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Primer sequences for qRT-PCR

Journal: Biomolecules and Biomedicine

Article Title: F13B regulates angiogenesis and tumor progression in hepatocellular carcinoma via the HIF-1α/VEGF pathway

doi: 10.17305/bb.2024.10794

Figure Lengend Snippet: Primer sequences for qRT-PCR

Article Snippet: The primary antibodies used were F13B (cat No: 20269-1-AP), CD31 (cat No: 11265-1-AP), p-VEGFR2/VEGFR2 (cat No: 26415-1-AP), MMP2 (cat No: 10373-2-AP), MMP9 (cat No: 10375-2-AP), p-AKT/AKT (cat No: 28731-1-AP / 10176-2-AP), VEGF (cat No: 66828-1-Ig), Cyclin B1 (cat No: 55004-1-AP), CDC2 (cat No: 15646-1-AP), CDC25 (cat No: 55031-1-AP), p21 (cat No: 10355-1-AP) (1:1000), and Cyclin D1 (cat No: 60186-1-Ig), VEGFA (cat No: 19003-1-AP), p-mTOR/mTOR (cat No: 67778-1-Ig / 66888-1-Ig), HIF-1α (cat No: 20960-1-AP) (1:5000) (Wuhan Sanying Biotechnology, Wuhan, China).

Techniques: Sequencing

Effects of knockdown or overexpression of F13B on cyclin expression in HCC. (A–F) After F13B overexpression, changes in cyclin expression (Cyclin B1, CDC2, CDC25, p21, and Cyclin D1) in HCC cells were measured via qRT-PCR and WB; (G–L) After F13B knockdown, changes in cyclin expression in HCC cells were measured via qRT-PCR and WB. * P < 0.05. HCC: Hepatocellular carcinoma; qRT-PCR: Quantitative real-time polymerase chain reaction; WB: Western blotting.

Journal: Biomolecules and Biomedicine

Article Title: F13B regulates angiogenesis and tumor progression in hepatocellular carcinoma via the HIF-1α/VEGF pathway

doi: 10.17305/bb.2024.10794

Figure Lengend Snippet: Effects of knockdown or overexpression of F13B on cyclin expression in HCC. (A–F) After F13B overexpression, changes in cyclin expression (Cyclin B1, CDC2, CDC25, p21, and Cyclin D1) in HCC cells were measured via qRT-PCR and WB; (G–L) After F13B knockdown, changes in cyclin expression in HCC cells were measured via qRT-PCR and WB. * P < 0.05. HCC: Hepatocellular carcinoma; qRT-PCR: Quantitative real-time polymerase chain reaction; WB: Western blotting.

Article Snippet: The primary antibodies used were F13B (cat No: 20269-1-AP), CD31 (cat No: 11265-1-AP), p-VEGFR2/VEGFR2 (cat No: 26415-1-AP), MMP2 (cat No: 10373-2-AP), MMP9 (cat No: 10375-2-AP), p-AKT/AKT (cat No: 28731-1-AP / 10176-2-AP), VEGF (cat No: 66828-1-Ig), Cyclin B1 (cat No: 55004-1-AP), CDC2 (cat No: 15646-1-AP), CDC25 (cat No: 55031-1-AP), p21 (cat No: 10355-1-AP) (1:1000), and Cyclin D1 (cat No: 60186-1-Ig), VEGFA (cat No: 19003-1-AP), p-mTOR/mTOR (cat No: 67778-1-Ig / 66888-1-Ig), HIF-1α (cat No: 20960-1-AP) (1:5000) (Wuhan Sanying Biotechnology, Wuhan, China).

Techniques: Knockdown, Over Expression, Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Western Blot