Journal: Cell stem cell

Article Title: CD150 high bone marrow Tregs maintain hematopoietic stem cell quiescence and immune privilege via adenosine

doi: 10.1016/j.stem.2018.01.017

Figure Lengend Snippet: A. Allo- or syn-donor chimerism in the peripheral blood of FoxP3cre CXCR4fl/wt or FoxP3cre CXCR4wt/wt mice nine and 24 weeks after transplantation. Recipient mice (B6) received 3-Gy irradiation followed by intravenous injection of MHC-mismatched B10.A BM cells or syngeneic B6 SJL BM cells (2 × 107/each). Anti-CD8 mAb was intraperitoneally injected on day –1 and anti-mouse CD40L mAb on day 0. Experiment included four recipients/group. Data were reproduced by an additional experiment (five mice/group).

Article Snippet: Anti-CD8 mAbs and anti-mouse CD40L mAbs were purchased from BioXcell (West Lebanon, NH, USA).

Techniques: Transplantation Assay, Irradiation, Injection

Journal: Cell stem cell

Article Title: CD150 high bone marrow Tregs maintain hematopoietic stem cell quiescence and immune privilege via adenosine

doi: 10.1016/j.stem.2018.01.017

Figure Lengend Snippet: KEY RESOURCE TABLES

Article Snippet: Anti-CD8 mAbs and anti-mouse CD40L mAbs were purchased from BioXcell (West Lebanon, NH, USA).

Techniques: Recombinant, Reverse Transcription, Gene Expression, Activation Assay, Saline, Red Blood Cell Lysis, Blocking Assay, Software

Journal: Inflammation Research

Article Title: Genetic depletion of the early autophagy protein ATG13 impairs mitochondrial energy metabolism, augments oxidative stress, induces the polarization of macrophages to the M1 inflammatory mode, and compromises myelin integrity in skeletal muscle

doi: 10.1007/s00011-025-02158-6

Figure Lengend Snippet: Polarization of M1Mφ cells in the spleens of Tg+/-ATG13 mice. A Dual IF analysis of CD40 (rabbit anti-CD40; Cat#; ProteinTech; dilution 1:100) and IBA1 (mouse anti-IBA1; Cat#; Invitrogen; dilution 1:100) in 5 μm thick paraffin-embedded spleen sections from 10- to 12-week-old male NTg and Tg+/-ATG13 mice (n=6/group). B Quantification of CD40-ir cells in the 50 μm radius of blood vessels in the red pulp zone was performed. An unpaired t test was performed to verify the significance of the difference in the means between groups, and the results are shown as ****p<0.0005. The results were confirmed after counting 7 independent images per group. C IB followed by D β-actin-normalized densitometric analyses; ***p<0.005 (unpaired t test) between groups. Dual flow cytometry of APC-labeled CD40 and FITC-labeled CD11b in purified Mφs isolated from 10- to 12-week-old E NTg and F Tg+/- ATG13 mice (n=6 male). G The quantification analysis of CD11b+ CD40+ population was performed followed by measuring the significance by unpaired t-test (***p<0.005) . The total number of gated events was 20,000/group. H & I Similarly, dual flow cytometry analysis of CD86 (PE-tagged; dilution 1:100) and CD11b (FITC-tagged; dilution 1:100) followed by J quantification (n=7 analysis/group) was performed (****p<0.0005; unpaired t test). K & L Dual flow cytometry analysis of CD163 (APC-tagged; dilution 1:100) and CD11b (FITC-tagged; dilution 1:100) followed by M quantification (n=7 analysis; ****p<0.0005 by unpaired t test) was performed on purified Mφ cells. The results are presented as the mean ± SD of three experiments

Article Snippet: A Dual IF analysis of CD40 (rabbit anti-CD40; Cat#; ProteinTech; dilution 1:100) and IBA1 (mouse anti-IBA1; Cat#; Invitrogen; dilution 1:100) in 5 μm thick paraffin-embedded spleen sections from 10- to 12-week-old male NTg and Tg+/-ATG13 mice (n=6/group).

Techniques: Flow Cytometry, Labeling, Purification, Isolation

Journal: Inflammation Research

Article Title: Genetic depletion of the early autophagy protein ATG13 impairs mitochondrial energy metabolism, augments oxidative stress, induces the polarization of macrophages to the M1 inflammatory mode, and compromises myelin integrity in skeletal muscle

doi: 10.1007/s00011-025-02158-6

Figure Lengend Snippet: Functional characterization of M1Mφ cells in the spleens of Tg +/−ATG13 mice. Dual IF analysis of IBA1 (mouse anti-IBA1; Cat#; Invitrogen; dilution 1:100) with M1Mφ functional markers, including A iNOS (rabbit anti-CD40; Cat#; ProteinTech; dilution 1:100), B serine 468phospho (S468P) NFκB subunit p65, and C acetylated p65 in paraffin-embedded spleen sections of 10- to 12-week-old NTg and Tg +/−ATG13 mice ( n = 6/group). D Quantification analyses of iNOS (pink bars), S468Pp65 (blue bars), and acetylated p65 (green bars) in n = 6 independent images. Unpaired t tests were used to test the significance of the mean results; *** p < 0.005 and **** p < 0.0005 versus NTg. E 3D surface plots (ImageJ software) were drawn to visualize the fluorescence intensities and the distribution of iNOS, S468Pp65, and acep65-ir cells in the red pulp regions. The red pulp regions were separated from the white pulp regions (arrowhead) by a dotted white line. A distinctively lower distribution of immunoreactive cells was observed in the white pulp regions. The results are presented as the mean ± SD of three experiments

Article Snippet: A Dual IF analysis of CD40 (rabbit anti-CD40; Cat#; ProteinTech; dilution 1:100) and IBA1 (mouse anti-IBA1; Cat#; Invitrogen; dilution 1:100) in 5 μm thick paraffin-embedded spleen sections from 10- to 12-week-old male NTg and Tg+/-ATG13 mice (n=6/group).

Techniques: Functional Assay, Software, Fluorescence

Journal: Inflammation Research

Article Title: Genetic depletion of the early autophagy protein ATG13 impairs mitochondrial energy metabolism, augments oxidative stress, induces the polarization of macrophages to the M1 inflammatory mode, and compromises myelin integrity in skeletal muscle

doi: 10.1007/s00011-025-02158-6

Figure Lengend Snippet: ATG13 ablation mediates perivascular infiltration of M1Mφ cells and may compromise myelin integrity. A Dual IF analysis of the M1Mφ marker CD40 (green; dil 1:100) and the blood vessel endothelium marker laminin alpha-5 (LAMA5; red; 1:100). B Scatter histogram analyses of CD40-ir cells at a 10 μm radius from the outer wall of the blood vessel. The unpaired t test represents the significance of the difference in the mean between two groups, with **** p < 0.0005: n = 13 vessels/group. C Dual IF analysis of the M2Mφ markers CD206 (green) and LAMA5 (red). D Scatter histogram analyses of CD206-ir cells at a 10 μm radius from the outer wall of the blood vessel. NS = not significant according to the count ( n = 13 vessels/group). E LFB staining of myelin (lighter blue) in muscle-serving nerve bundles counterstained with cresyl violet (deep blue) in the biceps femoris muscle of NTg and Tg +/−ATG13 mice ( n = 5/group). F 3D surface plot (ImageJ) showing axonal fibers (cyan circles labeled with red arrowheads) with decreased myelin integrity. The results are presented as the mean ± SEM of three different experiments

Article Snippet: A Dual IF analysis of CD40 (rabbit anti-CD40; Cat#; ProteinTech; dilution 1:100) and IBA1 (mouse anti-IBA1; Cat#; Invitrogen; dilution 1:100) in 5 μm thick paraffin-embedded spleen sections from 10- to 12-week-old male NTg and Tg+/-ATG13 mice (n=6/group).

Techniques: Marker, Staining, Labeling

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Associations Between Mean Platelet Volume and Various Factors in Type 2 Diabetes Patients: A Single-Center Study from Poland

doi: 10.12659/MSM.941109

Figure Lengend Snippet: ( A ) The median TPO concentration in patients with type 2 diabetes did not differ from that of the control group ( P =0.7210). ( B ) The median IL-6 concentration in patients with type 2 diabetes was significantly higher than that of the control group ( P <0.0001). ( C ) The median sP-selectin concentration in patients with type 2 diabetes was significantly higher than that of the control group ( P =0.0008). ( D ) The median sCD40L concentration in patients with type 2 diabetes was significantly higher than that of the control group ( P =0.0010). IL-6 – interleukin 6; sCD40L – soluble form of CD40L; sP-selectin – soluble form of selectin P; TPO – thrombopoietin. Statistical significance: *** P ≤0.001, **** P ≤0.0001. The figure was created with the use of the GraphPad Prism 8.0 software (GraphPad Software, San Diego, CA, USA).

Article Snippet: IL-6 concentration was measured using ELISA Quantikine Human IL-6 Immunoassay kit (Cat#: D6050; R&D Systems Europe Ltd.). sP-selectin concentration was measured using ELISA Quantikine Human P-Selectin Immunoassay kit (Cat#: DPSE00; R&D Systems Europe Ltd.). sCD40L concentration was measured using a human sCD40L ELISA kit (Cat#: 650 120 096; Diaclone SAS, Besançon, France).

Techniques: Concentration Assay, Control, Software

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Associations Between Mean Platelet Volume and Various Factors in Type 2 Diabetes Patients: A Single-Center Study from Poland

doi: 10.12659/MSM.941109

Figure Lengend Snippet: Univariate and multivariate linear regression analysis results for PLT logarithm in patients with type 2 diabetes.

Article Snippet: IL-6 concentration was measured using ELISA Quantikine Human IL-6 Immunoassay kit (Cat#: D6050; R&D Systems Europe Ltd.). sP-selectin concentration was measured using ELISA Quantikine Human P-Selectin Immunoassay kit (Cat#: DPSE00; R&D Systems Europe Ltd.). sCD40L concentration was measured using a human sCD40L ELISA kit (Cat#: 650 120 096; Diaclone SAS, Besançon, France).

Techniques: