Journal: NPJ Regenerative Medicine

Article Title: Adipose-derived dual cell therapy enhances arteriogenesis and limb preservation through vascular integration in critical limb ischemia

doi: 10.1038/s41536-026-00458-x

Figure Lengend Snippet: A , B Western blot analysis for CD141, CD31, VE-cadherin, LYVE1, FGFR2, ETV2, TNAP, αSMA, Transgelin, c-MET, and GAPDH in VMSCs and ADSCs and quantitative densitometry analysis using the NIH ImageJ program. GAPDH was used as a loading control. Values are mean ± SD of 3 independent experiments (* P < 0.05, ** P < 0.01, and *** P < 0.001, n.s.: not significant, Student’s t test). Quantification of HGF ( C ), uPA ( D ), VEGF ( E ), and Angiogenin ( F ) in conditioned medium of VMSCs and ADSCs by ELISA. Values are mean ± SD of 3 independent experiments (* P < 0.05, ** P < 0.01, Student’s t test). G Tube formation assay of VMSC, ADSC, and a combination of VMSC and ADSC in a 2:1 ratio in the presence or absence of 500 pg/ml TNF-α. Scale bar: 500 μm. H Quantitative analysis of tube based on number of meshes, number of master segments, total master segments length, and number of isolated segments using NIH ImageJ angiogenesis analyzer. Values are mean ± SD of 4 independent experiments (*** P < 0.001; V (-TNF-α) VS. V (+TNF-α), $$$ P < 0.001; 2:1 (-TNF-α) VS. 2:1 (+TNF-α), # P < 0.05, ## P < 0.01; n.s.: not significant, One-way ANOVA test followed by Tukey’s multiple comparison test. V: VMSC, A: ADSC.

Article Snippet: The cells were incubated with allophycocyanin (APC)-conjugated antibodies against CD29, CD34, CD44, CD45, CD73, CD90, CD105, and CD141 (Miltenyi Biotec).

Techniques: Western Blot, Control, Enzyme-linked Immunosorbent Assay, Tube Formation Assay, Isolation, Comparison

Journal: NPJ Regenerative Medicine

Article Title: Adipose-derived dual cell therapy enhances arteriogenesis and limb preservation through vascular integration in critical limb ischemia

doi: 10.1038/s41536-026-00458-x

Figure Lengend Snippet: A Western blot of CD141, CD31, αSMA, Transgelin, and GAPDH expression in VMSCs and ADSCs after treatment of 10 ng/ml TNF-α for 24 or 48 h. Quantitative densitometry analysis of CD141 ( B ) CD31 ( C ) αSMA ( D ) and Transgelin ( E ) using NIH ImageJ program. GAPDH was used as a loading control. Each value is expressed as a fold change relative to VMSCs without TNF-α treatment (24 h). Values are mean ± SD of 3 independent experiments. Statistical analyses were conducted exclusively against the respective control groups. (* P < 0.05, *** P < 0.001, Student’s t test). F Gelatin zymography to detect activity of pro-MMP-9, pro-MMP-2, and active-MMP-2 in conditioned medium of VMSCs and ADSCs. Quantitative densitometry analysis of pro-MMP-9 ( G ) pro-MMP-2 ( H ) and active-MMP-2 ( I ). Values are mean ± SD of 3 independent experiments. Total protein lysate (μg) of each group was used as loading control. Statistical analyses were conducted exclusively against the respective control groups. (* P < 0.05, ** P < 0.01, and *** P < 0.001, Student’s t test). Quantification of HGF ( J ) and VEGF ( K ) in conditioned medium in VMSCs and ADSCs by ELISA. Values are mean ± SD of 3 independent experiments. Total protein lysate (μg) of each group was used as loading control. Statistical analyses were conducted exclusively against the respective control groups (** P < 0.01, *** P < 0.001, Student’s t test). L The tube formation assay with VMSC, ADSC and a combination of VMSC (V) and ADSC (A) in a 2:1 ratio was performed after priming of TNF-α for 24 h. Scale bar: 500 μm. M Quantitative analysis of tube architecture was performed based on the number of meshes, the number of master segments, total master segments length, and the number of isolated segments using NIH ImageJ angiogenesis analyzer. Values are mean ± SD of 4 independent experiments (* P < 0.05, ** P < 0.01, *** P < 0.001; VMSC (V) TNF-α (-) VS. V TNF-α (+), $ P < 0.05, $$ P < 0.01; 2:1 TNF-α (-) VS. 2:1 TNF-α (+), # P < 0.05, ## P < 0.01, One-way ANOVA test followed by Tukey’s multiple comparison test). V: VMSC, A: ADSC.

Article Snippet: The cells were incubated with allophycocyanin (APC)-conjugated antibodies against CD29, CD34, CD44, CD45, CD73, CD90, CD105, and CD141 (Miltenyi Biotec).

Techniques: Western Blot, Expressing, Control, Zymography, Activity Assay, Enzyme-linked Immunosorbent Assay, Tube Formation Assay, Isolation, Comparison

Journal: NPJ Regenerative Medicine

Article Title: Adipose-derived dual cell therapy enhances arteriogenesis and limb preservation through vascular integration in critical limb ischemia

doi: 10.1038/s41536-026-00458-x

Figure Lengend Snippet: A Representative fluorescence images of Human/Mouse CD31 + blood vessels at day 28. Scale bar: 100 μm. B , C Quantification of Human/Mouse CD31 + blood vessels analyzed by NIH image J analyzer. Values are mean ± SD of 5 independent experiments (*** P < 0.001, Student’s t test). D Fluorescence images of Human/Mouse Transgelin + blood vessels at day 28. Scale bar: 100 μm. E , F Quantification of Human/Mouse Transgelin + blood vessels analyzed by NIH ImageJ analyzer. Values are mean ± SD of 5 independent experiments (*** P < 0.001, Student’s t test). G Representative fluorescence images of Human CD31 + blood vessels at day 28 post transplantation. Scale bar: 100 μm. Orange scale bar in high mag. image: 50 μm. H Quantification of diameter distribution of human CD31 + blood vessels by NIH ImageJ analyzer. I Double fluorescence staining for Human CD31 and Human/Mouse CD141. White arrow head: colocalization of CD31 and CD141. Scale bar: 50 μm. J The detection of human nuclear antigen (HN) in vessels in dual cell-transplanted muscle. Yellow arrow head indicates human antigen. Scale bar: 20 μm. N = 8/group.

Article Snippet: The cells were incubated with allophycocyanin (APC)-conjugated antibodies against CD29, CD34, CD44, CD45, CD73, CD90, CD105, and CD141 (Miltenyi Biotec).

Techniques: Fluorescence, Transplantation Assay, Staining

Journal: Scientific Reports

Article Title: The immune milieu after local endometrial injury in women with recurrent implantation failure

doi: 10.1038/s41598-025-34198-7

Figure Lengend Snippet: Flow cytometric gating strategy used to identify mononuclear phagocyte and lymphocyte populations. Live cells (DAPI − ) were first selected. Doublets were removed and leukocytes (CD45 + ) were identified ( A ). Mononuclear phagocytes were identified as HLA-DR + and lineage (CD3, CD19, CD20) − . pDC and DC are CD14 − and CD16 − . pDC are CD123 + and CD11c − . CD141 + DC are CD1c − . CD1c + DC are CD141 − (Fig. 2Ai). T cells express CD3. B cells express CD19 and CD20. NK cells express CD56 (Fig. 2Aii). Representative histograms used to further characterise the cellular populations, from n = 1–3 experiments ( B ). CD14 + macrophages also express CD163, CD64, SIRPα/β and CD86(Fig. 2B). Fluorescence minus one (FMO) – negative control.

Article Snippet: I Anti-human CD141 APC , AD5-14H12 , Miltenyi Biotec , 130-113-314.

Techniques: Fluorescence, Negative Control

Journal: Scientific Reports

Article Title: The immune milieu after local endometrial injury in women with recurrent implantation failure

doi: 10.1038/s41598-025-34198-7

Figure Lengend Snippet: Immune cell populations in the endometrial biopsy. Breakdown of the immune cell types in the biopsies ( A ). Comparisons of paired samples for T cells ( B ), NK cells ( C ), CD14 + macrophages ( D ), B cells ( E ), CD141 + DCs ( F ) and CD1c + DCs ( G ).

Article Snippet: I Anti-human CD141 APC , AD5-14H12 , Miltenyi Biotec , 130-113-314.

Techniques:

Journal: Scientific Reports

Article Title: The immune milieu after local endometrial injury in women with recurrent implantation failure

doi: 10.1038/s41598-025-34198-7

Figure Lengend Snippet: Distributions of immune cells. T cells ( A ), NK ( B ), macrophages ( C ), B Cells ( D ), CD141 + DCs ( E ) and CD1c + DCs ( F ) segregated by pregnant and non-pregnant cohorts.

Article Snippet: I Anti-human CD141 APC , AD5-14H12 , Miltenyi Biotec , 130-113-314.

Techniques: