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SL disA increases the antigen-presentation capacity of dendritic cells (A) IFN-β levels in BMDC supernatants from media, SL, SL disA , and SL disA +H151 groups. (B, C) BMDCs double-stained with MHC II and <t>CD11c</t> were analyzed by flow cytometry (B), and CD11c + MHCII + cells are shown in the statistical graph (C). Data were expressed as mean ± SEM, n = 3. ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; one-way ANOVA with Tukey’s multiple comparisons tests.
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SL disA increases the antigen-presentation capacity of dendritic cells (A) IFN-β levels in BMDC supernatants from media, SL, SL disA , and SL disA +H151 groups. (B, C) BMDCs double-stained with MHC II and <t>CD11c</t> were analyzed by flow cytometry (B), and CD11c + MHCII + cells are shown in the statistical graph (C). Data were expressed as mean ± SEM, n = 3. ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; one-way ANOVA with Tukey’s multiple comparisons tests.
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fitc anti mouse cd11c antibody  (Elabscience Biotechnology)
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SL disA increases the antigen-presentation capacity of dendritic cells (A) IFN-β levels in BMDC supernatants from media, SL, SL disA , and SL disA +H151 groups. (B, C) BMDCs double-stained with MHC II and <t>CD11c</t> were analyzed by flow cytometry (B), and CD11c + MHCII + cells are shown in the statistical graph (C). Data were expressed as mean ± SEM, n = 3. ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; one-way ANOVA with Tukey’s multiple comparisons tests.
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SL disA increases the antigen-presentation capacity of dendritic cells (A) IFN-β levels in BMDC supernatants from media, SL, SL disA , and SL disA +H151 groups. (B, C) BMDCs double-stained with MHC II and <t>CD11c</t> were analyzed by flow cytometry (B), and CD11c + MHCII + cells are shown in the statistical graph (C). Data were expressed as mean ± SEM, n = 3. ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; one-way ANOVA with Tukey’s multiple comparisons tests.
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SL disA increases the antigen-presentation capacity of dendritic cells (A) IFN-β levels in BMDC supernatants from media, SL, SL disA , and SL disA +H151 groups. (B, C) BMDCs double-stained with MHC II and <t>CD11c</t> were analyzed by flow cytometry (B), and CD11c + MHCII + cells are shown in the statistical graph (C). Data were expressed as mean ± SEM, n = 3. ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; one-way ANOVA with Tukey’s multiple comparisons tests.
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SL disA increases the antigen-presentation capacity of dendritic cells (A) IFN-β levels in BMDC supernatants from media, SL, SL disA , and SL disA +H151 groups. (B, C) BMDCs double-stained with MHC II and <t>CD11c</t> were analyzed by flow cytometry (B), and CD11c + MHCII + cells are shown in the statistical graph (C). Data were expressed as mean ± SEM, n = 3. ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; one-way ANOVA with Tukey’s multiple comparisons tests.
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SL disA increases the antigen-presentation capacity of dendritic cells (A) IFN-β levels in BMDC supernatants from media, SL, SL disA , and SL disA +H151 groups. (B, C) BMDCs double-stained with MHC II and CD11c were analyzed by flow cytometry (B), and CD11c + MHCII + cells are shown in the statistical graph (C). Data were expressed as mean ± SEM, n = 3. ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; one-way ANOVA with Tukey’s multiple comparisons tests.

Journal: Molecular Therapy Oncology

Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

doi: 10.1016/j.omton.2026.201185

Figure Lengend Snippet: SL disA increases the antigen-presentation capacity of dendritic cells (A) IFN-β levels in BMDC supernatants from media, SL, SL disA , and SL disA +H151 groups. (B, C) BMDCs double-stained with MHC II and CD11c were analyzed by flow cytometry (B), and CD11c + MHCII + cells are shown in the statistical graph (C). Data were expressed as mean ± SEM, n = 3. ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; one-way ANOVA with Tukey’s multiple comparisons tests.

Article Snippet: Cells were subsequently stained with fluorochrome-conjugated antibodies targeting surface markers, including FITC anti-mouse CD11c (clone N418, Elabscience Biotechnology Co., Ltd.) and PE-Cy7 anti-mouse MHC II (clone M5/114, Elabscience Biotechnology Co., Ltd.), and analyzed using a CytoFLEX cytometer (Beckman Coulter, Brea, CA, USA).

Techniques: Immunopeptidomics, Staining, Flow Cytometry