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caveolin-1 antibody  (NSJ Bioreagents)
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NSJ Bioreagents caveolin-1 antibody
Caveolin 1 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/caveolin-1/Caveolin-1+Antibody/custom%40rq4919%4042601820
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anti caveolin 1 antibody  (Bioss)
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Bioss anti caveolin 1 antibody
Anti Caveolin 1 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cav1  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc cav1
Cav1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caveolin 1 rabbit pab  (Huabio Inc)
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Huabio Inc caveolin 1 rabbit pab
ATP1A1-Src signaling cascade-induced phosphorylations <t>of</t> <t>Caveolin-1</t> and EGFR are required for PRRSV entry. ( A and B ) Marc-145 cells were treated with 0, 20, 40, 60, 80, and 100 µM PP2 ( A ) and 10, 20, 40, 60, and 80 µM SRCI-1 ( B ) for 48 h. The treated cells were then analyzed using the CCK-8 kit. ( C and D ) The inhibition of SRC-I1 (20 µM) and PP2 (60 µM) on PRRSV infection. Marc-145 cells were pre-treated with two inhibitors for 2 h, followed by PRRSV HuN4-F112 strain (MOI = 1) infection (with compounds present throughout). At 24 hpi, the cell lysates were collected to detect viral RNA abundance via qRT-PCR ( A ), and the supernatants were collected to detect viral titers at 48 hpi ( B ). ( E and F ) The effect of SRC-I1 (10 µM) and PP2 (20 µM) on PRRSV attachment and internalization. Marc-145 cells were pre-treated with two inhibitors for 2 h, then the viral attachment ( E ) and internalization ( F ) assays were conducted in Marc-145 cells. ( G and J ) The activation of the Src-EGFR/caveolin-1 axis upon PRRSV entry. Marc-145 cells were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then, the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( H and K ) The effect of ATP1A1 knockdown on Src-EGFR/caveolin-1 axis activation. Marc-145 cells with or without ATP1A1 knockdown were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( I and L ) The effect of ouabain and PST2238 on Src-EGFR/caveolin-1 axis activation. Marc-145 cells were pretreated with ouabain (25 nM) and PST2238 (25 µM) for 2 h, followed by incubating the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. The cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. Significant differences were indicated as follows: ns ( P > 0.05), * ( P < 0.05), ** ( P < 0.01), *** ( P < 0.001), and **** ( P < 0.0001).
Caveolin 1 Rabbit Pab, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/caveolin-1/anti+atm+mabhuabio+phospho+rabbit+recombinant+s1981/pmc13059770-339-12-40
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caveolin 1 rabbit pab - by Bioz Stars, 2026-09
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primary antibodies targeting caveolin 1  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc primary antibodies targeting caveolin 1
ATP1A1-Src signaling cascade-induced phosphorylations <t>of</t> <t>Caveolin-1</t> and EGFR are required for PRRSV entry. ( A and B ) Marc-145 cells were treated with 0, 20, 40, 60, 80, and 100 µM PP2 ( A ) and 10, 20, 40, 60, and 80 µM SRCI-1 ( B ) for 48 h. The treated cells were then analyzed using the CCK-8 kit. ( C and D ) The inhibition of SRC-I1 (20 µM) and PP2 (60 µM) on PRRSV infection. Marc-145 cells were pre-treated with two inhibitors for 2 h, followed by PRRSV HuN4-F112 strain (MOI = 1) infection (with compounds present throughout). At 24 hpi, the cell lysates were collected to detect viral RNA abundance via qRT-PCR ( A ), and the supernatants were collected to detect viral titers at 48 hpi ( B ). ( E and F ) The effect of SRC-I1 (10 µM) and PP2 (20 µM) on PRRSV attachment and internalization. Marc-145 cells were pre-treated with two inhibitors for 2 h, then the viral attachment ( E ) and internalization ( F ) assays were conducted in Marc-145 cells. ( G and J ) The activation of the Src-EGFR/caveolin-1 axis upon PRRSV entry. Marc-145 cells were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then, the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( H and K ) The effect of ATP1A1 knockdown on Src-EGFR/caveolin-1 axis activation. Marc-145 cells with or without ATP1A1 knockdown were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( I and L ) The effect of ouabain and PST2238 on Src-EGFR/caveolin-1 axis activation. Marc-145 cells were pretreated with ouabain (25 nM) and PST2238 (25 µM) for 2 h, followed by incubating the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. The cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. Significant differences were indicated as follows: ns ( P > 0.05), * ( P < 0.05), ** ( P < 0.01), *** ( P < 0.001), and **** ( P < 0.0001).
Primary Antibodies Targeting Caveolin 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/caveolin-1/Caveolin-1+XP+Rabbit+mAb/pmc12963976-198-11-20
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primary antibodies targeting caveolin 1 - by Bioz Stars, 2026-09
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phospho caveolin 1 y14 rabbit pab  (Huabio Inc)
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Huabio Inc phospho caveolin 1 y14 rabbit pab
ATP1A1-Src signaling cascade-induced phosphorylations <t>of</t> <t>Caveolin-1</t> and EGFR are required for PRRSV entry. ( A and B ) Marc-145 cells were treated with 0, 20, 40, 60, 80, and 100 µM PP2 ( A ) and 10, 20, 40, 60, and 80 µM SRCI-1 ( B ) for 48 h. The treated cells were then analyzed using the CCK-8 kit. ( C and D ) The inhibition of SRC-I1 (20 µM) and PP2 (60 µM) on PRRSV infection. Marc-145 cells were pre-treated with two inhibitors for 2 h, followed by PRRSV HuN4-F112 strain (MOI = 1) infection (with compounds present throughout). At 24 hpi, the cell lysates were collected to detect viral RNA abundance via qRT-PCR ( A ), and the supernatants were collected to detect viral titers at 48 hpi ( B ). ( E and F ) The effect of SRC-I1 (10 µM) and PP2 (20 µM) on PRRSV attachment and internalization. Marc-145 cells were pre-treated with two inhibitors for 2 h, then the viral attachment ( E ) and internalization ( F ) assays were conducted in Marc-145 cells. ( G and J ) The activation of the Src-EGFR/caveolin-1 axis upon PRRSV entry. Marc-145 cells were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then, the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( H and K ) The effect of ATP1A1 knockdown on Src-EGFR/caveolin-1 axis activation. Marc-145 cells with or without ATP1A1 knockdown were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( I and L ) The effect of ouabain and PST2238 on Src-EGFR/caveolin-1 axis activation. Marc-145 cells were pretreated with ouabain (25 nM) and PST2238 (25 µM) for 2 h, followed by incubating the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. The cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. Significant differences were indicated as follows: ns ( P > 0.05), * ( P < 0.05), ** ( P < 0.01), *** ( P < 0.001), and **** ( P < 0.0001).
Phospho Caveolin 1 Y14 Rabbit Pab, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/caveolin-1/anti+atm+mabhuabio+phospho+rabbit+recombinant+s1981/pmc13059770-339-16-40
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santa cruz biotechnology cat  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology santa cruz biotechnology cat
ATP1A1-Src signaling cascade-induced phosphorylations <t>of</t> <t>Caveolin-1</t> and EGFR are required for PRRSV entry. ( A and B ) Marc-145 cells were treated with 0, 20, 40, 60, 80, and 100 µM PP2 ( A ) and 10, 20, 40, 60, and 80 µM SRCI-1 ( B ) for 48 h. The treated cells were then analyzed using the CCK-8 kit. ( C and D ) The inhibition of SRC-I1 (20 µM) and PP2 (60 µM) on PRRSV infection. Marc-145 cells were pre-treated with two inhibitors for 2 h, followed by PRRSV HuN4-F112 strain (MOI = 1) infection (with compounds present throughout). At 24 hpi, the cell lysates were collected to detect viral RNA abundance via qRT-PCR ( A ), and the supernatants were collected to detect viral titers at 48 hpi ( B ). ( E and F ) The effect of SRC-I1 (10 µM) and PP2 (20 µM) on PRRSV attachment and internalization. Marc-145 cells were pre-treated with two inhibitors for 2 h, then the viral attachment ( E ) and internalization ( F ) assays were conducted in Marc-145 cells. ( G and J ) The activation of the Src-EGFR/caveolin-1 axis upon PRRSV entry. Marc-145 cells were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then, the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( H and K ) The effect of ATP1A1 knockdown on Src-EGFR/caveolin-1 axis activation. Marc-145 cells with or without ATP1A1 knockdown were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( I and L ) The effect of ouabain and PST2238 on Src-EGFR/caveolin-1 axis activation. Marc-145 cells were pretreated with ouabain (25 nM) and PST2238 (25 µM) for 2 h, followed by incubating the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. The cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. Significant differences were indicated as follows: ns ( P > 0.05), * ( P < 0.05), ** ( P < 0.01), *** ( P < 0.001), and **** ( P < 0.0001).
Santa Cruz Biotechnology Cat, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/caveolin-1/caveolin-1/pmc12993322-359-42-42
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santa cruz biotechnology cat - by Bioz Stars, 2026-09
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anti caveolin 1 antibodies  (Cytiva Europe)
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Cytiva Europe anti caveolin 1 antibodies
ATP1A1-Src signaling cascade-induced phosphorylations <t>of</t> <t>Caveolin-1</t> and EGFR are required for PRRSV entry. ( A and B ) Marc-145 cells were treated with 0, 20, 40, 60, 80, and 100 µM PP2 ( A ) and 10, 20, 40, 60, and 80 µM SRCI-1 ( B ) for 48 h. The treated cells were then analyzed using the CCK-8 kit. ( C and D ) The inhibition of SRC-I1 (20 µM) and PP2 (60 µM) on PRRSV infection. Marc-145 cells were pre-treated with two inhibitors for 2 h, followed by PRRSV HuN4-F112 strain (MOI = 1) infection (with compounds present throughout). At 24 hpi, the cell lysates were collected to detect viral RNA abundance via qRT-PCR ( A ), and the supernatants were collected to detect viral titers at 48 hpi ( B ). ( E and F ) The effect of SRC-I1 (10 µM) and PP2 (20 µM) on PRRSV attachment and internalization. Marc-145 cells were pre-treated with two inhibitors for 2 h, then the viral attachment ( E ) and internalization ( F ) assays were conducted in Marc-145 cells. ( G and J ) The activation of the Src-EGFR/caveolin-1 axis upon PRRSV entry. Marc-145 cells were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then, the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( H and K ) The effect of ATP1A1 knockdown on Src-EGFR/caveolin-1 axis activation. Marc-145 cells with or without ATP1A1 knockdown were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( I and L ) The effect of ouabain and PST2238 on Src-EGFR/caveolin-1 axis activation. Marc-145 cells were pretreated with ouabain (25 nM) and PST2238 (25 µM) for 2 h, followed by incubating the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. The cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. Significant differences were indicated as follows: ns ( P > 0.05), * ( P < 0.05), ** ( P < 0.01), *** ( P < 0.001), and **** ( P < 0.0001).
Anti Caveolin 1 Antibodies, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/caveolin-1/CNBr-Activated+Sepharose+4B/pm41910268-355-1-11
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anti caveolin 1 antibodies - by Bioz Stars, 2026-09
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cav1  (Proteintech)
95
Proteintech cav1
ATP1A1-Src signaling cascade-induced phosphorylations <t>of</t> <t>Caveolin-1</t> and EGFR are required for PRRSV entry. ( A and B ) Marc-145 cells were treated with 0, 20, 40, 60, 80, and 100 µM PP2 ( A ) and 10, 20, 40, 60, and 80 µM SRCI-1 ( B ) for 48 h. The treated cells were then analyzed using the CCK-8 kit. ( C and D ) The inhibition of SRC-I1 (20 µM) and PP2 (60 µM) on PRRSV infection. Marc-145 cells were pre-treated with two inhibitors for 2 h, followed by PRRSV HuN4-F112 strain (MOI = 1) infection (with compounds present throughout). At 24 hpi, the cell lysates were collected to detect viral RNA abundance via qRT-PCR ( A ), and the supernatants were collected to detect viral titers at 48 hpi ( B ). ( E and F ) The effect of SRC-I1 (10 µM) and PP2 (20 µM) on PRRSV attachment and internalization. Marc-145 cells were pre-treated with two inhibitors for 2 h, then the viral attachment ( E ) and internalization ( F ) assays were conducted in Marc-145 cells. ( G and J ) The activation of the Src-EGFR/caveolin-1 axis upon PRRSV entry. Marc-145 cells were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then, the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( H and K ) The effect of ATP1A1 knockdown on Src-EGFR/caveolin-1 axis activation. Marc-145 cells with or without ATP1A1 knockdown were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( I and L ) The effect of ouabain and PST2238 on Src-EGFR/caveolin-1 axis activation. Marc-145 cells were pretreated with ouabain (25 nM) and PST2238 (25 µM) for 2 h, followed by incubating the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. The cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. Significant differences were indicated as follows: ns ( P > 0.05), * ( P < 0.05), ** ( P < 0.01), *** ( P < 0.001), and **** ( P < 0.0001).
Cav1, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/caveolin-1/Caveolin-1+Antibody/pm41916496-64-13-16
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ATP1A1-Src signaling cascade-induced phosphorylations of Caveolin-1 and EGFR are required for PRRSV entry. ( A and B ) Marc-145 cells were treated with 0, 20, 40, 60, 80, and 100 µM PP2 ( A ) and 10, 20, 40, 60, and 80 µM SRCI-1 ( B ) for 48 h. The treated cells were then analyzed using the CCK-8 kit. ( C and D ) The inhibition of SRC-I1 (20 µM) and PP2 (60 µM) on PRRSV infection. Marc-145 cells were pre-treated with two inhibitors for 2 h, followed by PRRSV HuN4-F112 strain (MOI = 1) infection (with compounds present throughout). At 24 hpi, the cell lysates were collected to detect viral RNA abundance via qRT-PCR ( A ), and the supernatants were collected to detect viral titers at 48 hpi ( B ). ( E and F ) The effect of SRC-I1 (10 µM) and PP2 (20 µM) on PRRSV attachment and internalization. Marc-145 cells were pre-treated with two inhibitors for 2 h, then the viral attachment ( E ) and internalization ( F ) assays were conducted in Marc-145 cells. ( G and J ) The activation of the Src-EGFR/caveolin-1 axis upon PRRSV entry. Marc-145 cells were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then, the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( H and K ) The effect of ATP1A1 knockdown on Src-EGFR/caveolin-1 axis activation. Marc-145 cells with or without ATP1A1 knockdown were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( I and L ) The effect of ouabain and PST2238 on Src-EGFR/caveolin-1 axis activation. Marc-145 cells were pretreated with ouabain (25 nM) and PST2238 (25 µM) for 2 h, followed by incubating the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. The cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. Significant differences were indicated as follows: ns ( P > 0.05), * ( P < 0.05), ** ( P < 0.01), *** ( P < 0.001), and **** ( P < 0.0001).

Journal: mBio

Article Title: ATP1A1 enhances porcine reproductive and respiratory syndrome virus type 2 attachment and internalization

doi: 10.1128/mbio.03896-25

Figure Lengend Snippet: ATP1A1-Src signaling cascade-induced phosphorylations of Caveolin-1 and EGFR are required for PRRSV entry. ( A and B ) Marc-145 cells were treated with 0, 20, 40, 60, 80, and 100 µM PP2 ( A ) and 10, 20, 40, 60, and 80 µM SRCI-1 ( B ) for 48 h. The treated cells were then analyzed using the CCK-8 kit. ( C and D ) The inhibition of SRC-I1 (20 µM) and PP2 (60 µM) on PRRSV infection. Marc-145 cells were pre-treated with two inhibitors for 2 h, followed by PRRSV HuN4-F112 strain (MOI = 1) infection (with compounds present throughout). At 24 hpi, the cell lysates were collected to detect viral RNA abundance via qRT-PCR ( A ), and the supernatants were collected to detect viral titers at 48 hpi ( B ). ( E and F ) The effect of SRC-I1 (10 µM) and PP2 (20 µM) on PRRSV attachment and internalization. Marc-145 cells were pre-treated with two inhibitors for 2 h, then the viral attachment ( E ) and internalization ( F ) assays were conducted in Marc-145 cells. ( G and J ) The activation of the Src-EGFR/caveolin-1 axis upon PRRSV entry. Marc-145 cells were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then, the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( H and K ) The effect of ATP1A1 knockdown on Src-EGFR/caveolin-1 axis activation. Marc-145 cells with or without ATP1A1 knockdown were incubated with the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. Then the cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. ( I and L ) The effect of ouabain and PST2238 on Src-EGFR/caveolin-1 axis activation. Marc-145 cells were pretreated with ouabain (25 nM) and PST2238 (25 µM) for 2 h, followed by incubating the PRRSV HuN4-F112 strain (MOI = 50) at 4°C for 1 h. The cells were washed with cold PBS for six times and transferred to 37°C for the indicated time. Cell lysates were analyzed by Western blot using p-Src, p-EGFR, EGFR, p-caveolin-1, caveolin-1, and β-actin antibodies. Significant differences were indicated as follows: ns ( P > 0.05), * ( P < 0.05), ** ( P < 0.01), *** ( P < 0.001), and **** ( P < 0.0001).

Article Snippet: Phospho-Src-Y419 rabbit mAb (AP1027), EGFR rabbit pAb (A11351), Phospho-EGFR-Y845 rabbit pAb (AP0023), Caveolin-1 rabbit pAb (A1555), Phospho-Caveolin-1-Y14 rabbit pAb (AP0742), rabbit anti-GST-Tag mAb (AE077), β-actin mouse mAb (AC026), and the mCherry rabbit polyclonal antibody ( HA500049 ) was obtained from HUABIO (Hangzhou, China).

Techniques: CCK-8 Assay, Inhibition, Infection, Quantitative RT-PCR, Activation Assay, Incubation, Western Blot, Knockdown

Proposed model of ATP1A1-dependent entry of PRRSV. First, PRRSV specifically recognizes and attaches to the ER4 region of ATP1A1 through GP4. Subsequently, ATP1A1 clusters to recruit more viral particles. At the same time, ATP1A1 activates downstream Src kinase activity, further promoting the phosphorylation of EGFR and Caveolin-1, thereby triggering macropinocytosis and caveolae/raft-mediated endocytosis. CD163 acts as an indispensable co-entry receptor in this process. Finally, ATP1A1, together with viral particles, enters CD163-containing early endosomes, preparing for the subsequent uncoating and genome release.

Journal: mBio

Article Title: ATP1A1 enhances porcine reproductive and respiratory syndrome virus type 2 attachment and internalization

doi: 10.1128/mbio.03896-25

Figure Lengend Snippet: Proposed model of ATP1A1-dependent entry of PRRSV. First, PRRSV specifically recognizes and attaches to the ER4 region of ATP1A1 through GP4. Subsequently, ATP1A1 clusters to recruit more viral particles. At the same time, ATP1A1 activates downstream Src kinase activity, further promoting the phosphorylation of EGFR and Caveolin-1, thereby triggering macropinocytosis and caveolae/raft-mediated endocytosis. CD163 acts as an indispensable co-entry receptor in this process. Finally, ATP1A1, together with viral particles, enters CD163-containing early endosomes, preparing for the subsequent uncoating and genome release.

Article Snippet: Phospho-Src-Y419 rabbit mAb (AP1027), EGFR rabbit pAb (A11351), Phospho-EGFR-Y845 rabbit pAb (AP0023), Caveolin-1 rabbit pAb (A1555), Phospho-Caveolin-1-Y14 rabbit pAb (AP0742), rabbit anti-GST-Tag mAb (AE077), β-actin mouse mAb (AC026), and the mCherry rabbit polyclonal antibody ( HA500049 ) was obtained from HUABIO (Hangzhou, China).

Techniques: Activity Assay, Phospho-proteomics