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(A) Experimental schematic for B-C . (B) Plot shows the percentage of GC B cells of B220+ cells in mice pre-infused with 3BNC117 IgG2a in concert <t>with</t> <t>anti-CD40L</t> (maroon, for B-C ) or isotype control (pink, for B-C ). (C) Plot shows the percentage of PBs in mice pre-infused with 3BNC117 IgG2a in concert with anti-CD40L or isotype control. (D) Experimental schematic for E-J . (E) Plot shows the percentage of GC B cells of B220+ cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a (+Ab, pink, for E-J ) or no antibody (control, -Ab, grey, for E-J ). (F) Plot shows the number of GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (G) Plot shows the percentage of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (H) Plot shows the number of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (I) Plot shows the percentage of TM4-Core+ cells of GC B cells in Cr2 KO mice pre- infused with 3BNC117 IgG2a or control. (J) Plot shows the number of TM4-Core+ GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. Data is displayed as mean ± SD. Each point represents a single mouse from at least two independent experiments. Statistical significance for B-C was determined using two-tailed unpaired t tests with Welch’s correction. Statistical significance for E-J was determined using two-tailed Mann-Whitney U tests. ns denotes non-significant, * denotes p ≤ 0.05, ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.
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(A) Experimental schematic for B-C . (B) Plot shows the percentage of GC B cells of B220+ cells in mice pre-infused with 3BNC117 IgG2a in concert <t>with</t> <t>anti-CD40L</t> (maroon, for B-C ) or isotype control (pink, for B-C ). (C) Plot shows the percentage of PBs in mice pre-infused with 3BNC117 IgG2a in concert with anti-CD40L or isotype control. (D) Experimental schematic for E-J . (E) Plot shows the percentage of GC B cells of B220+ cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a (+Ab, pink, for E-J ) or no antibody (control, -Ab, grey, for E-J ). (F) Plot shows the number of GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (G) Plot shows the percentage of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (H) Plot shows the number of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (I) Plot shows the percentage of TM4-Core+ cells of GC B cells in Cr2 KO mice pre- infused with 3BNC117 IgG2a or control. (J) Plot shows the number of TM4-Core+ GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. Data is displayed as mean ± SD. Each point represents a single mouse from at least two independent experiments. Statistical significance for B-C was determined using two-tailed unpaired t tests with Welch’s correction. Statistical significance for E-J was determined using two-tailed Mann-Whitney U tests. ns denotes non-significant, * denotes p ≤ 0.05, ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.
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(A) Experimental schematic for B-C . (B) Plot shows the percentage of GC B cells of B220+ cells in mice pre-infused with 3BNC117 IgG2a in concert <t>with</t> <t>anti-CD40L</t> (maroon, for B-C ) or isotype control (pink, for B-C ). (C) Plot shows the percentage of PBs in mice pre-infused with 3BNC117 IgG2a in concert with anti-CD40L or isotype control. (D) Experimental schematic for E-J . (E) Plot shows the percentage of GC B cells of B220+ cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a (+Ab, pink, for E-J ) or no antibody (control, -Ab, grey, for E-J ). (F) Plot shows the number of GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (G) Plot shows the percentage of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (H) Plot shows the number of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (I) Plot shows the percentage of TM4-Core+ cells of GC B cells in Cr2 KO mice pre- infused with 3BNC117 IgG2a or control. (J) Plot shows the number of TM4-Core+ GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. Data is displayed as mean ± SD. Each point represents a single mouse from at least two independent experiments. Statistical significance for B-C was determined using two-tailed unpaired t tests with Welch’s correction. Statistical significance for E-J was determined using two-tailed Mann-Whitney U tests. ns denotes non-significant, * denotes p ≤ 0.05, ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.
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(A) Experimental schematic for B-C . (B) Plot shows the percentage of GC B cells of B220+ cells in mice pre-infused with 3BNC117 IgG2a in concert <t>with</t> <t>anti-CD40L</t> (maroon, for B-C ) or isotype control (pink, for B-C ). (C) Plot shows the percentage of PBs in mice pre-infused with 3BNC117 IgG2a in concert with anti-CD40L or isotype control. (D) Experimental schematic for E-J . (E) Plot shows the percentage of GC B cells of B220+ cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a (+Ab, pink, for E-J ) or no antibody (control, -Ab, grey, for E-J ). (F) Plot shows the number of GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (G) Plot shows the percentage of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (H) Plot shows the number of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (I) Plot shows the percentage of TM4-Core+ cells of GC B cells in Cr2 KO mice pre- infused with 3BNC117 IgG2a or control. (J) Plot shows the number of TM4-Core+ GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. Data is displayed as mean ± SD. Each point represents a single mouse from at least two independent experiments. Statistical significance for B-C was determined using two-tailed unpaired t tests with Welch’s correction. Statistical significance for E-J was determined using two-tailed Mann-Whitney U tests. ns denotes non-significant, * denotes p ≤ 0.05, ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.
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Image Search Results


(A) Experimental schematic for B-C . (B) Plot shows the percentage of GC B cells of B220+ cells in mice pre-infused with 3BNC117 IgG2a in concert with anti-CD40L (maroon, for B-C ) or isotype control (pink, for B-C ). (C) Plot shows the percentage of PBs in mice pre-infused with 3BNC117 IgG2a in concert with anti-CD40L or isotype control. (D) Experimental schematic for E-J . (E) Plot shows the percentage of GC B cells of B220+ cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a (+Ab, pink, for E-J ) or no antibody (control, -Ab, grey, for E-J ). (F) Plot shows the number of GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (G) Plot shows the percentage of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (H) Plot shows the number of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (I) Plot shows the percentage of TM4-Core+ cells of GC B cells in Cr2 KO mice pre- infused with 3BNC117 IgG2a or control. (J) Plot shows the number of TM4-Core+ GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. Data is displayed as mean ± SD. Each point represents a single mouse from at least two independent experiments. Statistical significance for B-C was determined using two-tailed unpaired t tests with Welch’s correction. Statistical significance for E-J was determined using two-tailed Mann-Whitney U tests. ns denotes non-significant, * denotes p ≤ 0.05, ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.

Journal: bioRxiv

Article Title: Fc receptor dependent and independent mechanisms of antibody-mediated enhancement of immune responses

doi: 10.64898/2026.03.27.714269

Figure Lengend Snippet: (A) Experimental schematic for B-C . (B) Plot shows the percentage of GC B cells of B220+ cells in mice pre-infused with 3BNC117 IgG2a in concert with anti-CD40L (maroon, for B-C ) or isotype control (pink, for B-C ). (C) Plot shows the percentage of PBs in mice pre-infused with 3BNC117 IgG2a in concert with anti-CD40L or isotype control. (D) Experimental schematic for E-J . (E) Plot shows the percentage of GC B cells of B220+ cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a (+Ab, pink, for E-J ) or no antibody (control, -Ab, grey, for E-J ). (F) Plot shows the number of GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (G) Plot shows the percentage of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (H) Plot shows the number of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (I) Plot shows the percentage of TM4-Core+ cells of GC B cells in Cr2 KO mice pre- infused with 3BNC117 IgG2a or control. (J) Plot shows the number of TM4-Core+ GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. Data is displayed as mean ± SD. Each point represents a single mouse from at least two independent experiments. Statistical significance for B-C was determined using two-tailed unpaired t tests with Welch’s correction. Statistical significance for E-J was determined using two-tailed Mann-Whitney U tests. ns denotes non-significant, * denotes p ≤ 0.05, ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.

Article Snippet: Anti-CD40L (Clone: MR1, BioXCell BE0017-1) and the armenian hamster isotype control (BioXCell BE0091) were purchased from BioXCell.

Techniques: Control, Two Tailed Test, MANN-WHITNEY