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( A ) Using flow cytometric analysis of fixed whole blood, monocytes were identified as <t>CD14</t> + cells within the CD45 + cell population. CD61 + cells within the CD14 + monocyte population were defined as monocyte-platelet aggregates (MPA). ( B ) shows MPA levels from patients with available MPA levels at both time points (TP). High MPA is defined as ≥ 14.1% and low MPA as ≤ 11.7%.
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( A ) Using flow cytometric analysis of fixed whole blood, monocytes were identified as <t>CD14</t> + cells within the CD45 + cell population. CD61 + cells within the CD14 + monocyte population were defined as monocyte-platelet aggregates (MPA). ( B ) shows MPA levels from patients with available MPA levels at both time points (TP). High MPA is defined as ≥ 14.1% and low MPA as ≤ 11.7%.
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( A ) Using flow cytometric analysis of fixed whole blood, monocytes were identified as <t>CD14</t> + cells within the CD45 + cell population. CD61 + cells within the CD14 + monocyte population were defined as monocyte-platelet aggregates (MPA). ( B ) shows MPA levels from patients with available MPA levels at both time points (TP). High MPA is defined as ≥ 14.1% and low MPA as ≤ 11.7%.
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( A ) Using flow cytometric analysis of fixed whole blood, monocytes were identified as CD14 + cells within the CD45 + cell population. CD61 + cells within the CD14 + monocyte population were defined as monocyte-platelet aggregates (MPA). ( B ) shows MPA levels from patients with available MPA levels at both time points (TP). High MPA is defined as ≥ 14.1% and low MPA as ≤ 11.7%.

Journal: JCI Insight

Article Title: A platelet transcriptomic signature of thromboinflammation predicts cardiovascular risk

doi: 10.1172/jci.insight.195824

Figure Lengend Snippet: ( A ) Using flow cytometric analysis of fixed whole blood, monocytes were identified as CD14 + cells within the CD45 + cell population. CD61 + cells within the CD14 + monocyte population were defined as monocyte-platelet aggregates (MPA). ( B ) shows MPA levels from patients with available MPA levels at both time points (TP). High MPA is defined as ≥ 14.1% and low MPA as ≤ 11.7%.

Article Snippet: Citrate-anticoagulated whole blood was fixed with 1% formalin (Crystalgen catalog CG-190), and stained with CD61 FITC (Agilent DAKO item #F0803), CD86 PE (catalog 555665), CD40 V450 (catalog 561219) (BD), CD14 PE Vio770 (catalog 130-110-521), CD45 VioGreen (catalog 130-110-638), CD16 APC Vio770 (catalog 130-113-390), CD11 APC (catalog 130-110-554), CD162 APC (catalog 130-123-841), and CD142 VioBlue (catalog 130-098-921) (Miltenyi Biotec), followed by lysis of red blood cells.

Techniques: