Journal: iScience

Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s

doi: 10.1016/j.isci.2025.112800

Figure Lengend Snippet: Cleavage under targets and tagmentation sequencing of IRF4 binding in intestinal ILC3 subsets (A) Heatmap showing the genome-wide distribution of IRF4-binding signals at peak centers in ILC3 subsets sorted from Irf4 f/f and Irf4 f/f Rorc cre mice by CUT&Tag. (B) Occupancy of IRF4 at all gene promoter regions (±5 kb of TSS). (C) Donut chart showing the percentages of IRF4 binding at exon regions, intron regions, or intergenic regions. (D) Venn plot displaying the overlap of the IRF4-regulated genes from pairwise comparisons of NKp46 + ILC3s, NKp46 − CCR6 − ILC3s, and CCR6 + ILC3s. (E) Gene set enrichment analysis (GSEA) of NKp46 + ILC3 signature gene sets enriched in shared IRF4-modified genes. (F and G) IGV visualizes the indicated gene locus containing ATAC-seq and IRF4-binding peaks in ILC3 subsets. IRF4 CUT&Tag sequencing data are from two independent replicates. (H–K) Rescue experiments. Retroviruses were generated by transfection of pMX-IRES-GFP plasmids containing the indicated genes into Plat-E cells using PolyJet. CLPs (Lin − CD127 + c-Kit int Sca-1 int Flt3 + ) were sorted from the bone marrow from Irf4 f/f and Irf4f/f Rorc cre mice and transfected with retroviral supernatants. Retrovirus-transfected CLPs were then collected and adoptively transferred into sublethally irradiated CD45.1 + wild-type recipient mice through intravenous tail vein injection. Transduced cells were transferred with CD45.1 + wild-type bone marrow cells to help the engraftment of the CLPs. After 2 weeks, recipient mice were sacrificed, and organs were collected for analysis. (H) Flow cytometry of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (upper). MHC class II expression of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (lower). (I) Cytokine production in siLP CD45.2 + GFP + ILC3s from the indicated recipient mice. (J) The percentages of the indicated subsets were compared. (K) The percentages of MHC class II + ILC3s, IL-17A + ILC3s, and IL-22 + ILC3s were compared. Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments. See also .

Article Snippet: PE-Cyanine5 CD135 (Flt3) Monoclonal Antibody (A2F10) , eBioscience , Cat# 15-1351-82; RRID: AB_494219.

Techniques: Sequencing, Binding Assay, Genome Wide, Modification, Generated, Transfection, Retroviral, Irradiation, Injection, Flow Cytometry, Isolation, Expressing, Two Tailed Test

Journal: Cell reports

Article Title: G2 arrest primes hematopoietic stem cells for megakaryopoiesis

doi: 10.1016/j.celrep.2024.114388

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: PE - CD135 (Flt3) Monoclonal Antibody (A2F10) , Thermo Fisher Scientific , Cat# 12-1351-82; RRID: AB_465859.

Techniques: Recombinant, Adhesive, Purification, Blocking Assay, Amplification, Random Hexamer, Reverse Transcription, SYBR Green Assay, Sterility, Imaging, Software

Journal: Cell Reports Medicine

Article Title: FLT3L-dependent dendritic cells control tumor immunity by modulating Treg and NK cell homeostasis

doi: 10.1016/j.xcrm.2023.101256

Figure Lengend Snippet:

Article Snippet: Anti-CD135 (clone A2F10) , eBioscience , Cat# 13-1351-85; RRID: AB_466600.

Techniques: Control, Recombinant, Staining, Cell Isolation, Software

Journal: Cell Reports Medicine

Article Title: FLT3L-dependent dendritic cells control tumor immunity by modulating Treg and NK cell homeostasis

doi: 10.1016/j.xcrm.2023.101256

Figure Lengend Snippet:

Article Snippet: Anti CD135 (clone A2F10) , eBioscience , Cat# 15-1351-82; RRID: AB_494219.

Techniques: Control, Recombinant, Staining, Cell Isolation, Software

Journal: eLife

Article Title: Regulation of pDC fate determination by histone deacetylase 3

doi: 10.7554/eLife.80477

Figure Lengend Snippet: Reagents.

Article Snippet: FLT3 (CD135) , A2F10 , eBioscience.

Techniques:

Journal: European journal of immunology

Article Title: Guidelines for mouse and human DC generation

doi: 10.1002/eji.202249816

Figure Lengend Snippet: Reagents, antibodies, chemicals, and solutions

Article Snippet: CD135 (Flt3) PerCP-Cy5.5 (A2F10) , eBioscience , 46-1351-82.

Techniques: Recombinant, Saline

Journal: European journal of immunology

Article Title: Guidelines for mouse and human DC generation

doi: 10.1002/eji.202249816

Figure Lengend Snippet: Representative flow cytometry analysis of HoxB8 MPP differentiation into CDP and DC subsets. (A) HoxB8 MPP are cultured in HoxB8 growth medium without E2 for 3 days and analyzed by flow cytometry ( , step 5). MPP: Gr1 − CD117 + CD135 − ; CDP: Gr1 − CD117 int/low CD135 + CD115 + . (B) HoxB8 MPP are cultured in HoxB8 growth medium without E2 for 8 days and analyzed by flow cytometry (see below , step 7). cDC1: Gr1 − CD11c + CD11b low/− XCR1 + ; cDC2: Gr1 − CD11c + CD11b + XCR1 − ; pDC: Gr1 − CD11c + CD11b − B220 + .

Article Snippet: CD135 (Flt3) PerCP-Cy5.5 (A2F10) , eBioscience , 46-1351-82.

Techniques: Flow Cytometry, Cell Culture

Journal: European journal of immunology

Article Title: Guidelines for mouse and human DC generation

doi: 10.1002/eji.202249816

Figure Lengend Snippet: Reagents, enzymes, chemicals, and solutions a)

Article Snippet: CD135 (Flt3) PerCP-Cy5.5 (A2F10) , eBioscience , 46-1351-82.

Techniques: Modification, Saline, Recombinant

Journal: European journal of immunology

Article Title: Guidelines for mouse and human DC generation

doi: 10.1002/eji.202249816

Figure Lengend Snippet: Proposed guidelines to ensure of the identity of the DC types generated in vitro, as compared to cells of the monocyte/macrophage lineages

Article Snippet: CD135 (Flt3) PerCP-Cy5.5 (A2F10) , eBioscience , 46-1351-82.

Techniques: Generated, In Vitro, Functional Assay, Expressing, Immunopeptidomics, Infection, Cell Stimulation