using ImageJ (version 1.54g) software. Data represent the mean ± SD of three independent experiments (*; p < 0.05, **; p < 0.01, ***; p < 0.001 compared with untreated control).

Article Snippet: Cyclin D1 (#A19038; Abclonal, Wuhan, China), CDK2 (#2546; Cell Signaling Technology, Danvers, MA, USA), p21 (#2947; Cell Signaling Technology), Bax (#14796, Cell Signaling Technology), Bcl-2 (#15071; Cell Signaling Technology), caspase-9 (#A18676; Abclonal) (#9542; Cell Signaling Technology), caspase-3 (#9662; Cell Signaling Technology), cleaved caspase-3 (#9661; Cell Signaling Technology), PARP (#9542; Cell Signaling Technology), MMP-2 (#A6247; Abclonal), MMP-9 (#A0289, Abclonal), γ-Tubulin (#sc-7396; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and GAPDH (#AC033; Abclonal) were diluted 1:1000 and added and reacted at 4 °C for 12 h. Goat anti-mouse horseradish peroxidase (HRP)-conjugated IgG secondary antibody (#7074P2; Cell Signaling Technology), Horse anti-mouse HRP-conjugated IgG secondary antibody (#7076P2; Cell Signaling Technology), and Donkey anti-goat HRP-conjugated IgG secondary antibody (#sc-2020; Santa Cruz Biotechnology) were diluted 1:5000 and reacted at room temperature for 1 h. After that, the chemiluminescence was measured using an enhanced chemiluminescence (ECL) kit (Amersham pharmacia Biotech Ltd., Amersham, UK).

Techniques: Cell Cycle Assay, Flow Cytometry, Expressing, Quantitative RT-PCR, Western Blot, Software, Control

Figure 4. Structure-‐based sequence alignment of CDK2 and CDK7 N-‐terminal

Journal: ChemMedChem

Article Title: Inhibitor Selectivity for Cyclin-Dependent Kinase 7: A Structural, Thermodynamic, and Modelling Study.

doi: 10.1002/cmdc.201600535

Figure Lengend Snippet: Figure 4. Structure-‐based sequence alignment of CDK2 and CDK7 N-‐terminal

Article Snippet: Briefly, Rb-‐CTF (ProQinase GmbH) and RNA Polymerase II (Cambridge Research Biochemicals) peptide was used for CDK2 and CDK7 kinase assays, respectively.

Techniques: Sequencing