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( A-C ) Flow cytometry analysis of <t>CD3</t> + and CD11b + fractions using the indicated antibodies. ( D-F ) Representative images (bottom) show microglia, <t>CD3</t> + <t>T</t> <t>cells,</t> and splenic myeloid cells 7 days after culture following isolation from the brain and spleen (Scale: 20x). Abbreviations: SMCs = spleen myeloid cells; MG = microglia. Please click here to view a larger version of this figure .
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( A-C ) Flow cytometry analysis of <t>CD3</t> + and CD11b + fractions using the indicated antibodies. ( D-F ) Representative images (bottom) show microglia, <t>CD3</t> + <t>T</t> <t>cells,</t> and splenic myeloid cells 7 days after culture following isolation from the brain and spleen (Scale: 20x). Abbreviations: SMCs = spleen myeloid cells; MG = microglia. Please click here to view a larger version of this figure .
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( A-C ) Flow cytometry analysis of <t>CD3</t> + and CD11b + fractions using the indicated antibodies. ( D-F ) Representative images (bottom) show microglia, <t>CD3</t> + <t>T</t> <t>cells,</t> and splenic myeloid cells 7 days after culture following isolation from the brain and spleen (Scale: 20x). Abbreviations: SMCs = spleen myeloid cells; MG = microglia. Please click here to view a larger version of this figure .
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( A-C ) Flow cytometry analysis of <t>CD3</t> + and CD11b + fractions using the indicated antibodies. ( D-F ) Representative images (bottom) show microglia, <t>CD3</t> + <t>T</t> <t>cells,</t> and splenic myeloid cells 7 days after culture following isolation from the brain and spleen (Scale: 20x). Abbreviations: SMCs = spleen myeloid cells; MG = microglia. Please click here to view a larger version of this figure .
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( A-C ) Flow cytometry analysis of <t>CD3</t> + and CD11b + fractions using the indicated antibodies. ( D-F ) Representative images (bottom) show microglia, <t>CD3</t> + <t>T</t> <t>cells,</t> and splenic myeloid cells 7 days after culture following isolation from the brain and spleen (Scale: 20x). Abbreviations: SMCs = spleen myeloid cells; MG = microglia. Please click here to view a larger version of this figure .
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( A-C ) Flow cytometry analysis of <t>CD3</t> + and CD11b + fractions using the indicated antibodies. ( D-F ) Representative images (bottom) show microglia, <t>CD3</t> + <t>T</t> <t>cells,</t> and splenic myeloid cells 7 days after culture following isolation from the brain and spleen (Scale: 20x). Abbreviations: SMCs = spleen myeloid cells; MG = microglia. Please click here to view a larger version of this figure .
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( A-C ) Flow cytometry analysis of <t>CD3</t> + and CD11b + fractions using the indicated antibodies. ( D-F ) Representative images (bottom) show microglia, <t>CD3</t> + <t>T</t> <t>cells,</t> and splenic myeloid cells 7 days after culture following isolation from the brain and spleen (Scale: 20x). Abbreviations: SMCs = spleen myeloid cells; MG = microglia. Please click here to view a larger version of this figure .
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( A-C ) Flow cytometry analysis of <t>CD3</t> + and CD11b + fractions using the indicated antibodies. ( D-F ) Representative images (bottom) show microglia, <t>CD3</t> + <t>T</t> <t>cells,</t> and splenic myeloid cells 7 days after culture following isolation from the brain and spleen (Scale: 20x). Abbreviations: SMCs = spleen myeloid cells; MG = microglia. Please click here to view a larger version of this figure .
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( A-C ) Flow cytometry analysis of <t>CD3</t> + and CD11b + fractions using the indicated antibodies. ( D-F ) Representative images (bottom) show microglia, <t>CD3</t> + <t>T</t> <t>cells,</t> and splenic myeloid cells 7 days after culture following isolation from the brain and spleen (Scale: 20x). Abbreviations: SMCs = spleen myeloid cells; MG = microglia. Please click here to view a larger version of this figure .
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Image Search Results


( A-C ) Flow cytometry analysis of CD3 + and CD11b + fractions using the indicated antibodies. ( D-F ) Representative images (bottom) show microglia, CD3 + T cells, and splenic myeloid cells 7 days after culture following isolation from the brain and spleen (Scale: 20x). Abbreviations: SMCs = spleen myeloid cells; MG = microglia. Please click here to view a larger version of this figure .

Journal: Journal of visualized experiments : JoVE

Article Title: Isolation of Brain Microglia to Study Persistence of Human Immunodeficiency Virus-1

doi: 10.3791/71673

Figure Lengend Snippet: ( A-C ) Flow cytometry analysis of CD3 + and CD11b + fractions using the indicated antibodies. ( D-F ) Representative images (bottom) show microglia, CD3 + T cells, and splenic myeloid cells 7 days after culture following isolation from the brain and spleen (Scale: 20x). Abbreviations: SMCs = spleen myeloid cells; MG = microglia. Please click here to view a larger version of this figure .

Article Snippet: CD3 MicroBead Kit, non-human primate , Miltenyi Biotec , Cat# 130-092-012 , .

Techniques: Isolation, Flow Cytometry, Analysis