Journal: Mediators of Inflammation

Article Title: Cannabinoid Receptor 2 Protects against Acute Experimental Sepsis in Mice

doi: 10.1155/2013/741303

Figure Lengend Snippet: CB2R protects against LPS-induced shock and proinflammatory cytokines production in mice. (a) CB2R +/+ ( n = 15) and CB2R −/− ( n = 15) mice were challenged with LPS (15 mg/kg, i.p.). Survival was assessed for 24 h. ** P < 0.01. (b)–(d), ELISA detection of serum TNF- α , IL-6 and HMGB1 levels 3 h after LPS (5 mg/kg) challenge in CB2 +/+ ( n = 5) or CB2 −/− ( n = 5) mice. Data are means ± SEM * P < 0.05, and ** P < 0.01. (e) Observation of 72 h survival rate in C57BL/6 mice treated with LPS (30 mg/kg, i.p.) in the absence of presence of indicated doses of GW405833 (i.p.). Mice treated with vehicle as control. n = 15 per group and * P < 0.05 versus vehicle. (f)–(h) ELISA detection of serum TNF- α , IL-6, and HMGB1 levels 6 h after LPS (5 mg/kg, i.p.) challenge in the absence or presence of indicated doses of GW405833 (i.p.). n = 8 in vehicle, n = 5 in 3 mg/kg, n = 10 in 10 mg/kg, data are means ± SEM, and ** P < 0.01 versus vehicle.

Article Snippet: CB2R gene knockout (CB2R −/− ) mice were purchased from Jackson Laboratory (Bar Harbor, Maine, USA) and expanded under specific pathogen-free conditions in Laboratory Animal Centre of Second Military Medical University.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Mediators of Inflammation

Article Title: Cannabinoid Receptor 2 Protects against Acute Experimental Sepsis in Mice

doi: 10.1155/2013/741303

Figure Lengend Snippet: GW405833 inhibits ConA-induced splenocytes proliferation partially via CB2R activation. (a) Splenocytes isolated from CB2R +/+ mice were treated vehicle or ConA (5 μ g/mL) in the absence or presence of GW405833 (1–10 μ M) for 24 h. Splenocytes proliferation was detected by CCK-8. Data are means ± SD ( n = 4) and ** P < 0.01. (b) Splenocytes isolated from CB2R +/+ and CB2R −/− mice were treated with ConA (5 μ g/mL) in the absence or presence of GW405833 (10 μ M) for 24 h. Splenocytes proliferation was detected by CCK-8. Data are means ± SD ( n = 4) and ** P < 0.01.

Article Snippet: CB2R gene knockout (CB2R −/− ) mice were purchased from Jackson Laboratory (Bar Harbor, Maine, USA) and expanded under specific pathogen-free conditions in Laboratory Animal Centre of Second Military Medical University.

Techniques: Activation Assay, Isolation, CCK-8 Assay

Journal: Mediators of Inflammation

Article Title: Cannabinoid Receptor 2 Protects against Acute Experimental Sepsis in Mice

doi: 10.1155/2013/741303

Figure Lengend Snippet: CB2R partially mediates the inhibitory effects of GW405833 on ConA-induced TNF- α and IL-6 production in splenocytes. (a)-(b) Splenocytes isolated from CB2R +/+ mice were treated with vehicle or ConA (5 μ g/mL) in the absence or presence of GW405833 (1–10 μ M) for 24 h. The levels of IL-6 and TNF- α in the supernatants were detected by ELISA. Data are means ± SD ( n = 3), * P < 0.05, and ** P < 0.01 versus ConA. (c)-(d) Splenocytes isolated from CB2R +/+ and CB2R −/− mice were treated with ConA (5 μ g/mL) in the absence or presence of GW405833 (10 μ M) for 24 h. The levels of IL-6 and TNF- α in the supernatants were detected by ELISA. Data are means ± SD ( n = 3), * P < 0.05, and ** P < 0.01.

Article Snippet: CB2R gene knockout (CB2R −/− ) mice were purchased from Jackson Laboratory (Bar Harbor, Maine, USA) and expanded under specific pathogen-free conditions in Laboratory Animal Centre of Second Military Medical University.

Techniques: Isolation, Enzyme-linked Immunosorbent Assay

Journal: Mediators of Inflammation

Article Title: Cannabinoid Receptor 2 Protects against Acute Experimental Sepsis in Mice

doi: 10.1155/2013/741303

Figure Lengend Snippet: CB2R is critical for the inhibitory role of GW405833 on LPS-triggered IL-6, TNF- α , and HMGB1 production in macrophages. (a)–(c) Peritoneal macrophages isolated from CB2R +/+ mice were treated with vehicle or LPS (1 ng/mL) in the absence or presence of GW405833 (1–10 μ M) for 24 h. The levels of IL-6, TNF- α , and HMGB1 in the supernatants were detected by ELISA. (d)–(f) Peritoneal macrophages isolated from CB2R −/− mice were treated as described in (a)–(c). The levels of IL-6, TNF- α , and HMGB1 in the supernatants were detected by ELISA. Data are means ± SD ( n = 3), * P < 0.05, and ** P < 0.01 versus LPS.

Article Snippet: CB2R gene knockout (CB2R −/− ) mice were purchased from Jackson Laboratory (Bar Harbor, Maine, USA) and expanded under specific pathogen-free conditions in Laboratory Animal Centre of Second Military Medical University.

Techniques: Isolation, Enzyme-linked Immunosorbent Assay