Journal: Frontiers in Cellular Neuroscience

Article Title: Adipose-derived stem cell-conditioned medium mitigates ischemia-induced neuronal injury via the JAK1/STAT3 signaling pathway

doi: 10.3389/fncel.2026.1744887

Figure Lengend Snippet: Characteristics of ADSCs. (A) Passage 3 of cultured ADSCs showed spindle-shaped fibroblast morphology when observed under an inverted microscope. (B) Immunofluorescence staining showed that the ADSCs were positive for the surface antigens CD44 and CD105, while negative for CD34 and CD45. Scale bars: 100 μm (A,B) .

Article Snippet: After washing with PBS, the cells were blocked with 5% normal goat serum (Beyotime, Jiangsu, China) for 30 min, followed by incubation with primary mouse antibodies against CD34 (1:200, sc-65261, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CD44 (1:200, sc-53068, Santa Cruz Biotechnology), CD45 (1:200, sc-1178, Santa Cruz Biotechnology), and CD105 (1:200, sc-20072, Santa Cruz Biotechnology) for 1 h. The cells were then rewashed and incubated with Cy3-conjugated goat anti-mouse IgG (1:400, AS008, ABclonal, Wuhan, China) for 45 min.

Techniques: Cell Culture, Inverted Microscopy, Immunofluorescence, Staining