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Figure 6. The effect of receptor blockers on the effect of 2-ADFP (EC50-EC70) combination with LPI (5 µM) on breast cancer cell line viability. The following substances and concentrations were used: CB1, SR 141716A (100 nM); CB2, SR 144528 (100 nM); GPR55, ML-193 (2 µM); GPR18, PSB CB5 (3 µM); and TRPV1, capsazepine (5 µM). Incubation time 72 h, resazurin test, mean ± standard error (N = 4 experiments). (A) MCF-10A, (B) MCF-7, (C) BT-474, (D) SK-BR-3, (E) BT-20, and (F) MDA-MB- 231. *, a statistically significant difference from 2-ADFP alone, p ≤0.05, ANOVA with Holm–Sidak post-test.

Journal: International journal of molecular sciences

Article Title: Two-Step Cell Death Induction by the New 2-Arachidonoyl Glycerol Analog and Its Modulation by Lysophosphatidylinositol in Human Breast Cancer Cells.

doi: 10.3390/ijms26020820

Figure Lengend Snippet: Figure 6. The effect of receptor blockers on the effect of 2-ADFP (EC50-EC70) combination with LPI (5 µM) on breast cancer cell line viability. The following substances and concentrations were used: CB1, SR 141716A (100 nM); CB2, SR 144528 (100 nM); GPR55, ML-193 (2 µM); GPR18, PSB CB5 (3 µM); and TRPV1, capsazepine (5 µM). Incubation time 72 h, resazurin test, mean ± standard error (N = 4 experiments). (A) MCF-10A, (B) MCF-7, (C) BT-474, (D) SK-BR-3, (E) BT-20, and (F) MDA-MB- 231. *, a statistically significant difference from 2-ADFP alone, p ≤0.05, ANOVA with Holm–Sidak post-test.

Article Snippet: SR 144028, PSB CB5, ML-184, ML-193, capsazepine, and LPI was from Tocris Biosci- ence, Bristol, UK.

Techniques: Incubation

The effect of receptor blockers on the effect of 2-ADFP (EC 50 -EC 70 ) combination with LPI (5 µM) on breast cancer cell line viability. The following substances and concentrations were used: CB1, SR 141716A (100 nM); CB2, SR 144528 (100 nM); GPR55, ML-193 (2 µM); GPR18, PSB CB5 (3 µM); and TRPV1, capsazepine (5 µM). Incubation time 72 h, resazurin test, mean ± standard error ( N = 4 experiments). ( A ) MCF-10A, ( B ) MCF-7, ( C ) BT-474, ( D ) SK-BR-3, ( E ) BT-20, and ( F ) MDA-MB-231. *, a statistically significant difference from 2-ADFP alone, p ≤ 0.05, ANOVA with Holm–Sidak post-test.

Journal: International Journal of Molecular Sciences

Article Title: Two-Step Cell Death Induction by the New 2-Arachidonoyl Glycerol Analog and Its Modulation by Lysophosphatidylinositol in Human Breast Cancer Cells

doi: 10.3390/ijms26020820

Figure Lengend Snippet: The effect of receptor blockers on the effect of 2-ADFP (EC 50 -EC 70 ) combination with LPI (5 µM) on breast cancer cell line viability. The following substances and concentrations were used: CB1, SR 141716A (100 nM); CB2, SR 144528 (100 nM); GPR55, ML-193 (2 µM); GPR18, PSB CB5 (3 µM); and TRPV1, capsazepine (5 µM). Incubation time 72 h, resazurin test, mean ± standard error ( N = 4 experiments). ( A ) MCF-10A, ( B ) MCF-7, ( C ) BT-474, ( D ) SK-BR-3, ( E ) BT-20, and ( F ) MDA-MB-231. *, a statistically significant difference from 2-ADFP alone, p ≤ 0.05, ANOVA with Holm–Sidak post-test.

Article Snippet: SR 144028, PSB CB5, ML-184, ML-193, capsazepine, and LPI was from Tocris Bioscience, Bristol, UK.

Techniques: Incubation