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naïve cd4 t cell isolation kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec naïve cd4 t cell isolation kit
    Naïve Cd4 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+kit/Naive+CD4%2B+T+Cell+Isolation+Kit%2C+mouse/pmc13427790-70-17-25
    Average 97 stars, based on 432 article reviews
    naïve cd4 t cell isolation kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: Accurate, sensitive, and efficient chromatin accessibility quantification at target loci using UNIChro-seq.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated with Lymphoprep density gradient medium (STEMCELL, ST07801) and stored at -150°C in CryoStor CS10 (Sigma, C2874-100ML). .. Human primary CD3+ T cells, CD4+ T cells, and B cells were isolated from freshly thawed frozen PBMCs using MACS (Myltenyi, 130-096-535, 130-096-533 and 130-101-638, respectively). .. We sorted PBMCs into eight CD4+ T cell subsets with purity > 95% using a FACS Aria III (BD Biosciences) with the flowcytometry staining panel reported in the ImmuNexUT12 (Supplementary Figure 12 and Supplementary Data 18).

    Article Title: The mechanism of macrophage glycolysis regulating Th17 cell differentiation and promoting the progression of EAN mice.
    Article Snippet: This study investigates how macrophage glycolysis interacts with CD4+ T cells to drive experimental autoimmune neuritis (EAN) progression.. In EAN mice, expression of key glycolytic genes (LDHA, GLUT1, HK2, and PKM2) was gradually upregulated in splenic macrophages and sciatic nerve.. By the disease peak (day 18 p.i.), these genes reaching approximately 4-to-8-fold increase over controls, paralleling a continuous expansion of the splenic M1 macrophage population.

    Article Title: Regulation of Tph cell differentiation via farnesoid X receptor of dendritic cells in inflammatory bowel disease
    Article Snippet: .. After erythrocyte lysis, cell pellets were resuspended and naïve CD4 + T cells were isolated using the Naïve CD4 + T Cell Isolation Kit (130-104-453, Miltenyi, Germany) according to the manufacturer’s instructions. .. For the BMDC–T cell co-culture system, 3 × 10 5 naïve CD4 + T cells from WT or Fxr −/− mice were activated with plate-bound anti-CD3e (16–0031-85, eBioscience, 5ug/ml) for 24 h. They were then co-cultured with 3 × 10 4 BMDCs from WT or Fxr −/− mice with or without IL-12 (10 ng/mL, PeproTech, USA) in 96-well round-bottom plates for 72 h (37°C, 5% CO 2 ).

    Magnetic Cell Separation:

    Article Title: Accurate, sensitive, and efficient chromatin accessibility quantification at target loci using UNIChro-seq.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated with Lymphoprep density gradient medium (STEMCELL, ST07801) and stored at -150°C in CryoStor CS10 (Sigma, C2874-100ML). .. Human primary CD3+ T cells, CD4+ T cells, and B cells were isolated from freshly thawed frozen PBMCs using MACS (Myltenyi, 130-096-535, 130-096-533 and 130-101-638, respectively). .. We sorted PBMCs into eight CD4+ T cell subsets with purity > 95% using a FACS Aria III (BD Biosciences) with the flowcytometry staining panel reported in the ImmuNexUT12 (Supplementary Figure 12 and Supplementary Data 18).

    Cell Isolation:

    Article Title: The mechanism of macrophage glycolysis regulating Th17 cell differentiation and promoting the progression of EAN mice.
    Article Snippet: This study investigates how macrophage glycolysis interacts with CD4+ T cells to drive experimental autoimmune neuritis (EAN) progression.. In EAN mice, expression of key glycolytic genes (LDHA, GLUT1, HK2, and PKM2) was gradually upregulated in splenic macrophages and sciatic nerve.. By the disease peak (day 18 p.i.), these genes reaching approximately 4-to-8-fold increase over controls, paralleling a continuous expansion of the splenic M1 macrophage population.

    Article Title: Prohibitin 2 is a key regulator of T cell proliferation, differentiation, and effector functions in vivo
    Article Snippet: .. CD4 + naïve T cells were purified using the naïve CD4 + T cell Isolation kit for mouse from Miltenyi Biotec (130-104-453). ..

    Article Title: Regulation of Tph cell differentiation via farnesoid X receptor of dendritic cells in inflammatory bowel disease
    Article Snippet: .. After erythrocyte lysis, cell pellets were resuspended and naïve CD4 + T cells were isolated using the Naïve CD4 + T Cell Isolation Kit (130-104-453, Miltenyi, Germany) according to the manufacturer’s instructions. .. For the BMDC–T cell co-culture system, 3 × 10 5 naïve CD4 + T cells from WT or Fxr −/− mice were activated with plate-bound anti-CD3e (16–0031-85, eBioscience, 5ug/ml) for 24 h. They were then co-cultured with 3 × 10 4 BMDCs from WT or Fxr −/− mice with or without IL-12 (10 ng/mL, PeproTech, USA) in 96-well round-bottom plates for 72 h (37°C, 5% CO 2 ).

    Selection:

    Article Title: The mechanism of macrophage glycolysis regulating Th17 cell differentiation and promoting the progression of EAN mice.
    Article Snippet: This study investigates how macrophage glycolysis interacts with CD4+ T cells to drive experimental autoimmune neuritis (EAN) progression.. In EAN mice, expression of key glycolytic genes (LDHA, GLUT1, HK2, and PKM2) was gradually upregulated in splenic macrophages and sciatic nerve.. By the disease peak (day 18 p.i.), these genes reaching approximately 4-to-8-fold increase over controls, paralleling a continuous expansion of the splenic M1 macrophage population.

    Purification:

    Article Title: Prohibitin 2 is a key regulator of T cell proliferation, differentiation, and effector functions in vivo
    Article Snippet: .. CD4 + naïve T cells were purified using the naïve CD4 + T cell Isolation kit for mouse from Miltenyi Biotec (130-104-453). ..

    Lysis:

    Article Title: Regulation of Tph cell differentiation via farnesoid X receptor of dendritic cells in inflammatory bowel disease
    Article Snippet: .. After erythrocyte lysis, cell pellets were resuspended and naïve CD4 + T cells were isolated using the Naïve CD4 + T Cell Isolation Kit (130-104-453, Miltenyi, Germany) according to the manufacturer’s instructions. .. For the BMDC–T cell co-culture system, 3 × 10 5 naïve CD4 + T cells from WT or Fxr −/− mice were activated with plate-bound anti-CD3e (16–0031-85, eBioscience, 5ug/ml) for 24 h. They were then co-cultured with 3 × 10 4 BMDCs from WT or Fxr −/− mice with or without IL-12 (10 ng/mL, PeproTech, USA) in 96-well round-bottom plates for 72 h (37°C, 5% CO 2 ).

    other:

    Article Title: Ligand-Blocking and Agonist Antibodies Targeting TNFR2 Employ Distinct Modes of Action to Induce Antitumor Immunity
    Article Snippet: Mouse CD4+ T cells were isolated from 249 spleens by MACS (CD4+ T cell isola on kit (mouse), Miltenyi Cat# 130-104-454, RRID:AB_3716593).



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    TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic <t>CD4</t> + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.
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    TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic <t>CD4</t> + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.
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    Image Search Results


    TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic CD4 + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.

    Journal: Immunity, Inflammation and Disease

    Article Title: TRPM2 Deficiency Attenuates Allergic Rhinitis‐Like Inflammation With Altered Ca 2+ ‐NFAT Signaling, Treg Responses, and sIgE Production

    doi: 10.1002/iid3.70490

    Figure Lengend Snippet: TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic CD4 + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.

    Article Snippet: Naïve CD4 + T cells were then isolated by negative selection using a commercial mouse CD4 + T Cell Isolation Kit (Cat. No. 130‐104‐454, Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer's instructions.

    Techniques: Activation Assay, Flow Cytometry, Fluorescence, Translocation Assay, Western Blot