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Image Search Results
Journal: PLoS ONE
Article Title: Differential Requirement for the CD45 Splicing Regulator hnRNPLL for Accumulation of NKT and Conventional T Cells
doi: 10.1371/journal.pone.0026440
Figure Lengend Snippet: Representative overlay histograms of (A) CD45 and (B) CD45RA, CD45RB and CD45RC expressions on wild type (+/+) (shaded grey) and Hnrpll thu/thu (thu/thu) (black line) CD4 T cells and NKT cells in the thymus (Data are representative of two independent experiments with n = 2–3 mice per group in each).
Article Snippet: Single cell suspensions from thymus, spleen and liver were labelled with mixtures made of the following antibodies: PE labelled CD1d tetramer loaded with α-galactosylceramide (Sapphire Bioscience) was produced in house using a construct provided by M. Kronenberg (La Jolla Institute for Allergy and Immunology, San Diego, CA), FITC labelled anti-TCRβ (clone H57-597) from BD Pharmingen or APC-Cy7 labelled anti-TCRβ (clone H57-597) from Biolegend, APC, FITC or PE-Cy7 labelled anti-NK1.1 (clone PK136) from BD Pharmingen, Alexa Flour 700 labelled anti-CD4 (clone RM4-5) or APC-Cy7 labelled anti-CD4 (clone GK1.5) from BD Pharmingen, PE-Cy7 labelled anti-CD8 (clone 53-6.7) from Biolegend, Pacific Blue labelled anti-CD44 (clone IM7) from Biolegend, Alexa Flour 700 labelled anti-CD45.1 (clone A20) from Biolegend, PerCP-Cy5.5 labelled anti-CD45.2 (clone 104) from BD Pharmingen, FITC labelled anti-CD45 (clone 30-F11) from BD Pharmingen, PerCP-Cy5.5 labelled anti-mouse/human T-Bet (clone eBio4B10) from eBioscience, FITC labelled rat anti-mouse CD122 from BD Pharmingen, PerCP labelled anti-CD45R/B220 (clone RA3-6B2) from BD Pharmingen, Biotin anti-CD45RA (clone 14.8) from BD Pharmingen, FITC labelled anti-CD45RB (clone 16A) from BD Pharmingen,
Techniques: